C1027_00090 : CDS information

Location

Organism	Streptomyces globisporus
Strain	C-1027
Entry name	C-1027
Contig	AY048670
Start / Stop / Direction	12,835 / 11,351 / - [in whole cluster] 12,835 / 11,351 / - [in contig]
Location	complement(11351..12835) [in whole cluster] complement(11351..12835) [in contig]
Type	CDS
Length	1,485 bp (494 aa)

Annotation

Category	3.4 other modification
Product	FADH2-dependent (S)-beta-tyrosyl-PCP halogenase
Product (GenBank)	alkylhalidase
Gene	sgcC3
Gene (GenBank)
EC number
Keyword	two-component system (S)-3-chloro-4,5-dihydroxy-beta-phenylalanine
Note
Note (GenBank)	ORF11
Reference	ACC Q8GMI1 PmId [12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002) [16104723] Biosynthesis of the beta-amino acid moiety of the enediyne antitumor antibiotic C-1027 featuring beta-amino acyl-S-carrier protein intermediates. (J Am Chem Soc. , 2005) [17887753] Regiospecific chlorination of (S)-beta-tyrosyl-S-carrier protein catalyzed by SgcC3 in the biosynthesis of the enediyne antitumor antibiotic C-1027. (J Am Chem Soc. , 2007) comment [PMID:12183628] C-1027の生合成遺伝子クラスターの報告 sgcC to sgcC6, encoding beta-amino biosynthesis. halogenase(SgcC3)としている。機能解析はしていない。 [PMID:16104723] C-1027のbeta-amino acid moiety生合成の報告 SgcC2のFT-MSの結果と、SgcC1のアミノ酸依存性のATP-[32P]PPi exchange dataは、SgcC1が(S)-beta-tyrosine特異的なadenylation enzymeで、SgcCとSgcC3はbeta-aminoacyl-S-SgcC2を基質として利用すると示した。また、sgcC3,sgcCの各欠損株の産物より、SgcC3はSgcCよりも前に働くと推定している。 [PMID:17887753] SgcC3の機能解析 SgcC3 は、L-alpha-tyrosineから合成される(S)-3-chloro-4,5-dihydroxy-β-phenylalanine moietyの生合成において３番目のenzymatic transformationを触媒する事を明らかにした。 SgcC3はflavin reductase component(sgcE6)からreduced flavin(FADH2)を得てtwo-component systemとして機能し、SgcC2につながれた基質のchlorinationとbrominationを行うことを示した。

Sequence

>FADH2-dependent (S)-beta-tyrosyl-PCP halogenase [alkylhalidase]
MDVSAQYDVIVVGGGPAGSTVSTLVRKRGHRVLQLEKETFPRYQIGESLLPSTVHGIAHL
LGVSDELKKAAFTIKHGGTFKWGANPEPWTFDFAVSRRMPGATGYAYQVERMKFDQILLD
NARRHGVEVRENSDVLDVLKAEDGRVRGVRYRDSEGREHEVGSRFVVDASGNTGGLYKKS
GAKREYSPFFRNLALFGYFNGGKRLPKPNSGNIFTCTFEHGWFWYIPLSPELTSVGAVVN
RDSASLVQGDPEKAMESFIAACPLIAEKLSEATRVTEGPYGELRVRKDWSYSNTKFWAPG
MALVGDAACFVDPVFSSGVHLATYSGLLAARSLNSCLDGSVDETSAFEEFEARYRLEYGR
FYEFLVGFYDMHHSEDSYFWQARKISNTADSDLQSFVELIGGVSSDEFADTARARQRFSE
ASDEMAGMTHTAAGDADGPHGAVPGKVVGRLMEQSAALQARALLGDEAGAERPVRQGGLA
ASTDGLGWVPGARS

>FADH2-dependent (S)-beta-tyrosyl-PCP halogenase [alkylhalidase]
GTGGACGTGTCAGCGCAGTACGACGTCATCGTGGTCGGCGGAGGTCCGGCGGGCTCGACC
GTCTCCACGCTGGTCCGCAAGCGGGGCCATCGCGTACTCCAGCTGGAGAAGGAGACCTTC
CCGCGCTACCAGATCGGTGAGTCGCTCCTCCCCTCCACCGTCCACGGCATCGCACACCTC
CTCGGTGTCTCCGACGAGCTGAAGAAGGCAGCCTTCACGATCAAGCACGGCGGGACGTTC
AAGTGGGGTGCCAACCCCGAACCGTGGACGTTCGACTTCGCCGTCTCGCGCCGGATGCCG
GGGGCGACGGGGTACGCGTACCAGGTCGAGCGCATGAAGTTCGACCAGATCCTGCTCGAC
AACGCTCGCAGGCACGGTGTCGAGGTGCGGGAGAACAGCGATGTCCTCGACGTGCTCAAG
GCCGAGGACGGCCGGGTGCGGGGCGTGCGCTACCGGGACTCCGAGGGGCGCGAGCACGAG
GTGGGCTCCCGTTTCGTCGTGGACGCCTCCGGCAACACCGGTGGGCTGTACAAGAAGTCG
GGCGCCAAGCGCGAGTACTCGCCCTTCTTCCGCAACCTGGCCCTGTTCGGCTACTTCAAC
GGCGGCAAGCGGCTTCCGAAGCCGAACTCCGGGAACATCTTCACCTGCACCTTCGAGCAC
GGGTGGTTCTGGTACATACCCCTGTCTCCCGAGCTGACGAGTGTCGGCGCAGTGGTGAAC
CGGGACTCGGCGAGCCTGGTGCAGGGCGACCCCGAGAAGGCGATGGAGTCGTTCATCGCG
GCCTGCCCGCTGATCGCGGAGAAGCTCTCCGAGGCCACCCGGGTGACCGAGGGCCCCTAC
GGCGAGCTCCGGGTCCGCAAGGACTGGTCGTACAGCAACACGAAGTTCTGGGCGCCGGGC
ATGGCCCTGGTCGGGGATGCCGCGTGCTTCGTCGACCCGGTGTTCTCCTCCGGGGTGCAC
CTCGCCACCTACAGCGGACTGCTGGCAGCGCGCTCGCTCAACTCCTGCCTGGACGGGTCG
GTCGACGAGACCAGCGCGTTCGAGGAGTTCGAGGCCCGCTACCGGCTGGAGTACGGCCGC
TTCTACGAGTTCCTGGTCGGCTTCTACGACATGCACCATTCGGAGGACTCCTATTTCTGG
CAGGCCCGGAAGATCTCCAACACCGCGGACTCCGACCTGCAGTCCTTCGTCGAACTGATC
GGCGGGGTCTCCTCCGACGAGTTCGCCGACACGGCCCGGGCCAGGCAGCGGTTCTCCGAG
GCGTCCGACGAGATGGCCGGCATGACGCACACCGCTGCGGGCGACGCCGACGGGCCGCAC
GGCGCCGTGCCGGGAAAGGTGGTGGGCCGCCTCATGGAGCAGAGCGCCGCACTGCAGGCA
CGGGCTCTTCTGGGGGATGAGGCGGGGGCCGAGCGACCGGTGAGGCAGGGGGGTCTGGCC
GCGTCGACGGACGGGCTGGGCTGGGTTCCCGGTGCGCGGTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003042 Aromatic-ring hydroxylase-like (Domain) PR00420 RNGMNOXGNASE IPR006905 Tryptophan halogenase (Family) PF04820 Trp_halogenase
SignalP	No significant hit
TMHMM	No significant hit

C1027_00080 C1027_00100