Dynm_00080 Dynm_00080   Dynm_00100 Dynm_00100

Dynm_00090 : CDS information

close this sectionLocation

Organism
StrainATCC 53710 (=NBRC 15963)
Entry nameDynemicin
Contig
Start / Stop / Direction15,598 / 16,656 / + [in whole cluster]
15,598 / 16,656 / + [in contig]
Location15598..16656 [in whole cluster]
15598..16656 [in contig]
TypeCDS
Length1,059 bp (352 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative tryptophanyl-tRNA synthetase
Product (GenBank)putative tryptophanyl-tRNA synthetase
Geneorf10
trpS2
Gene (GenBank)
EC number6.1.1.2
Keyword
Note
Note (GenBank)
  • ORF10
Reference
ACC
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
comment
Dynemicin生合成遺伝子クラスターの報告。

orf10 Tryptophanyl tRNA synthetase

機能解析はされていない。
Related Reference
ACC
Q9RVD6
PmId
comment
Deinococcus radiodurans_trpS2(trpSII)
Tryptophanyl-tRNA synthetase 2

D. radioduransでのTrpSIIに関する解析。 D.radioduransでもTrpS遺伝子は2つ持っているが、TrpS2の方がNO-synthaseと相互作用することが示された。 In vitroでも相互作用しNO-synthaseの活性を強めた。 tRNA合成活性もin vitroで確認。

close this sectionSequence

selected fasta
>putative tryptophanyl-tRNA synthetase [putative tryptophanyl-tRNA synthetase]
MSAARMLTGDRPTGRLHLGHYVGSIANRVKLHQHYESFFIIADLHMLTTRNSREDIEQVS
HNAREMVTDILAAGVEPDRATFYLQSAIPEVGDLNTLFQNLVTVPRLERVPSLKEMSRDA
GKEEMPYGLLGYPVLQAADILCVKGQVVPVGKDNAAHVEVTREIARRFNHLYGEVFPVPE
MIMSETPTLVGTDGAGKMSKSKGNAIALSDDPATVRRKVLGMYTDPNRVRADVPGTVEGN
PVFAYLDVFHTDRATVEELKARYRAGRVGDVEVKEELVGALNRFLDPVRERRARFEGQRG
LVDELIVEGTERTRAQVRRTLVEVRRAMGLTSAYTQVRRRAERYRKAVGTPA
selected fasta
>putative tryptophanyl-tRNA synthetase [putative tryptophanyl-tRNA synthetase]
ATGTCCGCAGCACGGATGCTCACCGGTGACCGCCCGACCGGGCGGCTGCACCTCGGCCAC
TACGTCGGCAGCATCGCCAACCGGGTGAAGCTGCACCAGCACTACGAGAGCTTCTTCATC
ATCGCCGACCTGCACATGCTCACCACGCGCAACAGTCGCGAGGACATCGAGCAGGTCTCC
CACAACGCCCGCGAGATGGTCACCGACATCCTCGCCGCCGGTGTCGAACCGGACCGGGCC
ACCTTCTACCTCCAGTCGGCGATCCCCGAGGTCGGCGACCTGAACACCCTGTTCCAGAAC
CTGGTCACCGTGCCCCGGCTGGAACGGGTCCCGTCGCTCAAGGAGATGAGCCGGGACGCC
GGCAAGGAGGAGATGCCGTACGGGCTGCTCGGCTATCCCGTCCTCCAGGCCGCCGACATC
CTCTGCGTGAAGGGGCAGGTCGTCCCGGTGGGGAAGGACAACGCCGCGCACGTCGAGGTG
ACCCGGGAGATCGCCCGGCGCTTCAACCACCTCTACGGCGAGGTCTTCCCCGTGCCGGAA
ATGATCATGTCGGAGACGCCCACCCTGGTCGGCACCGACGGCGCGGGGAAGATGAGCAAG
AGCAAGGGAAACGCCATCGCGCTCTCCGACGACCCGGCCACCGTGCGGCGCAAGGTGCTG
GGGATGTACACCGACCCGAACCGGGTCCGCGCCGACGTGCCGGGCACGGTCGAGGGGAAC
CCGGTCTTCGCGTACCTCGACGTGTTCCACACCGACCGGGCGACGGTCGAGGAGCTGAAG
GCGCGCTACCGGGCCGGCCGGGTCGGCGACGTCGAGGTCAAGGAGGAACTGGTGGGCGCG
CTCAACCGGTTCCTCGACCCGGTCCGCGAGCGGCGGGCCCGGTTCGAGGGGCAGCGCGGC
CTGGTCGACGAGCTGATCGTCGAGGGCACCGAGCGGACCCGGGCGCAGGTGCGCCGCACC
CTTGTCGAGGTGCGCCGGGCCATGGGCCTGACCAGCGCGTACACCCAGGTGCGGCGGCGG
GCCGAGCGCTACCGCAAGGCCGTCGGCACGCCGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001412 Aminoacyl-tRNA synthetase, class I, conserved site (Conserved_site)
[12-21] PS00178
PS00178   AA_TRNA_LIGASE_I
IPR002305 Aminoacyl-tRNA synthetase, class Ic (Family)
[4-285] 1.39999999999997e-72 PF00579
PF00579   tRNA-synt_1b
IPR002306 Tryptophanyl-tRNA synthetase (Family)
[16-32] 3.39999972437717e-23 PR01039 [69-88] 3.39999972437717e-23 PR01039 [146-167] 3.39999972437717e-23 PR01039 [197-207] 3.39999972437717e-23 PR01039
PR01039   TRNASYNTHTRP
[4-330] 2.20000000000002e-87 TIGR00233
TIGR00233   TrpS
IPR014729 Rossmann-like alpha/beta/alpha sandwich fold (Domain)
[5-205] 3.19999999999996e-74 G3DSA:3.40.50.620
G3DSA:3.40.50.620   Rossmann-like_a/b/a_fold
SignalP No significant hit
TMHMM No significant hit
Dynm_00080 Dynm_00080   Dynm_00100 Dynm_00100
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