Madu_00410 Madu_00410   Madu_00430 Madu_00430

Madu_00420 : CDS information

close this sectionLocation

Organism
StrainATCC 39144
Entry nameMaduropeptin
Contig
Start / Stop / Direction63,617 / 63,066 / - [in whole cluster]
63,617 / 63,066 / - [in contig]
Locationcomplement(63066..63617) [in whole cluster]
complement(63066..63617) [in contig]
TypeCDS
Length552 bp (183 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative NADH-dependent FAD reductase
Product (GenBank)flavin reductase
Gene
Gene (GenBank)mdpE6
EC number
Keyword
  • two-component system
  • (S)-3-(2-chloro-3-hydroxy-4-methoxyphenyl)-3-hydroxypropionic acid
Note
Note (GenBank)
Reference
ACC
PmId
[17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007)
comment
Maduropeptin生合成遺伝子クラスターの報告

mdpM: Flavin reductase

12ORFs(MdpE through MdpE11)がenediyne coreの生合成に関与するとしている。
機能解析はされていない。
Related Reference
ACC
Q8GME2
NITE
C1027_00510
PmId
[19817865] Characterization of SgcE6, the flavin reductase component supporting FAD-dependent halogenation and hydroxylation in the biosynthesis of the enediyne antitumor antibiotic C-1027. (FEMS Microbiol Lett. , 2009)
comment
Streptomyces globisporus
Oxidoreductase
C-1027生合成遺伝子

SgcE6の機能解析
Sequence alignmentは、SgcE6がClass I flavin reductasesのHpaC-like subfamilyであることを示した。
SgcE6 は基質として唯一NADHとFADを認識した。FAD and NADH substratesに対するカイネティクス解析は、 flavin reductase enzymesのタイプのKm valuesを示した。

SgcE6は、flavin-dependent halogenase SgcC3 and monooxygenase SgcCへreduced flavinを提供することができるflavin reductaseだとしている。

close this sectionSequence

selected fasta
>putative NADH-dependent FAD reductase [flavin reductase]
MSVDPGQSPGEVGADMTATDLRRAFGTFATGVTVVTVGGREPHGMTANSFTSVSLDPPLV
LVCVGRSAVMHQRLAVGSFGISVLGAGQESVARYFADLSRPLGAAQFDRIGCTPGRATGA
PLIDGALAHYECELWSTAEAGDHTIFVGRLLTVHRAEPGPGEGLLFYQGRFRRLEPGRSE
VKA
selected fasta
>putative NADH-dependent FAD reductase [flavin reductase]
ATGAGCGTCGATCCCGGTCAGTCCCCGGGCGAGGTCGGCGCGGACATGACGGCCACCGAC
CTGCGCCGGGCCTTCGGGACGTTCGCCACGGGCGTCACCGTGGTCACCGTCGGCGGCCGC
GAGCCGCACGGCATGACCGCGAACTCCTTCACCTCGGTCTCGCTCGACCCGCCGCTGGTG
CTGGTCTGCGTCGGGCGCAGCGCGGTCATGCACCAGCGGCTGGCCGTCGGCTCCTTCGGC
ATCTCGGTGCTGGGGGCCGGGCAGGAGTCGGTGGCGCGGTACTTCGCGGACCTGTCCCGC
CCGCTGGGCGCGGCCCAGTTCGACCGGATCGGCTGCACGCCGGGCCGGGCCACCGGGGCG
CCGCTGATCGACGGCGCGCTCGCGCACTACGAGTGCGAGCTGTGGAGCACCGCCGAGGCG
GGGGACCACACGATCTTCGTCGGGCGGCTGCTGACGGTGCACCGCGCCGAGCCGGGGCCC
GGCGAGGGGCTGCTGTTCTACCAGGGCCGCTTCCGGCGGCTGGAGCCCGGACGGAGCGAG
GTGAAGGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002563 Flavin reductase-like, FMN-binding (Domain)
[25-173] 1.79999754022375e-55 SM00903
SM00903   Flavin_Reduct
[26-174] 4.39999999999997e-36 PF01613
PF01613   Flavin_Reduct
IPR009002 FMN-binding split barrel-related (Domain)
[13-182] 5.8999880940569e-47 SSF50475
SSF50475   FMN_binding
IPR012349 FMN-binding split barrel (Domain)
[16-176] 2.4e-52 G3DSA:2.30.110.10
G3DSA:2.30.110.10   PNPOx_FMN_bd
SignalP No significant hit
TMHMM No significant hit
Madu_00410 Madu_00410   Madu_00430 Madu_00430
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