C1027_00260 : CDS information

Location

Organism	Streptomyces globisporus
Strain	C-1027
Entry name	C-1027
Contig	AY048670
Start / Stop / Direction	31,275 / 32,585 / + [in whole cluster] 31,275 / 32,585 / + [in contig]
Location	31275..32585 [in whole cluster] 31275..32585 [in contig]
Type	CDS
Length	1,311 bp (436 aa)

Annotation

Category	3.4 other modification
Product	putative ligase
Product (GenBank)	coenzyme F390 synthase-like protein
Gene	sgcH
Gene (GenBank)
EC number
Keyword	benzoxazolinate
Note
Note (GenBank)	ORF3
Reference	ACC Q8GMG7 PmId [12183628] Biosynthesis of the enediyne antitumor antibiotic C-1027. (Science. , 2002) [18182490] Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism. (Proc Natl Acad Sci U S A. , 2008) comment [PMID:12183628] C-1027の生合成遺伝子クラスターの報告本文中特にふれていない。 [PMID:18182490] この論文内でbenzoxazolinate生合成推定経路が示されており、benzoxazolinate moiety生合成における最終段階のthio-esterificationに関与すると推定している。機能解析はされていない。
Related Reference	ACC O33469 PmId [8969218] Molecular cloning and expression in different microbes of the DNA encoding Pseudomonas putida U phenylacetyl-CoA ligase. Use of this gene to improve the rate of benzylpenicillin biosynthesis in Penicillium chrysogenum. (J Biol Chem. , 1996) comment Pseudomonas putida_paaK(paaF, pcl) Phenylacetate-coenzyme A ligase (phenylacetyl-CoA ligase) 31%id, 1e-47 phenylacetyl-CoA ligaseをコードするpclをcloningし大腸菌にて発現、 phenylacetyl-CoA ligase activityをみている。
Family/Domain	FD IPR000873 comment [IPR000873] AMP-dependent synthetase/ligase (Domain)

Sequence

>putative ligase [coenzyme F390 synthase-like protein]
MEYWNSTAETMPRQELEQWKWRRLQAAMDHARRLSPFWRERLPENITSMADYAARVPLLR
KADLLAAEAASPPYGTWPSLDPALGVRHHQTSGTSGNPPIRTFDTERDWAWCVDTFCTAL
HSMGVRPHHKGLVAFGYGLFAGFWGMHYGLERMGATVIPAGGLDSRSRVRLLVDYQIEVL
GLTPSYAMRLIETAREMGIDLAREANVQIILAGAEPRSAFTTRTIEEAFGARVFNAAGTT
EFGGVFMFECTARREACHIIEPSCIEEVLDPVTEQPVGYGEEGVRVTTGLNREGMQLFRH
WTEDVVVKRPHTECGCGRTWDFYDGGILRRVDDMRKIRGVSITPVMIEDVLRGFDEVNEF
HSSIRTVRGLDTIHVKVEAGDISGEAAESLCGRITEEFKREIGIRPQVELTPAGSLPRSK
WKAARLHDERELAPQA

>putative ligase [coenzyme F390 synthase-like protein]
GTGGAGTACTGGAACAGCACGGCGGAGACCATGCCCCGCCAGGAACTCGAACAGTGGAAG
TGGCGCAGGCTCCAGGCCGCCATGGACCACGCCAGAAGGCTTTCGCCCTTCTGGCGGGAA
CGACTCCCCGAGAACATCACCTCCATGGCGGACTACGCGGCGCGGGTGCCTCTCCTGCGC
AAGGCCGACCTCCTCGCCGCGGAAGCCGCGTCTCCCCCTTACGGCACCTGGCCCTCGCTG
GATCCGGCGCTCGGAGTGCGCCATCACCAGACCAGCGGCACCAGCGGTAACCCCCCCATC
CGGACGTTCGACACCGAACGCGACTGGGCCTGGTGCGTGGACACGTTCTGCACGGCGCTC
CACAGCATGGGCGTGCGCCCGCACCACAAGGGTCTGGTGGCGTTCGGCTACGGGCTGTTC
GCCGGTTTCTGGGGCATGCACTACGGCCTCGAGCGCATGGGCGCCACGGTCATCCCGGCC
GGCGGCCTCGACTCCCGCTCCCGGGTACGGCTGCTGGTCGACTACCAGATCGAGGTGCTC
GGCCTCACACCGAGCTATGCGATGCGGCTGATCGAGACGGCCCGCGAGATGGGCATCGAC
CTCGCCCGCGAGGCTAACGTCCAGATCATCCTGGCCGGGGCGGAGCCGCGCTCCGCGTTC
ACCACCCGCACCATCGAGGAGGCCTTCGGCGCCCGGGTCTTCAACGCCGCGGGCACCACT
GAGTTCGGGGGGGTGTTCATGTTCGAGTGCACCGCCCGGCGCGAGGCCTGCCACATCATC
GAACCCTCGTGCATCGAGGAGGTGCTCGACCCGGTGACGGAACAGCCCGTCGGCTACGGC
GAGGAGGGCGTCCGAGTCACCACCGGGCTGAACCGTGAGGGGATGCAGCTCTTCCGGCAC
TGGACCGAGGACGTCGTGGTCAAGCGGCCCCACACCGAGTGCGGCTGCGGCCGGACGTGG
GACTTCTACGACGGCGGCATCCTTCGGCGCGTGGACGACATGCGCAAGATACGCGGGGTC
TCGATCACCCCGGTGATGATCGAGGATGTGCTGCGCGGCTTCGACGAGGTGAACGAGTTC
CACTCGTCCATCCGGACCGTCCGCGGACTCGATACGATCCACGTCAAGGTCGAGGCGGGA
GACATCTCGGGTGAGGCGGCCGAGAGCCTGTGCGGCCGCATCACCGAGGAGTTCAAGCGT
GAGATAGGCATACGGCCCCAGGTGGAGCTGACCCCCGCGGGCAGCCTCCCCCGATCGAAG
TGGAAGGCGGCACGACTTCATGACGAGCGCGAACTCGCCCCTCAGGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding
SignalP	No significant hit
TMHMM	No significant hit

C1027_00250 C1027_00270