Madu_00300 Madu_00300   Madu_00320 Madu_00320

Madu_00310 : CDS information

close this sectionLocation

Organism
StrainATCC 39144
Entry nameMaduropeptin
Contig
Start / Stop / Direction50,251 / 48,638 / - [in whole cluster]
50,251 / 48,638 / - [in contig]
Locationcomplement(48638..50251) [in whole cluster]
complement(48638..50251) [in contig]
TypeCDS
Length1,614 bp (537 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Product4-methylideneimidazole-5-one(MIO)-dependent tyrosine aminomutase
Product (GenBank)tyrosine aminomutase
Gene
Gene (GenBank)mdpC4
EC number
Keyword
  • (S)-3-(2-chloro-3-hydroxy-4-methoxyphenyl)-3-hydroxypropionic acid
Note
Note (GenBank)
Reference
ACC
PmId
[17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007)
comment
Maduropeptin生合成遺伝子クラスターの報告

mdpC4: Aminomutase

Eight genes (mdpC to mdpC8 excluding mdpC5)は、L-alpha-tyrosineからの(S)-3-(2-chloro-3-hydroxy-4-methoxyphenyl)-3-hydroxypropionic acid moietyの生合成に関与するとしている。


mdpC4,mdpC1を大腸菌でcloning、S.lividansでsubcloningしている。それぞれ精製し、活性を測定している。mdpC4はSgcC4と同様に、(S)-beta-tyrosine:L-alpha-tyrosine=7:3で可逆反応を行うことを示した。mdpC4は全てのhypothetic alpha-amino acid(L-4-methoxyphenylalanine, L-3-chlorotyrosine, and L-3-hydroxytyrosine等)を基質として認識しなかった。

MdpC4はMIO-containing aminomutasesのfamilyで、外因的なcofactorを使うことなくL-alpha-tyrosineを(S)-beta-tyrosineへ触媒する酵素だと結論付けている。

