Madu_00450 Madu_00450   Madu_00470 Madu_00470

Madu_00460 : CDS information

close this sectionLocation

Organism
StrainATCC 39144
Entry nameMaduropeptin
Contig
Start / Stop / Direction72,943 / 70,907 / - [in whole cluster]
72,943 / 70,907 / - [in contig]
Locationcomplement(70907..72943) [in whole cluster]
complement(70907..72943) [in contig]
TypeCDS
Length2,037 bp (678 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Producthypothetical protein
Product (GenBank)unknown
GenemdpE4
unbV
Gene (GenBank)mdpE4
EC number
Keyword
  • enediyne core
Note
Note (GenBank)
  • MadE4
Reference
ACC
PmId
[17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007)
[12536216] A genomics-guided approach for discovering and expressing cryptic metabolic pathways. (Nat Biotechnol. , 2003)
comment
[PMID:17918933]
Maduropeptin生合成遺伝子クラスターの報告

mdpE4: Unknown

12のORFs(MdpE through MdpE11)はenediyne core生合成に関与すると言っている。
機能解析はされていない。


[PMID:12536216]
warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)の報告。

warhead cassette(UNBL-UNBV-UNBU-PKSE-TEBC)はactinomyceteにおいて全てのenediyne生合成遺伝子座で保存されているが、機能は未知のままである。enediyne warheadのformation, stabilization, or transport に関与しているのではないかと推測している。

UNBVの構造解析は、N末端シグナル配列と共にsecreted proteinを持っていた。
MaduropeptinでもMadu_00430-Madu_00470で保存されている。
Related Reference
ACC
Q84HJ0
NITE
Dynm_00160
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
comment
Micromonospora chersina_dynU14
DynU14
dynemicin生合成遺伝子

dynE8, orf8, dynU15, dynU14 and orf23の欠損株を作製し、培養液中の産物をHPLCにて検出。orf8はdynemicin Aが検出できたが、dynE8, dynU15, dynU14，orf23の欠損株はdynemicin Aが検出できなかった。ただし、orf23の欠損株はdynemicin Aの新規化合物が検出された。
よって、dynE8, dynU15, dynU14はdynemicin Aに必須であることを示した。

dynU15, U14, T3,E8, E7 は、minimal enediyne cassetteとして知られている遺伝子。
DynU15, U14, T3はconserved unknown proteins。

