Madu_00120 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	21,438 / 20,095 / - [in whole cluster] 21,438 / 20,095 / - [in contig]
Location	complement(20095..21438) [in whole cluster] complement(20095..21438) [in contig]
Type	CDS
Length	1,344 bp (447 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-alpha-D-glucose dehydrogenase
Product (GenBank)	NDP glucose dehydrogenase
Gene
Gene (GenBank)	mdpA2
EC number	1.1.1.-
Keyword	madurose
Note
Note (GenBank)
Reference	ACC B0BLM1 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment Maduropeptin生合成遺伝子クラスターの報告 mdpA2: NDP-glucose dehydrogenase mdpA2は、D-glucose-1-phosphateから始まり5つの酵素反応で形成されるaminosugarの生合成に関与するとしている。AA配列から機能推定しているのみ。
Related Reference	ACC Q8KNF6 NITE Calm_00410 PmId [12404643] On the origin of deoxypentoses: evidence to support a glucose progenitor in the biosynthesis of calicheamicin. (Chembiochem. , 2002) [26240141] Structural Characterization of CalS8, a TDP-alpha-D-Glucose Dehydrogenase Involved in Calicheamicin Aminodideoxypentose Biosynthesis. (J Biol Chem. , 2015) comment BLAST id52% Micromonospora echinospora_calS8 CalS8 calicheamicin生合成遺伝子 --- [PMID:12404643] CalS8の機能解析報告 calS8を大腸菌にて過剰発現させ、精製し機能解析を行っている。精製酵素のkineticsは他のUDP-alpha-D-glucose dehydrogenase(UDPGlcDH)と一致する一方で、基質としてUDP-alpha-D-glucose and dTDP-alpha-D-glucoseを効率的に利用できることを示した。二次代謝に関与するUDPGlcDHsのなかでは初めての報告。CalS8はNAD+ dependent。 UDPGlcDHではArgが基質特異性を決定する残基であり、GDPManDHはlysineが保存されているのが特徴。CalS8は、Argに対応するアミノ酸残基がLys340であった。しかし、GDPManDH activityは示さなかった。 --- [PMID: 26240141] CalS8の活性測定と構造解明。 CalS8はUDP-GlcよりもdTDP-Glcを15倍以上好む。 aminopentosyltransferase CalG4での報告と併せてcalicheamicin aminopentose経路がdTDP-sugar-dependentな過程であるという支持は高まるが、CalS9(UDP-alpha-D-glucuronic acid decarboxylase)での報告とは対比する。
Related Reference	ACC Q0H2W3 PmId [26289554] Characterization of Early Enzymes Involved in TDP-Aminodideoxypentose Biosynthesis en Route to Indolocarbazole AT2433. (Chembiochem. , 2015) comment BLAST id58% Actinomadura melliaura_atS8(atmS8) Putative UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase Actinomadura melliauraにおけるAT2433のaminodideoxypentose生合成に関連する・dTDP-α-D-glucose dehydrogenase (AtmS8) ・dTDP-α-D-glucuronic acid decarboxylase (AtmS9) ・dTDP-4-keto-α-D-xylose 2,3-dehydratase (AtmS14) の特徴づけ。 AT2433とcalicheamicinに共通するaminodeoxypentose経路が提唱されている。

Sequence

>putative NDP-alpha-D-glucose dehydrogenase [NDP glucose dehydrogenase]
MPDNQPDPGTVAVVGLGYVGSCLAAALAERGLNVLGIDTDEQLIDEYNAGFCRFREDMLA
DGIARALAAGRLHLTTDHRAVSRAGVVLIAVGTPVHADGSLADGQLRAACGALGEHLRPG
QLAVLKSTVPPGTTRELVRPLLEQGGLRAGRDFRLAFSPERLAEGTALTELRSFPIVVGG
IDARSTAEAAEFWRRGLGVATVPQESLEAAELVKLADNWWIDLNIALANELARLCALYGA
DVLDVIEAANTVPKGNGHVNILLPGVGVGGSCLTKDPWMVWHAARLRGLELHTPRAGRAA
NSAMPEYTAQLIIDDLLECGKDPAESTVAVLGLAFKNDTGDLRATPTQGVIAALTKAGVR
VRCHDPLVDADAAEDLLNVRPLPTVEETVRDADCLAVLAHHRAFDAIEYAALEPAGSCLI
LDGRDHFPKDKVAKLRELGYRYRGIGR

