C1027_00060 C1027_00060   C1027_00080 C1027_00080

C1027_00070 : CDS information

close this sectionLocation

Organism
StrainC-1027
Entry nameC-1027
Contig
Start / Stop / Direction9,927 / 7,573 / - [in whole cluster]
9,927 / 7,573 / - [in contig]
Locationcomplement(7573..9927) [in whole cluster]
complement(7573..9927) [in contig]
TypeCDS
Length2,355 bp (784 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
Productputative UvrA-like protein
Product (GenBank)UvrA-like drug resistance pump
GenesgcB2
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF(-1)
Reference
ACC
PmId
[12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002)
comment
C-1027の生合成遺伝子クラスターの報告

Fig.2にてcluster内に表示されているのみ
Related Reference
ACC
Q54827
NITE
Adria_00370
PmId
[8655504] The Streptomyces peucetius drrC gene encodes a UvrA-like protein involved in daunorubicin resistance and production. (J Bacteriol. , 1996)
[9835035] The DrrC protein of Streptomyces peucetius, a UvrA-like protein, is a DNA-binding protein whose gene is induced by daunorubicin. (FEMS Microbiol Lett. , 1998)
comment
Streptomyces peucetius_drrC
Daunorubicin resistance protein

[PMID: 8655504]
DrrCはDNAの除去修復に関連するE.coli_UvrAに強いAA配列類似あり。
S.lividansへのdrrC導入はdaunorubicin (DNR)抵抗性を与える。
DNR and doxorubicin (DXR) antiporterによって抵抗性をもたらすDrrABのシステムと異なり、たぶんゲノムDNAへのDNR and DXRの結合を抑制または不安定化をしうる。

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同じ著者らの続報
[PMID: 9835035]
E.coliで発現して得たDrrCは、ATPとDNRの存在下でのみ、DNR産生遺伝子のpromoter領域を含むDNA segmentを結合。
S.peucetiusにおけるDrrCの出現はDNR産生の開始と同時に起こり、drrC geneはDNRによって誘導される。
DnrN and DnrI regulatory proteinsはdrrC発現のために必要。
DrrAは、DrrCと同じ様式で調節された。

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参考[PMID: 20158521]
この論文での実験にdrrCは絡んでいないが、総合的なFigあり。

細胞内DNR濃度が上昇したときに、DNR biosynthetic genes and resistance genesのactivatorであるDnrIのpromoter領域へfeedback阻害のために結合したDNR（本来はDnrNが結合）を、再活性化のために離すことに関連しているのではないかと言っている。

