Calm_00650 : CDS information

Location

Organism	Micromonospora echinospora
Strain	NRRL 15839
Entry name	Calicheamicin
Contig	AF497482
Start / Stop / Direction	80,077 / 81,147 / + [in whole cluster] 80,077 / 81,147 / + [in contig]
Location	80077..81147 [in whole cluster] 80077..81147 [in contig]
Type	CDS
Length	1,071 bp (356 aa)

Annotation

Category	3.2 modification methylation
Product	C-2 O-methyltransferase
Product (GenBank)	CalO6
Gene
Gene (GenBank)	calO6
EC number	2.1.1.-
Keyword	orsellinic acid moiety
Note
Note (GenBank)
Reference	ACC Q8KND2 PmId [12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002) [21358050] Structural characterization of CalO1: a putative orsellinic acid methyltransferase in the calicheamicin-biosynthetic pathway. (Acta Crystallogr D Biol Crystallogr. , 2011) [24978950] Characterization of the calicheamicin orsellinate C2-O-methyltransferase CalO6. (Chembiochem. , 2014) comment [PMID:12183629](2002) calicheamicin生合成遺伝子クラスターの報告このORFは、Fig2の生合成clusterにて、CalO1-CalO6がorsellinic acid 生合成遺伝子と示されているのみ。本文中では特にふれていない。 ----- [PMID:21358050](2011) CalO1の機能解析 cofactor(S-adenosylhomocysteine)に結合したCalO1を構造解析(2.4 Å resolution)。CalO1とCalO6の立体構造比較を行っている。CalO1とCalO6基質は似たような分子構造を共有していると思われ、基質結合領域のいくつかの残基の違いが基質再配向に影響を与え、その結果2つの酵素間で位置特異性の違いを生むと推定される。 CalO6はin vitroでN-acetyl cysteaminyl orsellinic acid(SNAc-orsellinic acid)のC2 OHの効率的なメチル化を証明した。(S. Singh & J. S. Thorson, unpublished work)。 ----- [PMID: 24978950](2014) abstract calicheamicin生合成に関連したputative orsellinic acid C2-O-methyltransferaseの機能的特徴づけ。この調査から、C2-O-methylationはC3-hydroxylation/methylation と C5-iodinationより前に起こり、CoAまたはACPが結合した基質を必要とすることが示唆された。

Sequence

>C-2 O-methyltransferase [CalO6]
MELTTTAARPGLRHRMQQLIYGFFTAQTLHVAVRLRIPDLLADGARDVGDLASATGADAP
SLRRLLRALVFLEVLDEPAPGTFALTEQGEVLRADVTGSMRELVLLLSGPESWAAWGQLE
HSVRTGEVAWEHVHGRSCFDHLMADPQRQAAFNAAMAEGSRAFVPTLLSAYDFGDLRTVV
DVGGGSGALLAGVLAAHPHLRGTVFDTPDGVADAARTVAEQGVADRCGVETGDFFVSVPP
GADAYVLKSVLHDWDDEQCVEVLRTVRRAVRPDSRVILVESLMPTTVTTAPSVAQVVMND
LNMMVCHGGRERTVAEFRELLRVAGFRLESVTPCPAPSVVGILEAAPAPATGPDGS

>C-2 O-methyltransferase [CalO6]
ATGGAACTCACCACGACCGCCGCCCGGCCCGGGCTGCGGCACCGGATGCAACAGCTGATC
TACGGGTTCTTCACGGCGCAGACGCTGCACGTGGCGGTCCGGCTGCGGATACCGGACCTG
CTCGCCGACGGCGCGCGCGACGTCGGCGACCTCGCGTCGGCCACCGGCGCGGACGCCCCG
TCGCTGCGCCGGCTGCTGCGGGCGCTGGTCTTCCTGGAGGTGCTCGACGAGCCCGCGCCA
GGCACCTTCGCCCTCACCGAACAGGGCGAGGTGCTGCGGGCGGACGTGACCGGGTCGATG
CGGGAGCTGGTGCTGCTGCTGTCCGGGCCGGAGAGCTGGGCCGCGTGGGGGCAGCTCGAA
CACAGCGTGCGCACCGGCGAGGTGGCGTGGGAGCACGTGCACGGCCGGAGCTGCTTCGAC
CACCTGATGGCGGACCCGCAGCGGCAGGCGGCGTTCAACGCGGCCATGGCCGAGGGCTCG
CGGGCCTTCGTGCCCACGCTGCTGTCGGCGTACGACTTCGGCGACCTGCGCACCGTGGTG
GACGTGGGCGGCGGCAGCGGCGCCCTGCTCGCCGGGGTCCTCGCGGCGCACCCGCACCTG
CGCGGCACGGTCTTCGACACCCCGGACGGCGTGGCCGACGCGGCGCGCACGGTCGCCGAG
CAGGGCGTCGCCGACCGGTGCGGGGTGGAGACCGGTGACTTCTTCGTCTCCGTGCCACCG
GGCGCGGACGCCTACGTGCTCAAGAGCGTGCTGCACGACTGGGACGACGAGCAGTGCGTC
GAGGTGCTGCGCACCGTCCGCCGGGCGGTCCGACCCGACAGCCGCGTGATCCTCGTCGAG
TCGCTGATGCCGACGACGGTCACCACGGCGCCGAGCGTCGCCCAGGTGGTCATGAACGAC
CTCAACATGATGGTCTGCCACGGCGGCCGGGAGCGCACCGTGGCGGAGTTCCGCGAGTTG
CTGCGGGTGGCGGGCTTCCGGCTGGAGTCCGTCACGCCCTGCCCGGCGCCGAGCGTCGTC
GGCATCCTGGAGGCGGCGCCCGCCCCGGCCACCGGGCCGGACGGGAGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001077 O-methyltransferase, family 2 (Domain) PF00891 Methyltransf_2 IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016461 O-methyltransferase, caffeic acid-type (Family) PIRSF005739 O-mtase
SignalP	No significant hit
TMHMM	No significant hit

Calm_00640 Calm_00660