C1027_00090 C1027_00090   C1027_00110 C1027_00110

C1027_00100 : CDS information

close this sectionLocation

Organism
StrainC-1027
Entry nameC-1027
Contig
Start / Stop / Direction13,012 / 14,079 / + [in whole cluster]
13,012 / 14,079 / + [in contig]
Location13012..14079 [in whole cluster]
13012..14079 [in contig]
TypeCDS
Length1,068 bp (355 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productglucose-1-phosphate thymidylyltransferase
dTDP-glucose synthase
Product (GenBank)dNTP-glucose synthase
GenesgcA1
Gene (GenBank)
EC number2.7.7.24
Keyword
  • deoxy aminosugar
Note
Note (GenBank)
  • ORF10
Reference
ACC
PmId
[12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002)
[14987060] Biochemical characterization of the SgcA1 alpha-D-glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne antitumor antibiotic C-1027 biosynthetic pathway and overexpression of sgcA1 in Streptomyces globisporus to improve C-1027 production. (J Nat Prod. , 2004)
comment
[PMID:12183628]
C-1027の生合成遺伝子クラスターの報告

sgcA to sgcA6, encoding deoxyaminosugar biosynthesis.
機能解析はしていない。
thymine diphosphate(TDP)-glucose synthetase(SgcA1)としている。


[PMID:14987060]
SgcA1の機能解析報告　

大腸菌にてsgcA1を過剰発現させ、精製し、生化学的(分子量,pH依存性,金属イオン要求性,基質特異性)および酵素学的解析を行った。
精製酵素はalpha-D-glucopyranosyl-1-phosphate thymidylyltransferaseとして特徴付けられ、sgcA1の過剰発現株はC-1027の生産量が改善した。

close this sectionSequence

selected fasta
>glucose-1-phosphate thymidylyltransferase [dNTP-glucose synthase]
MKALVLSGGSGTRLRPISYAMPKQLVPIAGKPVLEYVLDNIRNLDIKEVAIVVGDWAQEI
IEAMGDGSRFGLRLTYIRQEQPLGIAHCVKLARDFLDEDDFVLYLGDIMLDGDLSAQAGH
FLHTRPAARIVVRQVPDPRAFGVIELDGEGRVLRLVEKPREPRSDLAAVGVYFFTADVHR
AVDAISPSRRGELEITDAIQWLLEQGLPVEAGRYTDYWKDTGRVEDVVECNRRMLGRLAL
QVSGEVDPESELVGAVVVEEGARVTRSRVVGPAVIGAGTVVEDSQIGPYASIGRRCTVRA
SRLSDSIVLDDASILAVSGLHGSLIGRGARIAPGARGEARHRLVVGDHVQIEIAA
selected fasta
>glucose-1-phosphate thymidylyltransferase [dNTP-glucose synthase]
ATGAAGGCACTTGTACTGTCGGGTGGTTCGGGGACCCGCCTGCGCCCGATCAGTTACGCC
ATGCCGAAGCAGCTCGTTCCGATCGCCGGGAAGCCAGTCCTTGAATATGTTCTGGATAAT
ATCCGGAACCTCGATATCAAAGAGGTCGCCATTGTCGTCGGTGACTGGGCTCAGGAAATT
ATTGAGGCAATGGGTGACGGCAGCCGTTTCGGTCTGCGCCTCACCTACATACGCCAGGAG
CAACCTCTGGGCATCGCGCACTGCGTGAAACTGGCCCGAGACTTCCTCGACGAGGACGAC
TTCGTCCTCTACCTAGGCGACATCATGCTGGACGGAGACCTGTCCGCGCAGGCGGGGCAC
TTCCTCCACACCCGCCCCGCCGCGCGGATCGTCGTGCGCCAGGTGCCCGACCCCCGGGCC
TTCGGGGTGATCGAGCTGGACGGCGAAGGGCGTGTGCTGCGCCTGGTCGAGAAACCCCGT
GAACCGCGCAGCGACCTCGCGGCGGTCGGCGTGTACTTCTTCACCGCGGACGTGCACCGC
GCCGTCGACGCGATTAGCCCGAGCCGACGGGGCGAGCTGGAAATCACCGACGCCATCCAG
TGGCTGCTGGAGCAGGGCCTGCCGGTCGAGGCCGGCCGCTACACGGACTACTGGAAGGAC
ACCGGCCGGGTCGAGGACGTCGTGGAGTGCAACCGGCGGATGCTCGGCCGTCTGGCGCTC
CAGGTGTCGGGCGAGGTGGACCCGGAGAGCGAACTGGTGGGTGCGGTGGTCGTCGAGGAG
GGCGCCCGGGTGACGCGTTCGCGGGTCGTGGGACCAGCGGTGATCGGCGCGGGCACGGTC
GTCGAGGACAGCCAGATCGGACCGTACGCCTCCATCGGCCGGCGCTGCACCGTGCGGGCG
TCCCGGCTCTCCGACTCCATCGTCCTTGACGACGCCTCGATCCTCGCGGTGAGCGGACTG
CACGGCTCGCTGATCGGAAGGGGCGCGCGGATCGCGCCCGGGGCCCGGGGCGAGGCCCGG
CACCGGCTGGTCGTCGGCGACCACGTGCAGATCGAGATCGCGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005835 Nucleotidyl transferase (Domain)
[2-234] 2.89999999999999e-53 PF00483
PF00483   NTP_transferase
IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family)
[2-352] 7.40000000000007e-119 TIGR01208
TIGR01208   RmlA_long
SignalP No significant hit
TMHMM No significant hit
C1027_00090 C1027_00090   C1027_00110 C1027_00110
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