Dynm_00220 Dynm_00220   Dynm_00240 Dynm_00240

Dynm_00230 : CDS information

close this sectionLocation

Organism
StrainATCC 53710 (=NBRC 15963)
Entry nameDynemicin
Contig
Start / Stop / Direction37,166 / 35,283 / - [in whole cluster]
37,166 / 35,283 / - [in contig]
Locationcomplement(35283..37166) [in whole cluster]
complement(35283..37166) [in contig]
TypeCDS
Length1,884 bp (627 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative transcriptional regulator
Product (GenBank)DynR2
GenedynR2
Gene (GenBank)
EC number
Keyword
  • SARP family
Note
Note (GenBank)
  • putative transcriptional regulator; CalR2-like protein
Reference
ACC
PmId
[18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008)
comment
Dynemicin生合成遺伝子クラスターの報告。

dynR2 Putative transcriptional regulator

機能解析はされていない。
Related Reference
ACC
Q2MGB3
NITE
Fred_00260
PmId
[18556785] Identification and utility of FdmR1 as a Streptomyces antibiotic regulatory protein activator for fredericamycin production in Streptomyces griseus ATCC 49344 and heterologous hosts. (J Bacteriol. , 2008)
comment
Streptomyces griseus
Regulatory protein

FdmR1はStreptomyces antibiotic regulatory protein (SARP) familyのメンバーに高い類似性を示す。
fdmR1不活化でfredericamycin (FDM)生合成不可、相補で回復。

RT-PCRによる転写解析で、fdm gene cluster 28genesのうちfdmRとfdmT2以外の26genesが、FdmR1のpositive control下にあることが判明。
fdmR1過剰発現でFDM力価は5.6倍に改善。

fdm clusterを異種性に発現した時、S. albusではFDM産生可能だが、S. lividansではできない。
しかし、fdmR1を恒常的に発現するとFDM産生をもたらすことができる。そしてputative ketoreductaseをコードするfdmCを、fdmR1と同時に過剰発現することによってさらに拡大される。

