Madu_00530 : CDS information

Location

Organism	Actinomadura madurae
Strain	ATCC 39144
Entry name	Maduropeptin
Contig	AY271660
Start / Stop / Direction	81,719 / 80,148 / - [in whole cluster] 81,719 / 80,148 / - [in contig]
Location	complement(80148..81719) [in whole cluster] complement(80148..81719) [in contig]
Type	CDS
Length	1,572 bp (523 aa)

Annotation

Category	5.1 general function
Product	putative M28 family peptidase
Product (GenBank)	predicted peptidase
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in maduropeptin biosynthesis.
Note (GenBank)	mdp rf5
Reference	ACC B0BLQ5 PmId [17918933] Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. (J Am Chem Soc. , 2007) comment Maduropeptin生合成遺伝子クラスターの報告 orf5: Predicted peptidase Maduropeptin生合成遺伝子クラスターは、遺伝子産物の推定機能よりmdpA1からmdpE2までとしている。このORFについて機能解析はしていない。
Related Reference	ACC Q5QHG6 PmId [15632434] ( , ) comment Trichophyton rubrum_LAP1 Aminopeptidase Y ruLap1, ruLap2, ruDppIV, ruDppVの解析報告。 recombinant酵素を用い、ruLap1がpolypeptide chainのKR siteで切断を行う事を明らかにした。また、ruLap1がLC-AMC(7-amido-4-methylcoumarin)特異的であることを示した。

Sequence

>putative M28 family peptidase [predicted peptidase]
MALPAATVFLLALSAVPPAGAAPAAPVALVGPGAAPDRPDLAELVTIEDVRRHLEAFQQI
GDYNGGNRASGTPGYEISVKYVVGRLRKAGFSPTVQKFTYPYWTERSVAVMSRAAGEGKG
KGKTKDKDKDKAYVAGLDFATMMYSGSGDVTARVIPVDVPAKGAEGTSGCEKADFKGFAK
GSIALMQRGKCTFETKAARARSAGAAAAIIYNKPGEKGPIDGTLDKPAKLPVVGASREVG
AELVKAAAKGGLTMRVKTDTEHGERSSSNVIVDTKQGRADNVVVVGAHLDSVQDGPGLND
NATGSAALLAVAEEIGALGEDGLRNRVRFAWWGAEEEGLHGSTHYVEKMDPSDRRNIAVN
LNFDMLGSPNGIRAVYDGDHSLGQGTTPPAGSAAIEKMFREYYRARDLPTAEAVFNGRSD
YGPFIDHDIPAGGLATGADGVKTAAEAKAYGGRAGKTYDPCYHAKCDRMKNVNMKLLDTN
VDGVAHVTQLLAASTVAVNGDARLLPAAVDAAGPLWSGGHLIR

>putative M28 family peptidase [predicted peptidase]
GTGGCGCTGCCCGCCGCGACCGTGTTCCTCCTGGCGCTGTCCGCCGTGCCGCCGGCCGGT
GCCGCTCCCGCCGCTCCGGTCGCGCTCGTCGGTCCGGGGGCGGCGCCCGACCGTCCCGAC
CTCGCCGAGCTGGTGACGATCGAGGACGTGCGGCGGCATCTGGAGGCGTTCCAGCAGATC
GGCGACTACAACGGCGGCAACCGCGCGTCCGGCACGCCCGGGTACGAGATCTCGGTGAAG
TACGTCGTCGGGCGGCTGCGCAAGGCGGGGTTCTCGCCGACCGTGCAGAAGTTCACCTAC
CCGTACTGGACGGAGCGTTCGGTCGCGGTGATGTCGCGAGCGGCAGGCGAGGGCAAGGGC
AAGGGCAAAACCAAGGACAAGGACAAGGACAAGGCGTACGTGGCGGGCCTGGACTTCGCC
ACGATGATGTACTCCGGTTCCGGGGACGTCACCGCCCGCGTGATCCCCGTGGACGTCCCG
GCCAAGGGCGCGGAGGGCACCAGCGGGTGCGAGAAGGCGGACTTCAAAGGCTTCGCCAAG
GGCTCGATCGCGCTGATGCAGCGCGGGAAGTGCACGTTCGAGACGAAGGCGGCGCGCGCG
CGGTCCGCCGGTGCGGCTGCGGCGATCATCTACAACAAGCCCGGTGAGAAGGGCCCGATC
GACGGGACGCTCGACAAGCCGGCGAAGCTGCCGGTGGTGGGCGCGAGCCGCGAGGTCGGC
GCCGAGCTGGTGAAGGCGGCGGCCAAGGGCGGCCTGACGATGCGGGTGAAGACCGACACC
GAGCACGGTGAGCGGTCGTCCAGCAACGTCATCGTCGACACGAAGCAGGGACGTGCCGAC
AACGTGGTGGTCGTGGGGGCGCACCTCGACAGCGTGCAGGACGGGCCGGGCCTCAACGAC
AACGCGACCGGGTCGGCCGCGCTGCTCGCGGTCGCGGAGGAGATCGGCGCGCTCGGCGAG
GACGGGCTGCGGAACCGGGTGCGGTTCGCCTGGTGGGGCGCCGAGGAGGAGGGGCTGCAC
GGTTCGACGCACTACGTCGAGAAGATGGACCCGTCCGACCGGCGGAACATCGCCGTGAAC
CTCAACTTCGACATGCTCGGCTCGCCGAACGGGATCCGCGCCGTGTACGACGGCGACCAC
TCGCTCGGCCAGGGCACCACCCCGCCGGCCGGTTCGGCGGCGATCGAGAAGATGTTCCGC
GAGTACTACAGGGCGCGCGACCTGCCGACCGCCGAGGCCGTCTTCAACGGGCGCTCGGAC
TACGGCCCGTTCATCGACCACGACATCCCGGCCGGGGGCCTCGCCACCGGTGCGGACGGC
GTCAAGACGGCGGCCGAGGCGAAGGCGTACGGGGGGCGCGCGGGCAAGACCTACGACCCG
TGCTACCACGCGAAGTGCGACCGGATGAAGAATGTGAACATGAAGCTGCTGGACACGAAC
GTCGACGGCGTCGCGCACGTCACGCAGTTGCTCGCGGCCAGCACGGTCGCGGTGAACGGC
GACGCCCGGCTGCTGCCGGCGGCGGTCGACGCGGCGGGCCCGCTGTGGAGCGGCGGTCAC
CTGATCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003137 Protease-associated domain, PA (Domain) PF02225 PA IPR007484 Peptidase M28 (Domain) PF04389 Peptidase_M28
SignalP	Eukaryota Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Madu_00520 last last