Calm_00680 Calm_00680   Calm_00700 Calm_00700

Calm_00690 : CDS information

close this sectionLocation

Organism
StrainNRRL 15839
Entry nameCalicheamicin
Contig
Start / Stop / Direction84,045 / 85,229 / + [in whole cluster]
84,045 / 85,229 / + [in contig]
Location84045..85229 [in whole cluster]
84045..85229 [in contig]
TypeCDS
Length1,185 bp (394 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductNDP-aminosugar N-oxidase
Product (GenBank)CalE10
Gene
Gene (GenBank)calE10
EC number
Keyword
  • 4-hydroxyamino-6-deoxy-alpha-D-glucose
Note
Note (GenBank)
Reference
ACC
PmId
[12183629] The calicheamicin gene cluster and its iterative type I enediyne PKS. (Science. , 2002)
[19055330] Characterization of CalE10, the N-oxidase involved in calicheamicin hydroxyaminosugar formation. (J Am Chem Soc. , 2008)
comment
[PMID:12183629]
calicheamicin生合成遺伝子クラスターの報告

このORFは、Fig2の生合成clusterにて、polyketide construction or modification (01 to O6 and E1 to E10)と示されているのみ。本文中では特にふれていない。


[PMID:19055330]
CalE10の機能解析

Streptomyces lividansで発現・精製、酵素学的解析を行っている。NDP-aminosugar N-oxidaseに要求されることを証明。その酸化はグリコシル転移に先立って糖ヌクレオチド段階で生じることも確かめた。

close this sectionSequence

selected fasta
>NDP-aminosugar N-oxidase [CalE10]
MPRRCPFGPPAEYARLRTERPVARLPMLGGNTAWVVSRYADVKRVLSDPRMSADRRRAGF
PRFAPTTESQRQASFANFRPPLNWMDPPEHTAARRQIVDEFAARRVRQLRPLVERVVDEH
LDAMTAGRSSADLVPSFSYPVPSRVICEMLGVPYGEHAFFERRSTRMLSRGVPADERARC
AREIREFLDGVVTDKERHPGDDVLSRLLAAQRAAGEPDHEAVVSMAFVLLVAGHVTTSNM
ISLSVLALLTHPERLARLRAEPDRFPAAVEELLRYFTIVEAATARTATADVTVGGVTIRA
GEGVVALGQAANRDPAAFDRPDEFDPDRDARHHLAFGYGRHICPGQHLARLELDVALSRL
VRRLPGLRLTVDVDDLPLKEDGNIFGLHALPVAW
selected fasta
>NDP-aminosugar N-oxidase [CalE10]
CTGCCCCGCCGGTGCCCGTTCGGCCCGCCGGCCGAGTACGCCCGGCTGCGGACCGAGCGG
CCGGTCGCCCGGCTGCCCATGCTCGGCGGCAACACGGCCTGGGTGGTGTCCCGGTACGCC
GACGTCAAGCGGGTGCTCAGCGACCCGCGGATGAGCGCGGACCGGCGCCGGGCCGGTTTT
CCGCGGTTCGCGCCGACCACCGAGAGCCAGCGGCAGGCCTCGTTCGCGAACTTCCGCCCC
CCGCTGAACTGGATGGACCCGCCGGAGCACACCGCCGCCCGCCGCCAGATCGTCGACGAG
TTCGCCGCGCGGCGGGTACGACAGCTGCGGCCGCTGGTCGAGCGGGTGGTGGACGAGCAC
CTCGACGCCATGACGGCCGGGCGGTCGAGCGCCGACCTGGTGCCGTCGTTCAGCTATCCG
GTGCCGTCGCGGGTGATCTGCGAGATGCTCGGCGTGCCGTACGGCGAACACGCGTTCTTC
GAGCGCCGGTCCACCCGGATGCTGAGTCGCGGCGTGCCCGCGGACGAGCGGGCCCGGTGC
GCCCGCGAGATCCGCGAGTTCCTCGACGGCGTGGTGACCGACAAGGAGCGGCACCCCGGC
GACGACGTGCTCAGCCGGCTGCTCGCCGCGCAGCGCGCGGCCGGCGAGCCCGACCACGAG
GCCGTGGTGAGCATGGCCTTCGTGCTGCTGGTCGCCGGGCACGTCACGACGTCGAACATG
ATCTCGCTGAGCGTGCTGGCCCTGTTGACCCATCCGGAGCGGCTCGCCCGCCTGCGCGCC
GAGCCGGACCGGTTCCCCGCCGCCGTCGAGGAGCTGCTGCGGTACTTCACCATCGTCGAG
GCGGCGACCGCGCGGACCGCGACCGCCGACGTGACGGTCGGTGGGGTCACCATCCGGGCC
GGGGAGGGGGTGGTGGCGCTGGGCCAGGCCGCCAACCGGGACCCGGCGGCGTTCGACCGG
CCGGACGAGTTCGACCCGGACCGCGACGCCCGGCACCACCTCGCCTTCGGCTACGGACGA
CACATCTGCCCCGGCCAGCACCTGGCCCGGCTGGAACTGGACGTCGCGCTGAGCCGGCTG
GTCCGGCGGCTGCCCGGGCTGCGGTTGACCGTGGACGTGGACGACCTGCCGCTCAAGGAG
GACGGCAACATCTTCGGCCTGCACGCGCTGCCGGTCGCCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[7-394] 2.30000000000004e-111 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[232-249] 3.79999862644791e-08 PR00385 [267-278] 3.79999862644791e-08 PR00385 [334-343] 3.79999862644791e-08 PR00385 [343-354] 3.79999862644791e-08 PR00385
PR00385   P450
[2-394] 4.99996518094122e-101 SSF48264
SSF48264   Cytochrome_P450
[180-364] 4.9e-30 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
[85-96] 1.40000506907309e-52 PR00359 [132-148] 1.40000506907309e-52 PR00359 [149-164] 1.40000506907309e-52 PR00359 [186-208] 1.40000506907309e-52 PR00359 [267-278] 1.40000506907309e-52 PR00359 [285-312] 1.40000506907309e-52 PR00359 [313-328] 1.40000506907309e-52 PR00359 [334-343] 1.40000506907309e-52 PR00359 [343-354] 1.40000506907309e-52 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[336-345] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Calm_00680 Calm_00680   Calm_00700 Calm_00700
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