Calm_00390 Calm_00390   Calm_00410 Calm_00410

Calm_00400 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction48,717 / 49,559 / + [in whole cluster]
48,717 / 49,559 / + [in contig]
Location48717..49559 [in whole cluster]
48717..49559 [in contig]
TypeCDS
Length843 bp (280 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative AraC family transcriptional regulator
Product (GenBank)CalR7
Gene
Gene (GenBank)calR7
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
comment
calicheamicin生合成遺伝子クラスターの報告

このORFは、Fig2の生合成clusterにてputative regulatory elements(R1-R9)と示されているのみ。
本文中、特にふれていない。
Related Reference
ACC
Q8GME4
NITE
C1027_00490
PmId
[19159491] Role of sgcR3 in positive regulation of enediyne antibiotic C-1027 production of Streptomyces globisporus C-1027. (BMC Microbiol. , 2009)
[20551990] Manipulation of pathway regulation in Streptomyces globisporus for overproduction of the enediyne antitumor antibiotic C-1027. (J Antibiot (Tokyo). , 2010)
comment
Streptomyces globisporus
Putative regulatory protein

[PMID:19159491]
C-1027の生合成遺伝子SgcR2。
制御遺伝子として同定されたSgcR1,R2,R3のSgcR3に関しての機能解析論文。

sgcR3過剰発現株はC-1027の産生能が上がり、sgcR3欠損株はC-1027が産生されなかった。
また、sgcR3欠損株の転写解析ではsgcA1やsgcC4のような生合成構造遺伝子だけでなく、制御遺伝子sgcR1,sgcR2の転写量も減少した。cross-complementation studyにて、sgcR3がsgcR1R2のプロモーター領域に直接結合することでsgcR1とsgcR2の転写を活性化した。


[PMID:20551990]
SgcR1,SgcR2の機能解析論文。

sgcR1,sgcR2,sgcR3の過剰発現株を各作製し、これらの株におけるC-1027とheptaene産生の変化および生物活性を見ている。
C-1027の生産にpositive activatorとして機能していることが示された。
Family/Domain
FD
IPR018060
comment
[IPR018060] DNA binding HTH domain, AraC-type (Domain)

close this sectionSequence

selected fasta
>putative AraC family transcriptional regulator [CalR7]
MEQSREAAAARAIDYMRRHLSEPLQLADLARVVPFSPFHFHRLFRDVTTMTPARFLAALR
MAEARRMLLHSGLTVTAISGHVGYTSAGTFTTQFSRLVGTSPGHFRQMSRLLAGRPCHVL
AGWLRNAVTEVTRPRLVLHVPESEPGDLVLVGLRADGEAADASTTWAVAADGAQVPVVAR
PGPYQARVVLVRGDSTLTRALVDEEPTSHLVGTAELVLPQDGCAAVPVTTAPPRPTDPPA
LALGPVCRLVETFTRLAGPSGRPGPAWSAGRTALAAAAIA
selected fasta
>putative AraC family transcriptional regulator [CalR7]
ATGGAGCAGTCCCGGGAGGCGGCTGCGGCGCGGGCCATCGACTACATGCGCCGGCACCTG
TCGGAGCCGCTGCAGCTGGCCGACCTGGCCCGGGTTGTCCCCTTCAGCCCGTTCCACTTC
CACCGGCTGTTCCGCGACGTGACCACGATGACCCCGGCCCGCTTCCTCGCCGCGCTGCGG
ATGGCGGAGGCCCGCCGGATGCTGCTGCACTCCGGCCTGACGGTGACCGCGATCAGCGGC
CACGTCGGCTACACGAGCGCGGGGACCTTCACCACCCAGTTCTCCCGGCTGGTCGGCACG
TCGCCGGGGCACTTCCGGCAGATGTCCCGGCTGCTGGCCGGCCGGCCCTGCCACGTCCTG
GCGGGCTGGCTGCGCAACGCCGTCACGGAGGTCACCCGACCCCGGCTGGTGCTGCACGTG
CCCGAGAGCGAGCCCGGCGACCTGGTGCTGGTCGGCCTGCGGGCCGACGGGGAGGCCGCG
GACGCGTCGACCACGTGGGCGGTGGCGGCCGACGGCGCGCAGGTCCCGGTGGTGGCGCGG
CCGGGCCCGTACCAGGCCCGGGTCGTGCTGGTCCGGGGCGACAGCACGCTGACCCGCGCC
CTGGTGGACGAGGAGCCCACCAGCCATCTGGTCGGCACCGCCGAACTGGTGCTGCCCCAG
GACGGCTGCGCGGCCGTCCCGGTCACCACCGCGCCGCCGCGGCCGACCGACCCGCCGGCG
CTGGCCCTCGGCCCGGTGTGCCGGCTCGTCGAGACGTTCACGCGGCTGGCCGGCCCGTCG
GGCCGGCCGGGTCCGGCCTGGTCGGCCGGCCGCACCGCGCTGGCCGCGGCGGCCATCGCG
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009057 Homeodomain-like (Domain)
[4-59] 6.90000000000001e-24 G3DSA:1.10.10.60 [60-109] 2.1e-17 G3DSA:1.10.10.60
G3DSA:1.10.10.60   Homeodomain-rel
[5-56] 3.7999986264479e-12 SSF46689 [59-107] 2.00000075217457e-11 SSF46689
SSF46689   Homeodomain_like
IPR018060 DNA binding HTH domain, AraC-type (Domain)
[10-108] PS01124
PS01124   HTH_ARAC_FAMILY_2
[23-106] 3.0999994589937e-27 SM00342
SM00342   HTH_ARAC
SignalP No significant hit
TMHMM No significant hit
Calm_00390 Calm_00390   Calm_00410 Calm_00410
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