Madu_00190 Madu_00190   Madu_00210 Madu_00210

Madu_00200 : CDS information

close this sectionLocation

Organism
StrainATCC 39144
Entry nameMaduropeptin
Contig
Start / Stop / Direction34,898 / 33,498 / - [in whole cluster]
34,898 / 33,498 / - [in contig]
Locationcomplement(33498..34898) [in whole cluster]
complement(33498..34898) [in contig]
TypeCDS
Length1,401 bp (466 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glycosyltransferase
Product (GenBank)glycosyltransferase
Gene
Gene (GenBank)mdpA6
EC number
Keyword
  • madurose
Note
Note (GenBank)
Reference
ACC
PmId
[17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007)
comment
maduropeptinの生合成クラスタ-論文

mdpA6: Glycosyltransferase

mdpA6, mdpB3, mdpC5は、4つのbuilding blocksをmaduropeptin chromophoreへと最終的に共有結合させるのに関与するとしている。aminosugar maduroseはglycosyltransferase MdpA6を介して付着される。

機能解析はしていない。
Related Reference
ACC
Q9RPA2
NITE
Urd_00030
PmId
[10662691] Function of glycosyltransferase genes involved in urdamycin A biosynthesis. (Chem Biol. , 2000)
[11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000)
comment
Streptomyces fradiae_urdGT1a
Glycosyl transferase

UniProtで登録されている[PMID:10658661]にurdGT1aについての記載はない。

--
[PMID:10662691]
[PMID:11094336]

urdamycin clusterに含まれるglycosyltransferase候補genes=urdGT1a, urdGT1b, urdGT1cについて、mutant作成、相補実験における蓄積産物解析を行い、各機能割り当てが確定されている。

UrdGT1aは、urdamycin A生合成においてC-12bのhydroxy基にL-rhodinoseを付着するO-glycosyltransferaseである。

C-9にあるtrisaccharideでもL-rhodinoseを付着するglycosyltransferase(UrdGT1c)が作用するが、UrdGT1aはこれを代替しない。
ACC
Q9L555
NITE
Acla_00030
PmId
[15078101] AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A. (Biochemistry. , 2004)
[17685523] Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A. (J Am Chem Soc. , 2007)
comment
Streptomyces galilaeus_aknK
AknK
[DoBISCUIT]L-2-deoxyfucose glycosyltransferase

--
[PMID: 15078101] abstract
aclacinomycin生合成遺伝子
AknKを異種性発現して精製、実験に使用。
dTDP-L-2-deoxyfucoseとrhodosaminyl aklavinoneから、AknKはL-2-deoxyfucosyl-L-rhodosaminyl aklavinoneを生成。
dTDP-L-daunosamineなどでも作用し、anthracycline骨格で2糖目を組み立てる。
活性は下がるが、rhodosaminyl aklavinoneにdTDP-L-2-deoxyfucoseをtandemに追加して、L-deoxyfucosyl-L-deoxyfucosyl-L-rhodosaminyl aklavinoneを産生することもできる。

--
[PMID: 17685523]
AknSはゆっくりな基質としてdTDP-2-deoxyfucoseを受け入れるけど、AknKはdTDP-L-rhodosamineを利用できない。