close this sectionSequence

selected fasta
>putative UvrA-like protein [UvrA-like drug resistance pump]
MYGSQEENSVSVTDPTAGAGKTSGPGDGIRIAGARIHNLKDVSLTIPRNQITVFTGVSGS
GKSSIVFDTVAVEAQRQLNSTFSWYIRNQLPKYERPQAEAIENLTTPVIVDQKPVGGNAR
STVGTMTDVQPMIRALFARFGTTKDDGPSSLGVSAFSFNDPQGMCPDCDGLGQSIALDLD
KMLDRSKSLDDGAVLFPEYKVGSPDWQIWAKSGRLDPAKPIAEYSAEELDTLLRGTGGKV
TLKTKSTEFQTNYEGLADRFERLNLKRDLSALSDRKREVIERFVTDGVCPSCRGARLNAA
ALDVRIDGKNIADYSSMEVRDLTEVLAGVTEPAAAPLAKAARTALERIVSIGLGYLTLDR
PTADLSGGEGQRLKMVRHLGSGLAGLTYIFDEPSIGLHPRDVGRLNDLLRALRDKGNTVL
VVEHDPDVIEIADHIVDVGPGAGVHGGEIVFEGSFAKLRKAKTRTGEALRRGARVKEEVR
PPTGELTVENADLHNLKQVSVAVPTGVLTAVTGVAGSGKSSLISGAFMEAHPDAVFVDQS
AIAASSRSTPVSYLGLMDPLRKLFAKETGANASLFSFNSKGSCEECQGRGVIITELAFMD
PVTTHCGVCEGRRFKPEVLEHQLRGKSIADVLELPADEAVEFFTEKALLPKLRALVDVGL
GYLSLGQPLSSLSGGELQRIKLADQLHRTGTVYVLDEPTTGLHMSDVDTLLKLLDGLVEA
GNTVVVIEHNLDVVQQADWIIDLGPDGGREGGEIVFTGTPKDLLAADSSLTGEYLRRHLK
AQAG
selected fasta
>putative UvrA-like protein [UvrA-like drug resistance pump]
ATGTACGGAAGCCAGGAGGAAAACTCTGTGAGCGTCACCGACCCCACCGCCGGCGCGGGA
AAAACATCCGGCCCGGGAGACGGCATCCGCATCGCGGGCGCGCGGATCCACAACCTCAAA
GATGTCTCCCTGACGATTCCCCGCAACCAGATCACGGTCTTCACGGGGGTCTCGGGTTCG
GGCAAGTCGTCGATCGTCTTCGACACGGTGGCCGTGGAGGCCCAGCGACAGCTGAACTCC
ACCTTCAGCTGGTACATCCGCAACCAGCTTCCGAAGTACGAACGTCCTCAGGCCGAGGCG
ATCGAGAACCTCACCACCCCGGTGATCGTCGACCAGAAGCCCGTGGGGGGCAACGCCCGG
TCCACGGTGGGGACCATGACGGACGTACAGCCGATGATCCGGGCCCTGTTCGCACGGTTC
GGCACGACGAAGGACGACGGGCCGTCCTCGCTCGGCGTCTCCGCGTTCTCGTTCAACGAC
CCCCAGGGCATGTGCCCGGACTGCGACGGTCTCGGCCAGAGCATCGCCCTCGACCTGGAC
AAGATGCTGGACCGCTCGAAGTCCCTGGACGACGGGGCGGTCCTTTTCCCCGAGTACAAG
GTGGGTAGCCCGGACTGGCAGATCTGGGCGAAGTCCGGCCGTCTGGATCCGGCCAAGCCG
ATCGCCGAGTACAGCGCCGAGGAGCTGGACACGCTGCTGCGCGGCACCGGCGGGAAGGTG
ACGCTGAAGACCAAGAGCACCGAGTTCCAGACGAACTACGAGGGGTTGGCCGACCGGTTC
GAGCGGCTCAACCTCAAGCGCGACCTCAGCGCGCTGAGCGACCGCAAGCGCGAGGTCATC
GAACGTTTCGTCACCGACGGAGTCTGTCCCTCCTGCCGGGGCGCGCGGCTCAACGCCGCC
GCGCTCGACGTCCGGATCGACGGCAAGAACATCGCCGACTACTCGAGCATGGAGGTCCGC
GACCTCACGGAGGTGCTCGCCGGCGTCACGGAGCCGGCCGCTGCCCCTCTCGCGAAAGCC
GCGCGCACGGCCCTGGAGCGGATCGTGTCCATCGGGCTCGGCTACCTGACCCTGGACAGG
CCCACGGCCGACCTCTCGGGGGGCGAGGGGCAGCGGCTGAAGATGGTGCGCCACCTCGGC
AGTGGTCTGGCCGGGCTCACGTACATCTTCGACGAGCCCAGCATCGGCCTGCACCCGCGC
GACGTGGGGCGGCTCAACGACCTGCTGCGCGCGCTGCGCGACAAGGGGAACACCGTGCTG
GTCGTCGAGCACGACCCCGATGTGATCGAGATCGCCGACCACATCGTCGATGTCGGTCCC
GGGGCGGGAGTGCACGGGGGCGAGATCGTGTTCGAGGGGTCCTTCGCGAAGCTGCGGAAG
GCGAAGACCCGGACCGGGGAGGCTCTGCGGCGGGGGGCCCGCGTGAAGGAGGAGGTCCGC
CCGCCCACGGGGGAGCTGACCGTGGAGAACGCGGATCTCCACAACCTGAAGCAGGTCTCC
GTGGCCGTGCCCACCGGTGTGCTGACCGCGGTGACCGGGGTGGCGGGTTCCGGCAAGAGC
AGCCTGATCTCCGGGGCATTCATGGAGGCGCACCCCGACGCGGTCTTTGTCGACCAGTCC
GCGATCGCCGCCTCGTCGCGCTCCACACCGGTCTCCTACCTCGGCCTGATGGACCCGCTG
CGCAAGCTGTTCGCGAAGGAGACCGGCGCGAACGCCTCGCTGTTCAGCTTCAACTCCAAG
GGGTCGTGCGAGGAGTGCCAGGGCCGCGGGGTCATCATCACCGAGCTGGCCTTCATGGAT
CCGGTGACCACGCACTGCGGTGTCTGTGAGGGGCGCCGCTTCAAGCCCGAGGTCCTGGAG
CACCAGCTTCGCGGGAAGTCGATCGCGGACGTCCTGGAGCTGCCCGCCGACGAGGCCGTC
GAGTTCTTCACCGAGAAGGCCCTGCTGCCGAAACTGCGCGCTCTCGTCGACGTCGGTCTT
GGGTACCTCAGCCTGGGCCAGCCGCTTTCCAGCCTCTCCGGCGGTGAACTGCAGCGGATC
AAACTTGCCGACCAGCTGCACCGCACGGGCACGGTCTATGTCCTGGACGAGCCCACCACC
GGGCTGCACATGTCGGACGTTGACACCCTGCTGAAGCTCCTGGACGGGCTCGTCGAGGCC
GGGAACACCGTCGTGGTCATCGAGCACAACCTCGATGTGGTGCAGCAGGCCGACTGGATC
ATCGACCTGGGTCCCGACGGGGGACGGGAGGGCGGCGAGATCGTCTTCACCGGCACACCG
AAGGACCTGCTCGCCGCGGATTCCTCCCTGACCGGTGAGTATCTGCGCCGCCACCTGAAG
GCGCAGGCGGGTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003439 ABC transporter-like (Domain)
[10-465] PS50893 [473-770] PS50893
PS50893   ABC_TRANSPORTER_2
[652-700] 6.50000000000001e-09 PF00005
PF00005   ABC_tran
IPR017871 ABC transporter, conserved site (Conserved_site)
[672-686] PS00211
PS00211   ABC_TRANSPORTER_1
SignalP No significant hit
TMHMM No significant hit
C1027_00060 C1027_00060   C1027_00080 C1027_00080
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