close this sectionSequence

selected fasta
>4-methylideneimidazole-5-one(MIO)-dependent tyrosine aminomutase [tyrosine aminomutase]
MKVTQTEIRMPVDINGEDMDIDGLRQVAEQRIPCRLTPTVLDKAAKSRAQFETLVTQDVP
VYGVTTGYGEMIYMLVDRANEVELQTNLVRSHSAGVGRPFAEDESRAIVAARLNALAKGH
SAVRPEILERLALYLNLGITPAIPEIGSLGASGDLAPLSHLACTLIGEGRILRDGAAVET
GPELRRLGIEPLELRFKEGLALINGTSAMTGLGALVVGRAFEQIRQAEICTALVIEALRG
SMGPFQPEGHDIARPHPGQIDSAANMRALLRGSGLTVEHADLRRTVQERKREEESVQRTD
IYMQKAYSLRAIPQVLGAVRDTLGRAGETVEVELNSATDNPLFFEGREIFHGANFHGQPV
AFSMDFTTIALTQLGVLSERRLNRLLNRHLNYGLPEFLVMGEPGLNSGFAGAQYPATALV
AENRTIGPASTQSIPSNGDNQDIVSMGLIGARNARRVLNNNHTILALEFLAAAQAVDVAG
RRDGLGPAGAATYELVRSMAPTLERDRYMADEIEAVADAVSRGAFLEAARSAGVELR
selected fasta
>4-methylideneimidazole-5-one(MIO)-dependent tyrosine aminomutase [tyrosine aminomutase]
GTGAAGGTGACTCAGACCGAGATTCGAATGCCGGTTGACATCAACGGCGAGGACATGGAC
ATCGACGGGCTCCGGCAGGTGGCCGAGCAGCGGATCCCGTGCCGGCTGACGCCGACGGTG
CTGGACAAGGCCGCCAAGAGCCGCGCGCAGTTCGAGACCCTCGTCACGCAGGACGTCCCC
GTCTACGGGGTCACCACCGGCTACGGCGAGATGATCTACATGCTGGTGGACCGGGCCAAC
GAGGTGGAACTGCAGACCAACCTGGTGCGCAGCCACAGCGCCGGGGTCGGCCGGCCGTTC
GCCGAGGACGAGTCGCGCGCGATCGTCGCCGCCCGGCTCAACGCGCTGGCCAAGGGCCAC
TCGGCCGTCCGGCCCGAGATCCTGGAGCGGCTCGCGCTCTACCTCAACCTGGGCATCACC
CCGGCCATCCCCGAGATCGGCTCGCTGGGCGCCAGCGGCGACCTGGCGCCCCTGTCGCAT
CTGGCGTGCACGCTCATCGGCGAGGGCCGGATCCTGCGGGACGGCGCGGCGGTGGAGACC
GGGCCGGAGCTGCGCCGGCTGGGCATCGAGCCGCTGGAGCTGCGCTTCAAGGAGGGCCTC
GCGCTGATCAACGGCACGTCCGCGATGACCGGGCTGGGGGCGCTGGTCGTCGGCCGCGCG
TTCGAGCAGATCCGGCAGGCCGAGATCTGCACCGCGCTGGTCATCGAGGCGCTGCGCGGG
TCGATGGGCCCGTTCCAGCCGGAGGGGCACGACATCGCCCGTCCGCACCCCGGGCAGATC
GACTCGGCGGCCAACATGCGCGCCCTGCTGCGCGGCAGCGGCCTGACCGTCGAGCACGCC
GACCTGCGCCGCACCGTCCAGGAGCGCAAGCGGGAGGAGGAGAGCGTCCAGCGCACCGAC
ATCTACATGCAGAAGGCGTACTCGCTGCGGGCGATCCCGCAGGTGCTCGGCGCCGTCCGC
GACACGCTCGGCAGGGCCGGCGAGACGGTCGAGGTCGAGCTGAACTCCGCCACCGACAAC
CCGCTGTTCTTCGAGGGCCGGGAGATCTTCCACGGCGCCAACTTCCACGGCCAGCCGGTC
GCGTTCTCCATGGACTTCACGACGATCGCGCTGACCCAGCTCGGCGTCCTGTCGGAGCGG
CGCCTCAACCGCCTGCTCAACCGGCACCTCAACTACGGCCTGCCGGAGTTCCTGGTCATG
GGCGAGCCCGGCCTCAACAGCGGCTTCGCGGGCGCGCAGTACCCGGCGACCGCGCTGGTC
GCCGAGAACCGGACGATCGGCCCGGCCAGCACGCAGTCCATCCCGTCCAACGGCGACAAC
CAGGACATCGTGAGCATGGGGCTGATCGGCGCCCGCAACGCGCGCCGCGTCCTGAACAAC
AACCACACGATCCTGGCCCTGGAGTTCCTCGCCGCCGCCCAGGCGGTGGACGTCGCCGGA
CGCCGCGACGGGCTCGGCCCCGCGGGCGCCGCGACGTACGAGCTCGTCCGGTCGATGGCG
CCGACCCTGGAACGCGACCGGTACATGGCCGACGAGATCGAGGCCGTGGCCGACGCGGTC
TCGCGCGGGGCCTTCCTGGAGGCGGCCCGCTCCGCGGGCGTCGAGCTGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001106 Aromatic amino acid lyase (Family)
[12-504] PF00221
PF00221   Lyase_aromatic
IPR008948 L-Aspartase-like (Domain)
[11-536] SSF48557
SSF48557   L-Aspartase-like
IPR022313 Phenylalanine/histidine ammonia-lyases, active site (Active_site)
[147-162] PS00488
PS00488   PAL_HISTIDASE
IPR022314 Tyrosine 2,3-aminomutase, putative (Family)
[14-520] TIGR03832
TIGR03832   Tyr_2_3_mutase
IPR024083 L-Aspartase-like, N-terminal (Domain)
[4-205] 4.2e-65 G3DSA:1.10.275.10
G3DSA:1.10.275.10   G3DSA:1.10.275.10
SignalP No significant hit
TMHMM No significant hit
Madu_00300 Madu_00300   Madu_00320 Madu_00320
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