close this sectionSequence

selected fasta
>putative transcriptional regulator [DynR2]
MGAAVDTVRIKVLGPVAAFRDTTEIPLGPSRQRAVLGYLALHPGQPVPVGALTEAVWGDD
APASGDQLVRTYVARLRQVLEPDTAPRARTNVIASVPGGYRLDVPPDRVDASHFQQLLGR
ARARQAAGAPHEAFELLDAALGLWQDPTLADLSGLLASPRHLTALRADWVDAGLRHVAVG
LSLGQASVVLPVAEQLAAVEPLQEAVQARYLEALTRSGQRAAAVHHYLSVRARLRAELGV
EPGPELRRMYAALLHAGDEPGPGVEPPSRAGADEPDRRPGWRGPGPAIGPLIGREDDVAR
CATLLVAHRAVTIVGPAGCGKSATGLAVAERVAAAFPDGVATVDAAEARDCGEVLRLLAE
TLELPGPNADPLRAVADKRMLLVLNDVEHLGSRAGEMVEHALHRCPRTAMLVTSRRRLGL
AHEAVFRLAPLPLPGTDAPDALPDNPAVRLFVHRAGLVRPGFRLAPADAVTVARICRGLD
GLPLAIELAAACLYTDSLDGVLRRVDDPLDELRPQRRGHPQHHRSLRAALHRSLAALAPV
ERACLARLAAGDRCFSRDEAAARCAGLVPPARLGAVLDRLVDESLLDARPGPGVPRYRLL
RTVALAAATVPESGEHQAFHGMVGNQP
selected fasta
>putative transcriptional regulator [DynR2]
ATGGGTGCTGCCGTGGACACTGTGCGGATAAAGGTGTTGGGCCCGGTCGCCGCGTTCCGT
GACACCACCGAGATCCCCCTGGGGCCGTCCCGGCAGCGTGCGGTCCTCGGCTACCTCGCC
CTGCACCCCGGGCAGCCGGTCCCGGTCGGCGCGCTCACCGAGGCGGTCTGGGGCGACGAC
GCCCCGGCCAGCGGCGACCAGCTCGTCCGGACGTACGTGGCGCGGCTGCGCCAGGTGCTG
GAGCCGGACACCGCGCCGCGCGCCCGGACCAACGTCATCGCCTCGGTGCCCGGCGGCTAC
CGGCTCGACGTGCCGCCGGACCGCGTCGACGCGTCGCACTTCCAGCAGCTGCTCGGCCGG
GCCCGGGCCCGGCAGGCGGCCGGCGCGCCGCACGAGGCCTTCGAGCTGCTCGACGCGGCG
CTCGGGCTCTGGCAGGACCCGACCCTCGCCGACCTCAGTGGCCTGCTGGCCAGCCCCCGG
CACCTCACCGCGCTGCGCGCCGACTGGGTCGATGCCGGCCTGCGCCACGTCGCCGTGGGG
CTGAGCCTCGGCCAGGCGTCGGTGGTGCTCCCGGTGGCCGAGCAGCTCGCGGCCGTCGAG
CCGCTCCAGGAGGCGGTGCAGGCCCGCTACCTGGAGGCGTTGACCCGCAGTGGGCAGCGG
GCCGCCGCCGTGCACCACTACCTGAGCGTCCGGGCCCGGCTCCGCGCCGAACTCGGTGTC
GAGCCCGGTCCGGAGCTGCGCCGGATGTACGCCGCCCTGCTGCACGCCGGGGACGAGCCG
GGGCCGGGCGTCGAACCGCCGTCCCGGGCCGGGGCGGACGAACCCGACCGGCGGCCCGGC
TGGCGCGGACCCGGCCCGGCGATCGGCCCGCTGATCGGCCGGGAGGACGACGTCGCCCGC
TGCGCCACGCTGCTCGTCGCGCACCGGGCCGTCACCATCGTCGGGCCGGCCGGCTGCGGC
AAGTCGGCCACCGGGCTGGCGGTCGCCGAACGCGTCGCCGCCGCGTTCCCCGACGGCGTG
GCCACTGTCGACGCGGCCGAGGCCCGGGACTGCGGCGAGGTGCTGCGGCTGCTGGCCGAG
ACGCTGGAACTGCCGGGGCCGAACGCCGACCCGCTGCGCGCCGTAGCCGACAAGCGGATG
CTGCTGGTGCTCAACGACGTCGAGCACCTCGGCTCGCGGGCCGGCGAGATGGTGGAGCAT
GCCCTGCACCGCTGCCCGCGCACGGCCATGCTGGTCACCTCCCGCCGCCGGCTCGGGCTC
GCCCACGAGGCGGTGTTCCGGCTCGCACCCCTGCCGCTGCCCGGGACCGATGCCCCGGAC
GCGCTGCCGGACAACCCGGCCGTGCGCCTCTTCGTCCACCGGGCCGGGCTGGTCCGGCCC
GGCTTCCGCCTCGCCCCGGCGGACGCGGTGACCGTGGCGCGTATCTGCCGCGGGCTGGAC
GGCCTGCCGCTGGCCATCGAACTGGCCGCCGCCTGCCTCTACACCGACAGCCTCGACGGG
GTGCTGCGCCGGGTCGACGACCCGCTGGACGAGTTGCGGCCACAGCGGCGCGGCCATCCG
CAGCACCACCGTTCGCTGCGCGCCGCGCTGCACCGCAGCCTGGCCGCGCTCGCCCCGGTC
GAGAGGGCCTGCCTGGCCCGCCTGGCCGCCGGTGACCGGTGCTTCTCGCGCGACGAGGCG
GCCGCCCGCTGCGCCGGCCTGGTCCCGCCGGCCCGGCTCGGGGCCGTGCTCGACCGGCTC
GTCGACGAGTCCCTGCTGGACGCCCGGCCCGGGCCGGGCGTCCCGCGGTACCGGCTCCTC
CGCACGGTGGCGCTGGCGGCGGCCACCGTGCCGGAGAGCGGGGAGCATCAGGCGTTCCAC
GGCATGGTCGGGAACCAGCCGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000767 Disease resistance protein (Family)
[310-325] 3.00000067992871e-06 PR00364 [374-388] 3.00000067992871e-06 PR00364 [479-493] 3.00000067992871e-06 PR00364
PR00364   DISEASERSIST
IPR001867 Signal transduction response regulator, C-terminal (Domain)
[30-102] 2.3e-13 PF00486
PF00486   Trans_reg_C
[22-102] 1.20000117458134e-16 SM00862
SM00862   Trans_reg_C
IPR003593 AAA+ ATPase domain (Domain)
[307-435] 6.40000038788486e-06 SM00382
SM00382   AAA
IPR005158 Bacterial transcriptional activator domain (Domain)
[109-254] 6.39998633403535e-34 SM01043
SM01043   BTAD
[109-254] 6.19999999999999e-30 PF03704
PF03704   BTAD
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[19-105] 7.59999999999999e-13 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[7-103] 8.60000664726925e-16 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Dynm_00220 Dynm_00220   Dynm_00240 Dynm_00240
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