Calm_00310 : CDS information

Location

Organism	Micromonospora echinospora
Strain	NRRL 15839
Entry name	Calicheamicin
Contig	AF497482
Start / Stop / Direction	35,580 / 34,687 / - [in whole cluster] 35,580 / 34,687 / - [in contig]
Location	complement(34687..35580) [in whole cluster] complement(34687..35580) [in contig]
Type	CDS
Length	894 bp (297 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	glucose-1-phosphate nucleotidyltransferase
Product (GenBank)	CalS7
Gene
Gene (GenBank)	calS7
EC number	2.7.7.-
Keyword	aminodideoxypentose
Note
Note (GenBank)
Reference	ACC Q8KNG6 PmId [12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002) [18807197] Cloning and characterization of CalS7 from Micromonospora echinospora sp. calichensis as a glucose-1-phosphate nucleotidyltransferase. (Biotechnol Lett. , 2009) comment [PMID:12183629] calicheamicin生合成遺伝子クラスターの報告このORFは、Fig2の生合成clusterにexpected production of four unusual activated nucleotide sugars(S1 to S14)として示されているのみで、本文中特に触れていない。 [PMID:18807197] abstract only CalS7の機能解析論文大腸菌で発現、精製した。recombinant酵素は有意な thymidylyltransferase activityとuridylyltransferase activityを示したが、cytidyltransferase activityとadenyltransferase activityは無視できる程度であった。

Sequence

>glucose-1-phosphate nucleotidyltransferase [CalS7]
MRGVLLAGGTGSRMWPVTRAVSKQLMPVYDKPMIFYPLSTLVTAGVREILIITRPDEQPL
FRRLLGDGGQWGLDLRYAVQERPDGIAHALLLAADFLAGGPAVLILGDNIIHSAGLDRQL
AELTEVDGGLVFGLPVADPRPYGVLDFDECGAVRDIVEKPLVPPSRYAVPGLYVYGADVV
SIAAELTPSARGELEITDVNREYLRRGRLEVRLLGRRTAWLDTGRFGDLMKAAEYVRVIE
ERHGVKVGCVEEAAWRAGLLDDAAMRRLAVPLRASGYGDYLLRLLDGERPARPAAVG

>glucose-1-phosphate nucleotidyltransferase [CalS7]
ATGCGTGGTGTTTTGCTCGCTGGTGGCACCGGGTCGCGGATGTGGCCGGTGACCCGGGCG
GTCTCCAAGCAACTGATGCCGGTCTACGACAAGCCGATGATCTTCTATCCGCTGAGCACC
CTCGTGACGGCCGGTGTGCGGGAGATCCTCATCATCACGCGCCCCGACGAGCAGCCGCTG
TTCCGGCGCCTGCTCGGCGACGGTGGCCAGTGGGGGCTCGACCTGCGGTACGCCGTGCAG
GAGCGCCCGGACGGCATCGCCCACGCGCTCCTGCTCGCCGCGGACTTCCTCGCCGGCGGC
CCGGCCGTGCTCATCCTCGGCGACAACATCATTCACTCCGCCGGGCTGGACCGGCAGCTC
GCGGAGCTGACCGAGGTGGACGGTGGGCTGGTCTTCGGGCTCCCGGTGGCGGACCCTCGG
CCGTACGGGGTGCTCGACTTCGACGAGTGCGGCGCGGTGCGCGACATCGTGGAGAAGCCC
CTGGTCCCGCCGTCCCGGTACGCCGTGCCCGGCCTCTACGTGTACGGCGCGGACGTCGTC
TCCATCGCCGCCGAACTGACGCCCAGCGCCCGGGGCGAGTTGGAGATCACCGACGTCAAC
CGGGAGTACCTGCGCCGGGGCCGGCTGGAGGTGCGGCTGCTCGGGCGGCGCACCGCGTGG
CTGGACACCGGCCGGTTCGGCGACCTGATGAAGGCGGCCGAGTACGTGCGCGTGATCGAG
GAGCGGCACGGAGTCAAGGTCGGCTGCGTCGAGGAGGCGGCCTGGCGGGCCGGGCTGCTC
GACGACGCGGCGATGCGCCGCCTGGCGGTGCCGCTGCGCGCCAGCGGGTACGGCGACTAC
CTGCTGCGCCTGCTCGACGGCGAGCGCCCGGCGCGCCCGGCCGCCGTCGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family) TIGR01207 RmlA
SignalP	No significant hit
TMHMM	No significant hit

Calm_00300 Calm_00320