Calm_00150 Calm_00150   Calm_00170 Calm_00170

Calm_00160 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction16,092 / 15,028 / - [in whole cluster]
16,092 / 15,028 / - [in contig]
Locationcomplement(15028..16092) [in whole cluster]
complement(15028..16092) [in contig]
TypeCDS
Length1,065 bp (354 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative adenosine deaminase
Product (GenBank)CalS5
Gene
Gene (GenBank)calS5
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
comment
calicheamicin生合成遺伝子クラスターの報告

このORFは、Fig2の生合成clusterにてexpected production of four unusual activated nucleotide sugars (S1 to S14)として示されている。本文中ふれていない。
Related Reference
ACC
P22333
PmId
[1998686] ( , )
comment
Escherichia coli
Adenosine deaminase_add
29%id, 1e-36

E.coliのadenosine deaminase欠損株での機能的相補テストにより、この活性を持つ遺伝子を単離。またそこからアミノ酸配列を推定して他種配列との比較を行っている。

close this sectionSequence

selected fasta
>putative adenosine deaminase [CalS5]
MTTAVPAGQDLTDVLGRLPKVELHVHLEGSVRPGTLLRIARRHGLDLGRLDEAAVVELFR
FRDFRHFTELYEQCCGALRDPADLELITEELADEAHRQNVRHLEVTFSPGTHRRGAGIPF
DEQLDAVARGAEQARRRTGVTMRFVVDHVRGGPPEECQQVAEWAVAGAGRGVVALGLGGF
EPRRPASLFGEAVRWAAARGVPFVPHAGEAVGPEGVWDCLPFRPPRIGHGIRSVEDPRLV
AALRDRAVVLEVCPTSNLRTGVVSEPGAHPLRRLWDAGVRLTLNTDDPSMFHTDLLAEHR
FAVEWHGFTVAELAAMTLIGVDAALLPATEREALRERVTTELAELGVTAAGLPA
selected fasta
>putative adenosine deaminase [CalS5]
GTGACGACCGCCGTACCAGCCGGGCAGGATCTCACCGACGTCCTTGGCCGCCTGCCCAAG
GTCGAGTTGCACGTGCACCTCGAAGGGTCGGTGCGGCCCGGCACCCTGCTCCGGATCGCC
CGCCGGCACGGCCTCGACCTTGGTCGGCTCGACGAGGCGGCGGTGGTGGAACTCTTCCGC
TTCCGCGACTTCCGCCACTTCACAGAACTGTACGAACAGTGCTGCGGGGCGCTGCGCGAC
CCGGCCGACCTCGAACTGATCACCGAGGAACTCGCCGACGAGGCGCACCGGCAGAACGTC
CGCCACCTGGAGGTGACGTTCAGCCCCGGCACGCACCGGCGGGGAGCGGGCATCCCGTTC
GACGAGCAGCTCGACGCCGTGGCCCGGGGCGCCGAGCAGGCCCGCCGCCGCACCGGCGTC
ACGATGCGCTTCGTCGTCGACCACGTCCGGGGCGGGCCGCCGGAGGAGTGCCAGCAGGTC
GCCGAGTGGGCGGTCGCCGGCGCCGGCCGGGGCGTGGTGGCGCTCGGTCTCGGCGGTTTC
GAGCCGCGGCGCCCGGCCTCGCTGTTCGGGGAGGCCGTCCGGTGGGCGGCGGCGCGGGGC
GTGCCGTTCGTGCCGCACGCGGGCGAGGCGGTCGGGCCCGAGGGCGTCTGGGACTGCCTG
CCGTTCCGCCCGCCGCGCATCGGGCACGGCATCCGCTCCGTCGAGGACCCGCGGCTGGTG
GCGGCGTTGCGGGACCGGGCCGTGGTCCTCGAGGTCTGCCCCACCAGCAACCTGCGTACG
GGCGTGGTCTCCGAGCCGGGGGCGCACCCGCTGCGCCGGTTGTGGGACGCCGGGGTGCGG
CTCACCCTCAACACCGACGACCCCAGCATGTTCCACACCGACCTGCTCGCCGAGCACCGG
TTCGCTGTCGAGTGGCACGGTTTCACCGTGGCCGAACTCGCCGCCATGACGCTGATCGGG
GTCGACGCCGCGCTGCTGCCCGCGACGGAGCGCGAGGCGCTGCGCGAGCGGGTGACGACC
GAGCTGGCGGAGCTGGGCGTGACCGCCGCCGGCCTGCCCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001365 Adenosine/AMP deaminase domain (Domain)
[18-336] 3.30000000000002e-65 PF00962
PF00962   A_deaminase
IPR006330 Adenosine/adenine deaminase (Family)
[18-338] 3.99999999999998e-79 TIGR01430
TIGR01430   Aden_deam
SignalP No significant hit
TMHMM No significant hit
Calm_00150 Calm_00150   Calm_00170 Calm_00170
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