Calm_00540 : CDS information

Location

Organism	Micromonospora echinospora
Strain	NRRL 15839
Entry name	Calicheamicin
Contig	AF497482
Start / Stop / Direction	64,685 / 63,477 / - [in whole cluster] 64,685 / 63,477 / - [in contig]
Location	complement(63477..64685) [in whole cluster] complement(63477..64685) [in contig]
Type	CDS
Length	1,209 bp (402 aa)

Annotation

Category	5.1 general function
Product	putative transposase
Product (GenBank)	IS
Gene
Gene (GenBank)	IS
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q8KNE3 PmId [12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002) comment calicheamicin生合成遺伝子クラスターの報告このORFは、Fig2の生合成clusterにて、ISと示されているのみ。本文中では特にふれていない。
Family/Domain	FD IPR001959, IPR010095, IPR021027 comment [IPR001959] Transposase, probable, IS891/IS1136/IS1341 (Domain) [IPR010095] Transposase IS605, OrfB, C-terminal (Domain) [IPR021027] Transposase, putative, helix-turn-helix domain (Domain)

Sequence

>putative transposase [IS]
MRTAYRCRAYPTPEQAAVLNRTFGCVRVVWNRTLAARHARWHTERRSTSYAETDRALTEM
KKQPDLAYLGEVSSVPLQQTLRHQHTAMTAFFAKRARYPRYKSRHGRQSASYTRSAFRLR
SGNLSLGKTPGVLRFVWSLPNVDPAGLDPTMVTVSRDPDGRWFVTFAVDVDTPAAPEPTG
EAVGVDLGLTDFAVLSTGERIPHPKHMERRERRLKRYQRIMARRQKGSANRAKAKRMVAR
AHSQVRDARRDFLHQQSTALVRRFAAIAVEDLAVANMVRNHSLAKAISRTGWAEFRTLLT
YKAHRDGRTLAVVDRWYPSSKTCSACGHLLATLSLGTRHWTCPSCGTRHDRDVNAAKNIT
VAAGLAETKNACGADVRHEGPPSVRSAVNQEPQPARVGLPRL

>putative transposase [IS]
GTGCGGACGGCGTACAGGTGTCGGGCGTACCCGACCCCGGAGCAGGCGGCGGTGCTGAAC
CGCACGTTCGGGTGTGTACGTGTCGTCTGGAACCGCACGCTCGCCGCCCGTCACGCCCGC
TGGCACACCGAACGGAGATCCACCTCCTACGCGGAGACCGATCGGGCGTTGACGGAGATG
AAGAAGCAGCCCGACCTGGCGTATCTGGGTGAGGTGTCGTCGGTGCCGTTGCAGCAGACC
CTGCGGCACCAGCACACCGCCATGACCGCCTTCTTCGCCAAGCGGGCCCGGTACCCGCGC
TACAAGTCCCGACACGGCCGCCAGTCCGCCAGTTACACCCGGTCGGCGTTCCGCCTCCGC
AGCGGGAACCTGTCGTTGGGAAAGACACCCGGGGTGCTGCGGTTCGTGTGGTCGTTGCCG
AACGTGGATCCGGCGGGGTTGGATCCGACGATGGTGACGGTGTCGCGTGACCCGGACGGC
CGCTGGTTCGTGACCTTCGCCGTCGACGTCGACACACCCGCCGCACCCGAACCCACCGGC
GAGGCGGTGGGCGTGGACCTCGGCCTGACCGACTTCGCGGTGCTGTCCACCGGCGAACGC
ATCCCCCACCCGAAGCACATGGAACGGCGGGAACGCCGACTCAAGCGGTATCAGCGGATA
ATGGCCCGCCGACAGAAGGGCTCCGCCAACCGCGCCAAAGCCAAACGAATGGTCGCCCGA
GCGCATTCGCAGGTGCGCGACGCCCGCCGGGACTTCCTCCACCAGCAGTCCACCGCCCTG
GTGCGCCGGTTCGCCGCCATCGCCGTGGAGGACCTGGCCGTGGCGAACATGGTCCGCAAC
CACTCCCTGGCCAAAGCGATCTCGCGTACCGGCTGGGCCGAGTTCCGCACCCTGCTCACC
TACAAGGCGCACCGCGACGGCCGCACCCTCGCGGTGGTGGACCGCTGGTACCCGTCGTCG
AAGACCTGCTCGGCGTGCGGGCACCTGCTCGCCACACTCTCCCTCGGCACACGCCACTGG
ACGTGTCCGAGTTGCGGCACCCGGCATGACCGGGACGTCAACGCAGCCAAGAACATCACC
GTCGCGGCCGGGCTGGCCGAGACGAAAAACGCCTGCGGAGCGGACGTCAGACACGAAGGA
CCTCCTTCCGTGCGGTCTGCGGTGAACCAGGAACCCCAACCCGCGAGGGTCGGACTCCCC
CGCCTTTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001959 Transposase, probable, IS891/IS1136/IS1341 (Domain) PF01385 OrfB_IS605 IPR010095 Transposase IS605, OrfB, C-terminal (Domain) PF07282 OrfB_Zn_ribbon TIGR01766 TspaseT_teng_C IPR021027 Transposase, putative, helix-turn-helix domain (Domain) PF12323 HTH_OrfB_IS605
SignalP	No significant hit
TMHMM	No significant hit

Calm_00530 Calm_00550