Madu_00400 Madu_00400   Madu_00420 Madu_00420

Madu_00410 : CDS information

close this sectionLocation

Organism
StrainATCC 39144
Entry nameMaduropeptin
Contig
Start / Stop / Direction63,069 / 61,675 / - [in whole cluster]
63,069 / 61,675 / - [in contig]
Locationcomplement(61675..63069) [in whole cluster]
complement(61675..63069) [in contig]
TypeCDS
Length1,395 bp (464 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)putative P450 hydroxylase
Gene
Gene (GenBank)mdpE7
EC number
Keyword
Note
Note (GenBank)
  • MadE7
Reference
ACC
PmId
[17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007)
comment
Maduropeptin生合成遺伝子クラスターの報告

mdpE7: P-450 hydroxylase

12ORFs(MdpE through MdpE11)がenediyne coreの生合成に関与するとしている。
機能解析はされていない。
Related Reference
ACC
O23365
PmId
[19939422] Over-expression of Arabidopsis thaliana carotenoid hydroxylases individually and in combination with a beta-carotene ketolase provides insight into in vivo functions. (Phytochemistry. , 2010)
comment
Arabidopsis thaliana_CYP97B3
Cytochrome P450 97B3
27%id 2e-46

ferredoxin-dependent non-heme di-iron monooxygenases(AtB1)とheme-containing cytochrome P450(CYP) monooxygenases(CYP97A3 and CYP97C1) とhydroxylase候補(CYP97B3)の過剰発現株を作製。alpha-caroteneとbeta-caroteneのbeta-, epsilon-rings の修飾におけるhydroxylase classesの役割を調べた。
foliar carotenoid profiles分析は、CYP97B3がcarotenoid biosynthesisに関与することを示した。そしてknock-out mutantsの解析によりhydroxylase activitiesを確かめた。carotenoid biosynthesisにおいて、multifunctional enzymesとして機能すると示唆された。
Family/Domain
FD
IPR001128
comment
[IPR001128] Cytochrome P450 (Family)

close this sectionSequence

selected fasta
>putative cytochrome P450 [putative P450 hydroxylase]
MSIDELDARGGTPRAAGRVPPGPPRRATPNLLRMLATDRLGMMQAALRHGDAVRVGLGPK
ALYLFNRPEHAKHVLADNSGNYHKGIGLVQARRALGDGLLTSEGDLWREQRRVVQPAFQH
KRIAGLADAVVEEAGALVARLRARAGGPPVDVVGEMTALTLGVLGRTLLDADLTAHTSLG
RAFETVQDQAMFEMVSQGMVPMWLPLPGQLRFRRARRELDRIVRALVAERLREGGGAEDA
LSRLIESARREPDGRVGRRRLRDELVTLLLAGHETTASTLGWTFHLLDRHPLVRARVRAE
ARAVFGDGTPTLDDLSALSYTTMVVQEVMRMYPPVWILTRVAQRDDEVGGYRVPAGADVL
VCPYTLHRNPDFWEWPDVFDPDRFGPGRSEGRPRYAYIPFGAGPRFCVGNHLGMMEAVFV
TAMVARDLRLTGVPGRDAVPEPMLSLRVRGGLPMTVHDDAGAGA
selected fasta
>putative cytochrome P450 [putative P450 hydroxylase]
GTGAGCATCGACGAGCTGGACGCCCGCGGCGGGACGCCGCGGGCCGCCGGCCGGGTCCCG
CCGGGACCGCCCCGCCGCGCGACGCCGAACCTGCTGCGGATGCTGGCGACCGACCGGCTC
GGCATGATGCAGGCGGCCCTCCGGCACGGCGACGCCGTCCGGGTCGGGCTCGGGCCCAAG
GCCCTGTACCTGTTCAACCGTCCCGAGCACGCCAAGCACGTCCTGGCGGACAACAGCGGG
AACTACCACAAGGGCATCGGCCTGGTGCAGGCCCGGCGCGCCCTCGGTGACGGGCTCCTG
ACCAGCGAGGGCGACCTGTGGCGCGAGCAGCGCAGGGTCGTCCAGCCCGCGTTCCAGCAC
AAGCGGATCGCCGGGCTGGCCGACGCGGTCGTCGAGGAGGCGGGCGCGCTGGTCGCCCGG
CTCCGGGCCCGCGCGGGCGGCCCCCCGGTCGACGTGGTGGGGGAGATGACCGCGCTGACC
CTCGGGGTGCTCGGACGCACTCTGCTGGACGCCGACCTGACCGCGCACACCTCCCTGGGA
CGGGCGTTCGAGACCGTCCAGGACCAGGCGATGTTCGAGATGGTGAGCCAGGGCATGGTT
CCGATGTGGCTCCCGCTGCCCGGCCAGCTCCGCTTCCGCCGGGCCCGCCGGGAGCTGGAC
CGCATCGTCCGCGCCCTGGTGGCCGAGCGCCTCCGCGAGGGCGGCGGCGCCGAGGACGCG
CTGTCCCGGCTGATCGAGTCGGCGCGGCGGGAACCGGACGGCCGGGTCGGGCGGCGGCGG
CTGCGCGACGAACTCGTCACGCTGCTGCTGGCCGGGCACGAGACGACCGCGAGCACGCTC
GGCTGGACGTTCCACCTGCTCGACCGGCACCCGCTGGTGCGGGCCCGGGTGCGCGCGGAG
GCACGGGCGGTCTTCGGCGACGGGACGCCCACCCTGGACGACCTCTCCGCGCTGTCGTAC
ACCACGATGGTCGTGCAGGAGGTCATGCGGATGTACCCGCCCGTGTGGATCCTCACGCGG
GTGGCGCAGCGGGACGACGAGGTCGGCGGGTACCGCGTCCCGGCGGGCGCCGACGTGCTC
GTCTGCCCCTACACGTTGCACCGTAACCCGGATTTCTGGGAATGGCCCGATGTGTTCGAC
CCCGATCGCTTCGGTCCCGGGCGCTCCGAGGGCCGGCCGCGCTACGCCTACATCCCGTTC
GGGGCCGGTCCGCGCTTCTGCGTCGGCAACCACCTCGGCATGATGGAGGCCGTCTTCGTG
ACGGCCATGGTGGCGCGCGACCTGCGGCTGACCGGGGTGCCCGGCCGCGACGCCGTGCCC
GAGCCGATGCTGTCCCTGCGGGTGCGCGGGGGCCTGCCGATGACGGTGCACGACGACGCC
GGCGCCGGCGCCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[271-288] 3.0999994589937e-13 PR00385 [323-334] 3.0999994589937e-13 PR00385 [398-407] 3.0999994589937e-13 PR00385 [407-418] 3.0999994589937e-13 PR00385
PR00385   P450
[20-458] 3.699978135861e-110 SSF48264
SSF48264   Cytochrome_P450
[42-440] 1.69999999999998e-77 PF00067
PF00067   p450
[31-457] 2.59999999999995e-111 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002401 Cytochrome P450, E-class, group I (Family)
[46-65] 2.60000517657733e-19 PR00463 [260-277] 2.60000517657733e-19 PR00463 [280-306] 2.60000517657733e-19 PR00463 [362-386] 2.60000517657733e-19 PR00463 [397-407] 2.60000517657733e-19 PR00463 [407-430] 2.60000517657733e-19 PR00463
PR00463   EP450I
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[400-409] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Madu_00400 Madu_00400   Madu_00420 Madu_00420
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