>putative NDP-alpha-D-glucose dehydrogenase [NDP glucose dehydrogenase]
GTGCCGGACAACCAGCCGGACCCGGGGACCGTCGCGGTCGTCGGGCTCGGGTACGTGGGG
TCGTGCCTCGCGGCGGCCCTGGCCGAGCGCGGCCTGAACGTCCTCGGGATCGACACCGAC
GAGCAGCTCATCGACGAGTACAACGCGGGCTTCTGCAGGTTCCGCGAGGACATGCTGGCG
GACGGCATCGCCCGCGCGCTGGCGGCGGGCAGGCTGCACCTGACCACCGACCACCGGGCC
GTGTCCCGGGCCGGCGTGGTGCTGATCGCCGTCGGCACGCCCGTCCACGCCGACGGGTCG
CTGGCCGACGGGCAGCTCCGCGCCGCCTGCGGGGCGCTCGGCGAGCACCTGCGCCCCGGC
CAGCTCGCGGTGCTCAAGAGCACCGTCCCGCCGGGCACCACCCGGGAGCTGGTGCGGCCG
CTGCTGGAGCAGGGCGGCCTGCGGGCGGGCCGGGACTTCCGGCTGGCCTTCAGCCCGGAA
CGGCTCGCGGAGGGGACGGCGCTCACCGAACTGCGCTCGTTCCCCATCGTGGTCGGCGGA
ATCGACGCGCGCAGCACCGCGGAGGCGGCGGAGTTCTGGCGGCGGGGCCTGGGCGTGGCG
ACGGTCCCGCAGGAGTCGCTGGAGGCCGCGGAGCTGGTCAAGCTCGCCGACAACTGGTGG
ATCGACCTGAACATCGCCCTCGCCAACGAGCTGGCCAGGCTGTGCGCGCTGTACGGCGCG
GACGTCCTGGACGTGATCGAGGCGGCCAACACCGTCCCCAAGGGCAACGGCCACGTCAAC
ATCCTGCTGCCCGGCGTCGGGGTCGGGGGGTCGTGCCTGACCAAGGACCCGTGGATGGTG
TGGCACGCGGCCCGCCTGCGCGGGCTGGAGCTGCACACGCCCCGTGCCGGCCGCGCGGCC
AACTCGGCGATGCCGGAGTACACCGCGCAGCTGATCATCGACGACCTGCTCGAGTGCGGC
AAGGACCCCGCCGAGTCCACGGTCGCGGTGCTGGGGCTGGCGTTCAAGAACGACACCGGG
GACCTGCGCGCCACCCCGACCCAGGGCGTCATCGCGGCCCTGACGAAGGCCGGGGTGCGG
GTGCGCTGCCACGATCCGCTGGTCGACGCCGACGCGGCCGAGGACCTGCTGAACGTCCGG
CCGCTGCCCACGGTGGAGGAGACCGTCCGGGACGCCGACTGCCTGGCGGTCCTGGCCCAC
CACCGCGCGTTCGACGCGATCGAGTACGCCGCGCTGGAGCCGGCCGGGTCGTGCCTGATC
CTGGACGGCCGCGACCACTTCCCGAAGGACAAGGTCGCGAAGCTCCGCGAGCTCGGCTAC
CGCTACCGCGGAATCGGGAGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001732 UDP-glucose/GDP-mannose dehydrogenase, N-terminal (Domain) PF03721 UDPG_MGDP_dh_N IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain) SSF48179 6DGDH_C_like IPR014026 UDP-glucose/GDP-mannose dehydrogenase, dimerisation (Domain) PF00984 UDPG_MGDP_dh IPR014027 UDP-glucose/GDP-mannose dehydrogenase, C-terminal (Domain) SSF52413 UDP-Glc/GDP-Man_DH_C SM00984 UDPG_MGDP_dh_C PF03720 UDPG_MGDP_dh_C IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR017476 Nucleotide sugar dehydrogenase (Family) PIRSF000124 UDPglc_GDPman_dh TIGR03026 NDP-sugDHase
SignalP	No significant hit
TMHMM	No significant hit

Madu_00110 Madu_00130