close this sectionSequence

selected fasta
>hypothetical protein [unknown]
MSASYGWLRKQLPGVVALVLVVGAFFVVKLPEAPAAEKADVAARYGFEPMSIAMPGGFAQ
QTIREVNKDYENIDAWISSVGAGVAMNDLDGDGLPNDLCITDPRIDRVVVTPAPGARSDR
YASFALETGPLPMNDAMAPMGCAPGDFNEDGRMDLLVYMWGRTPIVHLARADAGKLTADA
YVPTELVPNAGGRAYTGPQWNSNAVAIDDFDGDGHYDIYLGNYFPHGPVLDPRKSGGVEM
NRSLSNATNAGEDYFFRWTGATAGERPSVEFEQLDDVLPREISKGWVLAAGANDLDGDQR
PELYIAQDHGKDALLYNISTPGKIEFQAVHGARTPGLPKSLRIGDDSFKGMGIDFADFDH
DGLYDMFVSNITTTFGIQESNLHFYNTAKNQADLRTRLRDGDAPWTDRSADKGTAWSGWA
WDVKVGDFDNSGDPAIAQTNGFVKGEVNRWPQLQELAASNDLVVEDPRWWPHVRAGDDIA
GSQRLTFWAPDDDGGYVNVSDELGLAIPVPTRGIAVGDADGDGRLDMAVARQWEEPVFYH
NTAPSPGAHLNLRLTHDTEDGDSGSGGSEPAEGALPAPGSPVVGAQVKVTTPDGRAHLAR
VDGGSGHSGKRSDEVHIGLGAGVTGPVRVELCWRDRSGTEHRQELRLAPGRHSLRLGAGA
EVVRPDQPQDTQTPAEEE
selected fasta
>hypothetical protein [unknown]
GTGTCCGCGAGCTATGGCTGGCTGCGCAAGCAGCTACCGGGCGTTGTGGCGCTCGTACTG
GTGGTCGGGGCGTTCTTCGTGGTCAAGCTGCCCGAGGCGCCCGCCGCCGAGAAGGCCGAT
GTGGCGGCCCGGTACGGGTTCGAGCCGATGAGCATCGCGATGCCGGGCGGCTTCGCGCAG
CAGACGATCCGCGAGGTCAACAAGGACTACGAGAACATCGACGCCTGGATCTCCTCCGTC
GGAGCCGGCGTCGCGATGAACGACCTCGACGGCGACGGGCTGCCCAACGACCTGTGCATC
ACCGATCCGCGGATCGACAGGGTCGTGGTGACCCCGGCTCCGGGCGCCCGCTCGGACCGG
TACGCGTCGTTCGCGCTGGAGACCGGCCCGCTGCCGATGAACGACGCGATGGCCCCGATG
GGGTGCGCCCCCGGCGACTTCAACGAGGACGGCCGGATGGACCTGCTGGTCTACATGTGG
GGCCGCACCCCGATCGTCCATCTCGCCCGCGCGGACGCCGGGAAGCTGACGGCGGACGCG
TACGTCCCGACCGAGCTGGTGCCGAACGCGGGCGGCCGGGCCTACACGGGGCCGCAGTGG
AACTCCAACGCCGTGGCGATCGACGACTTCGACGGCGACGGCCACTACGACATCTACCTG
GGGAACTACTTCCCGCACGGTCCCGTGCTCGATCCCCGCAAGAGCGGCGGCGTCGAGATG
AACCGGTCGCTGTCGAACGCGACCAACGCCGGGGAGGACTACTTCTTCCGCTGGACGGGC
GCCACCGCCGGTGAACGTCCGTCGGTCGAGTTCGAGCAGCTCGACGACGTGCTCCCGCGC
GAGATCTCCAAGGGCTGGGTGCTGGCGGCCGGGGCGAACGACCTCGACGGCGACCAGCGC
CCCGAGCTCTACATCGCCCAGGACCACGGCAAGGACGCCCTCCTCTACAACATTTCCACT
CCCGGAAAGATCGAATTCCAGGCCGTGCACGGGGCCCGCACTCCGGGCCTGCCGAAGTCG
CTGCGGATCGGCGACGATTCCTTCAAGGGTATGGGAATCGATTTCGCCGATTTCGATCAC
GACGGCCTGTACGACATGTTCGTCAGCAACATCACGACGACGTTCGGCATCCAGGAGAGC
AATCTCCATTTCTACAACACCGCGAAGAACCAGGCCGATCTCCGGACCAGGCTGCGTGAC
GGAGACGCGCCGTGGACGGACCGCAGCGCCGACAAGGGCACCGCGTGGTCCGGCTGGGCC
TGGGACGTCAAGGTGGGCGACTTCGACAACAGCGGCGACCCCGCCATCGCCCAGACCAAC
GGGTTCGTCAAGGGCGAGGTGAACCGGTGGCCCCAGCTGCAGGAACTCGCCGCCTCCAAC
GACCTGGTCGTGGAGGACCCGCGCTGGTGGCCGCACGTGCGGGCCGGTGACGACATCGCC
GGCAGCCAGCGGCTGACCTTCTGGGCCCCGGACGACGACGGCGGGTACGTGAACGTGTCC
GACGAGCTCGGCCTGGCCATCCCGGTGCCCACCCGCGGGATCGCGGTCGGCGACGCCGAC
GGCGACGGCCGGCTCGACATGGCCGTGGCCCGCCAGTGGGAGGAGCCCGTCTTCTACCAC
AACACCGCCCCGTCGCCGGGCGCCCACCTCAACCTGCGGCTGACGCACGACACCGAGGAC
GGCGACTCCGGATCGGGCGGCTCCGAGCCGGCCGAGGGCGCGCTGCCCGCGCCGGGCAGC
CCGGTCGTCGGCGCGCAGGTGAAGGTCACCACCCCGGACGGGCGCGCGCACCTGGCCCGG
GTGGACGGCGGCAGCGGCCACTCCGGCAAGCGCAGCGACGAGGTGCACATCGGCCTGGGC
GCGGGGGTCACCGGCCCCGTGCGGGTCGAGCTGTGCTGGCGGGACCGCTCGGGCACGGAG
CACCGCCAGGAGCTGCGGCTCGCCCCCGGCCGGCACTCGCTACGGCTCGGCGCCGGCGCC
GAAGTCGTCCGGCCCGACCAGCCCCAGGACACGCAGACCCCGGCCGAGGAGGAATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR011519 ASPIC/UnbV (Domain)
[581-654] 5.09999999999998e-20 PF07593
PF07593   UnbV_ASPIC
IPR013517 FG-GAP repeat (Repeat)
[201-235] 9.39999999999999e-06 PF01839
PF01839   FG-GAP
SignalP
[1-35] 0.583 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Madu_00450 Madu_00450   Madu_00470 Madu_00470
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