Calm_00140 Calm_00140   Calm_00160 Calm_00160

Calm_00150 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction14,103 / 15,017 / + [in whole cluster]
14,103 / 15,017 / + [in contig]
Location14103..15017 [in whole cluster]
14103..15017 [in contig]
TypeCDS
Length915 bp (304 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative homocysteine S-methyltransferase
Product (GenBank)CalE2
GenecalE2
mmuM
Gene (GenBank)calE2
EC number2.1.1.10
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
comment
calicheamicin生合成遺伝子クラスターの報告

polyketide construction or modificationに関与するORFとしている。機能解析はされていない。
Related Reference
ACC
Q47690
PmId
[9882684] ( , )
comment
Escherichia coli
Homocysteine S-methyltransferase_mmuM(yagD)
29%id, 4e-19

YagD (=MmuM)のmutantを作製し機能解析している。
YagDは、基質としてselenohomocysteineをを受け入れるS-methylmethionine: homocysteine methyltransferase であると示した。

close this sectionSequence

selected fasta
>putative homocysteine S-methyltransferase [CalE2]
MDVPGAPTAGPLVLDGGLGTELQRHGRSVSAPWWTARCLLDAGGRRLVSRVHAAYVAAGA
DVLTADTFRTTLRTAYRAGTDEATAAGLVRTAVTLARRAADSTGAGRRVLVAASVAPVED
CYRPDLVPGAAVLRREHGWLADQLARAGVDLALVETMNTVREAVVATRAVREHGLPAWVS
FVCTGDARLLSGEDLAAAADAVRAAGAAAVLVNCTDPAGTERALRRLRAAGPGLLGAYPN
VEDRSGVPPATPVDRYLPPGLTPDAFADLWAAWRPVGLRIVGGCCGTTPAHIAALRSRLA
PRPG
selected fasta
>putative homocysteine S-methyltransferase [CalE2]
GTGGACGTACCGGGGGCGCCGACGGCCGGGCCACTGGTCCTCGACGGCGGGCTGGGCACC
GAGCTGCAACGGCACGGCCGGTCCGTGTCCGCCCCGTGGTGGACGGCACGCTGCCTGCTG
GACGCCGGGGGCCGGCGCCTCGTCTCCCGCGTCCACGCCGCGTACGTGGCGGCGGGAGCG
GACGTGCTCACCGCGGACACGTTCCGCACCACCCTGCGGACGGCGTACCGGGCCGGAACG
GACGAGGCCACGGCCGCCGGTCTGGTCCGTACCGCCGTGACGCTGGCCCGCCGCGCGGCC
GATTCGACGGGGGCCGGCCGGCGGGTGCTCGTCGCCGCCTCCGTCGCCCCGGTCGAGGAC
TGCTACCGGCCGGACCTGGTGCCCGGCGCCGCGGTGCTGCGGCGGGAGCACGGCTGGCTG
GCCGACCAGCTTGCCCGCGCCGGCGTCGACCTGGCGCTGGTCGAGACCATGAACACCGTC
CGGGAGGCGGTCGTCGCCACCCGGGCGGTACGCGAACACGGCCTGCCGGCCTGGGTGAGC
TTCGTCTGCACCGGCGACGCCCGCCTGCTGTCCGGTGAGGACCTCGCCGCGGCGGCCGAC
GCGGTACGGGCGGCCGGCGCGGCTGCGGTCCTGGTCAACTGCACCGACCCGGCGGGCACC
GAACGCGCGCTGCGGCGGCTCCGCGCCGCCGGGCCGGGCCTGCTCGGCGCGTACCCGAAC
GTGGAGGACCGCAGCGGCGTGCCGCCGGCCACCCCGGTGGACCGCTATCTCCCGCCCGGC
CTGACGCCCGACGCCTTCGCCGACCTCTGGGCGGCCTGGCGCCCCGTCGGGCTGCGCATC
GTCGGCGGTTGCTGCGGCACCACCCCGGCGCACATCGCCGCGTTGCGGTCCCGCCTGGCG
CCGCGCCCGGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003726 Homocysteine S-methyltransferase (Domain)
[10-302] 1.20000000000001e-77 G3DSA:3.20.20.330
G3DSA:3.20.20.330   S_methyl_trans
[12-299] 1.5e-46 PF02574
PF02574   S-methyl_trans
[1-299] PS50970
PS50970   HCY
[9-302] 4.69998262513189e-74 SSF82282
SSF82282   S_methyl_trans
SignalP No significant hit
TMHMM No significant hit
Calm_00140 Calm_00140   Calm_00160 Calm_00160
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