close this sectionSequence

selected fasta
>putative glycosyltransferase [glycosyltransferase]
MRVLFVVYPSHAHLWPAVPTAWALQSAGHEVRVATHANFSASVTAAGLTPVQLGDADAVE
ARMRPDAKPPARPEEVLHFADVLGLNERERENWIPYYQWLLNPISDYVRTDLPEAGDLID
FACAWRPDLIVWDPTFASGPVAARLCGAAHARMVLGPDYLSWSFDRLAERRDAVRAAGLP
ESPQHELLRPLAEKHGVELDDELLYGQWSLDPMPPGMRLPTSGITVPMRYVPYNGAEIMP
KWLYERPSRPRVALTLGESTRRFIKGDWGRTPKIFEAVAGLDIEVVATLNDLQMEGIERL
PDNVRAIEWVPLTHLLPTCDVMINHGGIGTTAAAWGLRTPQIVCDTDDTLMMRPVEVDPR
TMEDGTYRVGFEFGVREEVEMVTTWELPPKKLEAGPTSDYIVSRGAGARLDHTKLSVAEV
ADVISAVVEDPAYRKGAQDVYDEWLGLPSPAGIVPQLEQLTAEHRR
selected fasta
>putative glycosyltransferase [glycosyltransferase]
ATGCGGGTCCTGTTCGTCGTCTACCCTTCCCACGCGCATCTCTGGCCGGCCGTGCCGACC
GCGTGGGCATTGCAAAGTGCCGGGCACGAGGTCCGTGTCGCCACCCATGCGAATTTCAGC
GCGTCGGTCACGGCGGCGGGACTGACCCCCGTCCAGCTCGGTGACGCCGACGCGGTCGAG
GCGCGCATGCGGCCCGACGCCAAGCCGCCCGCGCGGCCCGAGGAGGTGCTCCACTTCGCG
GACGTGCTCGGGCTTAACGAGCGGGAGCGCGAGAACTGGATCCCCTACTACCAGTGGCTG
CTGAACCCGATCTCGGACTACGTCCGCACGGACCTGCCCGAGGCGGGCGACCTCATCGAC
TTCGCGTGCGCCTGGCGGCCCGACCTGATCGTCTGGGACCCCACCTTCGCGTCCGGTCCC
GTGGCGGCACGGCTCTGCGGCGCCGCGCACGCCCGCATGGTGCTGGGCCCCGACTACCTC
TCGTGGAGCTTCGACCGGCTCGCCGAGCGCCGCGACGCGGTCCGGGCCGCGGGCCTGCCC
GAGAGCCCCCAGCACGAGCTGCTGCGCCCGCTCGCCGAGAAGCACGGCGTCGAGCTCGAC
GACGAGCTGCTGTACGGCCAGTGGTCGCTGGACCCCATGCCGCCGGGCATGCGGCTGCCG
ACGAGCGGGATCACGGTGCCGATGCGCTACGTGCCGTACAACGGCGCCGAGATCATGCCG
AAGTGGCTGTACGAGCGGCCCAGCCGCCCGCGGGTCGCGCTGACGCTCGGTGAGTCCACC
CGCCGGTTCATCAAGGGCGACTGGGGCCGCACCCCGAAGATCTTCGAGGCGGTGGCCGGT
CTCGACATCGAGGTGGTCGCCACGCTCAACGACCTGCAGATGGAGGGGATCGAGCGGCTG
CCCGACAACGTCCGGGCGATCGAATGGGTGCCGCTGACCCACCTCCTGCCCACCTGCGAC
GTGATGATCAACCACGGCGGCATCGGGACGACCGCCGCGGCCTGGGGGCTGCGGACGCCG
CAGATCGTCTGCGACACCGACGACACCCTGATGATGCGTCCCGTCGAGGTGGACCCCCGG
ACCATGGAGGACGGCACGTACCGCGTCGGCTTCGAGTTCGGCGTCCGGGAGGAGGTCGAG
ATGGTCACCACCTGGGAGCTGCCGCCCAAGAAGCTGGAGGCCGGCCCGACGTCGGACTAC
ATCGTGTCGCGCGGCGCGGGCGCCCGGCTGGACCACACGAAGCTGTCGGTCGCCGAGGTC
GCCGACGTGATCAGCGCCGTGGTGGAGGACCCGGCGTACCGCAAGGGCGCGCAGGACGTC
TACGACGAGTGGCTCGGCCTCCCGAGCCCGGCCGGCATCGTGCCGCAGCTGGAGCAGCTG
ACGGCAGAGCACCGGCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-72] 2.6e-12 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[210-305] 7e-32 PF06722
PF06722   DUF1205
SignalP No significant hit
TMHMM No significant hit
Madu_00190 Madu_00190   Madu_00210 Madu_00210
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