Madu_00110 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	20,092 / 19,106 / - [in whole cluster] 20,092 / 19,106 / - [in contig]
Location	complement(19106..20092) [in whole cluster] complement(19106..20092) [in contig]
Type	CDS
Length	987 bp (328 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative UDP-glucuronic acid decarboxylase
Product (GenBank)	glucuronic acid decarboxylase
Gene
Gene (GenBank)	mdpA3
EC number
Keyword	madurose
Note
Note (GenBank)
Reference	ACC B0BLM0 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment Maduropeptin生合成遺伝子クラスターの報告 mdpA3: Glucuronic acid decarboxylase mdpA3はD-glucose-1-phosphateから始まる5つの酵素反応で形成されるaminosugarの生合成に関与するとしている。配列から機能推定しているのみ。
Related Reference	ACC Q8KNF5 NITE Calm_00420 PmId [19290519] Characterization of CalS9 in the biosynthesis of UDP-xylose and the production of xylosyl-attached hybrid compound. (Appl Microbiol Biotechnol. , 2009) [26289554] Characterization of Early Enzymes Involved in TDP-Aminodideoxypentose Biosynthesis en Route to Indolocarbazole AT2433. (Chembiochem. , 2015) comment BLAST id61% Micromonospora echinospora_calS9 CalS9 calicheamicin生合成遺伝子 --- [PMID:19290519] CalS9の機能解析論文。 CalS9の特徴付けを3つの方法で調査。・NAD+ cofactor存在下でUDP-GlcAを基質として使用し得られた産物のHPLC解析から、UDP-GlcA → UDP-xyloseの経路を確認。・UDP-Glc, CalS8, NAD+, CalS9を使用し得られた反応産物のHPLC,ESI-MS解析から、UDP-Glc → UDP-GlcA → UDP-xyloseの反応を確認。・thymidyl kinase, acetyl kinase, CalS7, CalS8, CalS9を使用したone-pot assayから得られた産物のHPLC解析から、UMPをスターター基質としてUDP-Glc and UDP-GlcAを経由してUDP-xyloseを形成する経路 (UMP → UDP-Glc, UDP → Glc → UDP-GlcA, UDP-GlcA → UDP-xylose) を確認。いずれのassayもCalS9がUDP-GlcA decarboxylaseであることを証明した。 --- [PMID: 26289554] CalS9は主な産物として、これまで報告されていたUDP-α-D-xyloseよりも、UDP-4-keto-α-D-xyloseを触媒する。
Related Reference	ACC Q0H2W2 PmId [26289554] Characterization of Early Enzymes Involved in TDP-Aminodideoxypentose Biosynthesis en Route to Indolocarbazole AT2433. (Chembiochem. , 2015) comment BLAST id60% Actinomadura melliaura_atS9(atmS9) Putative UDP-glucose 4-epimerase Actinomadura melliauraにおけるAT2433のaminodideoxypentose生合成に関連する・dTDP-α-D-glucose dehydrogenase (AtmS8) ・dTDP-α-D-glucuronic acid decarboxylase (AtmS9) ・dTDP-4-keto-α-D-xylose 2,3-dehydratase (AtmS14) の特徴づけ。 AT2433とcalicheamicinに共通するaminodeoxypentose経路が提唱されている。

Sequence

>putative UDP-glucuronic acid decarboxylase [glucuronic acid decarboxylase]
MERALVTGGSGFIGAHLVDRLVARGDEVTVFDAVPSRDGRGSPREHARHVTGDVRAADAL
AEVIKPGVDVVYHLAAIVGVDRYLTRPLDVIDINVLGTRNVLELAARAGARVLVASTSEV
FGKNPAVPWGEDGDRVLGPTTADRWTYSSSKALAEHMTFAFGRQHGLESRVVRFFNVYGP
RQRPAYIVSRSVHRALNGVPPVVYDRGGQTRCFTYVDDAVEGALLAAGNPAAAGYAFNIG
TTDETTVAEVVALVNKLAGVEATPLQVDTAEKLGPVYEDLPRRVPDTVRAAGLLGWRPGT
GLEDGLTRTIEWARANPWWLSLPDQGAT

>putative UDP-glucuronic acid decarboxylase [glucuronic acid decarboxylase]
ATGGAACGCGCGCTCGTCACGGGCGGCTCGGGGTTCATCGGCGCGCACCTGGTCGACCGG
CTGGTCGCCCGGGGCGACGAGGTGACCGTCTTCGACGCGGTGCCGTCCAGGGACGGCCGC
GGCTCGCCCCGGGAGCACGCCCGCCACGTCACCGGGGACGTCCGCGCCGCCGACGCGCTG
GCCGAGGTGATCAAGCCCGGGGTCGACGTCGTCTACCACCTGGCCGCCATCGTCGGCGTC
GACCGGTACCTCACCCGGCCGCTGGACGTCATCGACATCAACGTGCTCGGCACCCGCAAC
GTGCTGGAGCTGGCGGCCCGCGCGGGCGCCCGGGTGCTGGTCGCCAGCACCAGCGAGGTC
TTCGGCAAGAACCCGGCGGTGCCGTGGGGGGAGGACGGCGACCGGGTGCTCGGCCCGACC
ACGGCCGACCGGTGGACCTACTCGTCCAGCAAGGCGCTCGCCGAGCACATGACGTTCGCG
TTCGGGCGCCAGCACGGGCTGGAGTCCCGGGTGGTGCGCTTCTTCAACGTGTACGGGCCC
CGGCAGCGTCCGGCCTACATCGTCAGCCGCAGCGTGCACCGCGCGCTCAACGGCGTCCCG
CCCGTCGTGTACGACCGGGGCGGGCAGACGCGCTGCTTCACCTACGTGGACGACGCGGTG
GAGGGGGCGCTGCTGGCCGCCGGGAACCCGGCGGCGGCCGGGTACGCGTTCAACATCGGC
ACCACGGACGAGACGACGGTCGCCGAGGTCGTCGCGCTGGTCAACAAGCTCGCCGGGGTC
GAGGCCACGCCCTTGCAGGTGGACACGGCCGAGAAGCTCGGGCCCGTCTACGAGGACCTG
CCGCGCCGGGTGCCCGACACCGTCCGGGCCGCCGGCCTGCTCGGCTGGCGTCCCGGCACC
GGGCTGGAGGACGGCCTGACCAGGACGATCGAGTGGGCGCGGGCGAACCCGTGGTGGCTG
AGCCTGCCGGACCAGGGCGCCACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Madu_00100 Madu_00120