Calm_00160 Calm_00160   Calm_00180 Calm_00180

Calm_00170 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction16,249 / 16,962 / + [in whole cluster]
16,249 / 16,962 / + [in contig]
Location16249..16962 [in whole cluster]
16249..16962 [in contig]
TypeCDS
Length714 bp (237 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative HxlR family transcriptional regulator
Product (GenBank)CalU8
Gene
Gene (GenBank)calU8
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
comment
calicheamicin生合成遺伝子クラスターの報告

このORFは、Fig2の生合成clusterにて、unknown function(U1-U22)として示されているのみ。
本文中、特にふれていない。
Related Reference
ACC
Q8GMD6
NITE
C1027_00570
PmId
[21250756] Improvement of the enediyne antitumor antibiotic C-1027 production by manipulating its biosynthetic pathway regulation in Streptomyces globisporus. (J Nat Prod. , 2011)
comment
Streptomyces globisporus
Putative uncharacterized protein_sgcE1
[40%id, 2e-12]

[PMID:21250756]
SgcR, SgcEの機能解析

putative DNA-binding proteinsと推定されるsgcE1,sgcRの不活性化株作製。sgcE1はC-1027の生合成に影響を与えなかったがsgcR不活性化株はC-1027とheptaeneのtitersが著しく増加した。S.globisporus wild-typeとsgcE1の不活性化株間でassayに変化は見られないことから、SgcE1はC-1027 biosynthesisに必須ではないことを示した。enediyne core biosynthesis geneだけでなくregulatory geneでもないことを示した。sgcE1の機能は明らかになっていない。
Family/Domain
FD
IPR002577
comment
[IPR002577] Helix-turn-helix, HxlR type (Domain)

close this sectionSequence

selected fasta
>putative HxlR family transcriptional regulator [CalU8]
MKEQRDRRTYGQQCGLAFALDVVGERWTLLIIRELLVRPRRYRDLLDALPGIGTNLLADR
LAFLTEAGIIEPLDAERRTAGYALTALGEKLREPVLVLARFGLTLLAEQPARTSASVTRA
SWAVLAIESMVDPARALADEVYEFDIEGELFHVRVAAGEARTVQGTAGADATLRVATDAR
TFFDLGSGAIEPVEAVVSGAVSVTGPPAALPRCLYLLGLGGRPAGERALSAAGAGRR
selected fasta
>putative HxlR family transcriptional regulator [CalU8]
GTGAAAGAGCAACGGGACCGTCGGACGTACGGCCAGCAGTGCGGCCTGGCCTTCGCACTG
GACGTGGTGGGTGAGCGCTGGACGCTGCTCATCATCCGCGAGCTGCTGGTCCGGCCTCGC
CGCTACCGCGACCTGCTGGACGCCCTGCCGGGCATCGGCACCAACCTCCTCGCCGACCGG
CTGGCCTTCCTCACCGAGGCCGGCATCATCGAGCCGCTGGACGCCGAACGGCGCACGGCG
GGCTACGCGCTCACCGCGCTGGGCGAGAAGCTGCGGGAGCCGGTCCTGGTGCTGGCCCGG
TTCGGGCTGACGCTGCTCGCCGAGCAGCCCGCCCGCACCAGCGCCTCGGTGACCCGGGCC
TCCTGGGCCGTGCTGGCCATCGAGTCCATGGTCGACCCGGCCCGGGCCCTCGCCGACGAG
GTCTACGAGTTCGACATCGAGGGCGAGCTGTTCCACGTCCGCGTCGCCGCCGGGGAGGCC
CGCACGGTGCAGGGGACGGCCGGCGCGGACGCCACGTTGCGGGTCGCCACCGACGCCCGG
ACCTTCTTCGATCTCGGCAGCGGCGCGATCGAGCCGGTCGAGGCCGTGGTCAGCGGGGCG
GTCTCCGTCACCGGTCCGCCGGCGGCGCTCCCCCGCTGCCTCTACCTGCTCGGCCTGGGC
GGCCGGCCGGCCGGCGAGCGGGCACTCTCCGCGGCGGGCGCCGGCCGGCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002577 Helix-turn-helix, HxlR type (Domain)
[22-104] 1e-13 PF01638
PF01638   HxlR
[14-110] PS51118
PS51118   HTH_HXLR
IPR003033 SCP2 sterol-binding domain (Domain)
[134-207] 1.9e-05 PF02036
PF02036   SCP2
[118-218] 6.20000124972815e-11 SSF55718
SSF55718   SCP2
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[14-96] 3.2e-05 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
SignalP No significant hit
TMHMM No significant hit
Calm_00160 Calm_00160   Calm_00180 Calm_00180
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