Dynm_00300 : CDS information

Location

Organism	Micromonospora chersina
Strain	ATCC 53710 (=NBRC 15963)
Entry name	Dynemicin
Contig	EF552206
Start / Stop / Direction	43,124 / 44,674 / + [in whole cluster] 43,124 / 44,674 / + [in contig]
Location	43124..44674 [in whole cluster] 43124..44674 [in contig]
Type	CDS
Length	1,551 bp (516 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	ketone reductase
Gene	orf17
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF20
Reference	ACC B2BM45 PmId [18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008) comment Dynemicin生合成遺伝子クラスターの報告。 orf17 Ketone reductase genbank: orf20 homologyから機能推測している。PKSのKR domainに似たorf17を持つことは、他のエンジインと比較してユニークであると述べている。機能解析はされていない。
Family/Domain	FD IPR013968 comment [IPR013968] Polyketide synthase, KR (Domain)

Sequence

>putative oxidoreductase [ketone reductase]
MLQDHDRYIFMKLLSGRCPMSPEPLSRPSRIALRALRPAPAPVRPTPRATRWRVTGPADH
PLVRHLRRQPYPAAGPEADVLVLGGRWPAGGAGVLLDAYQRAAGRQLTLVHDGAGGGSLL
RGLGAGDGRPVGTVALGDPTPAALRRAAGLLAAPSAADELTVAADGTVHTLGWRAAPLPG
RAPRFAGATVLVTGGLGGLGSRLAVALAAAGAVPVLLDVRTPDDAPPDVRRMLTLLRRRC
PRARLLTLDLTDPAAARAALAGLRPTAIVHCAGRIAGGTGVRLGPAEVDALVAAKVATLE
TVLATVRAKRLRAVLTFGSLTAHGAHPGLAGYALANELLHRATARYAATHPGVRCCTAEW
SLWSGAGMARQVARVAARHLGMVPVPVATGVAAATRLLAALVDAPPDEDLPDSLLVCSER
PGTQGAWSGRPEGVPGVDAALVVPAGGDLDMAMRAVAEAAAGGAALIPDGTAGPGAPLLV
RATVTGDGVDCVVRSGADPDGPALRSSRYRLLPAGT

>putative oxidoreductase [ketone reductase]
TTGCTTCAAGACCACGATCGATATATCTTTATGAAGTTGCTGAGTGGAAGGTGCCCCATG
TCACCTGAGCCCCTCTCGCGCCCCTCCCGGATCGCCCTGCGCGCCCTGCGGCCCGCGCCG
GCGCCCGTCCGGCCCACCCCCCGCGCGACCCGCTGGCGGGTGACCGGCCCGGCCGACCAC
CCCCTCGTCCGGCACCTGCGCCGGCAGCCCTATCCGGCCGCCGGACCCGAGGCCGACGTG
CTGGTCCTCGGTGGACGGTGGCCGGCCGGCGGCGCCGGGGTGCTGCTCGACGCGTACCAG
CGGGCCGCCGGGCGGCAGCTCACGCTGGTCCACGACGGAGCCGGCGGCGGCAGCCTGCTG
CGCGGGCTCGGCGCCGGGGACGGCCGGCCGGTCGGCACCGTCGCGCTGGGCGACCCCACC
CCGGCGGCGTTGCGCCGCGCCGCCGGCCTGCTCGCCGCGCCGTCCGCCGCCGACGAGCTC
ACCGTGGCCGCCGACGGCACCGTGCACACCCTCGGCTGGCGGGCCGCCCCGCTGCCCGGC
CGGGCGCCACGCTTCGCCGGGGCCACCGTCCTGGTCACCGGCGGCCTGGGCGGGCTCGGC
AGCCGCCTCGCCGTCGCCCTGGCCGCCGCCGGCGCCGTCCCGGTCCTGCTCGACGTGCGT
ACGCCTGACGACGCCCCGCCCGACGTCCGCCGGATGCTCACCCTGCTGCGGCGGCGCTGC
CCCCGGGCCCGGCTGCTCACCCTCGACCTCACCGACCCGGCGGCCGCCCGCGCGGCCCTG
GCCGGGCTGCGGCCCACCGCGATCGTGCACTGCGCCGGCCGGATCGCCGGCGGCACCGGG
GTACGCCTCGGCCCCGCCGAGGTCGACGCCCTGGTCGCCGCGAAGGTCGCCACCCTGGAG
ACGGTGCTCGCCACGGTCCGCGCGAAGCGGCTGCGGGCGGTGCTCACCTTCGGCTCGCTC
ACCGCGCACGGCGCGCACCCGGGGCTCGCCGGCTACGCCCTCGCGAACGAACTGCTGCAC
CGGGCCACCGCCCGGTACGCCGCCACGCACCCCGGGGTCCGCTGCTGCACCGCCGAGTGG
TCACTCTGGTCCGGCGCCGGGATGGCCCGGCAGGTGGCCCGGGTCGCCGCCCGGCACCTC
GGCATGGTGCCGGTGCCGGTGGCCACCGGTGTCGCCGCGGCCACCCGGCTGCTCGCCGCG
CTTGTCGACGCCCCGCCCGACGAGGACCTGCCCGACTCGCTGCTCGTCTGCAGCGAGCGC
CCCGGCACGCAGGGCGCCTGGTCGGGGCGGCCGGAGGGCGTACCCGGAGTGGACGCCGCC
CTGGTCGTGCCGGCCGGCGGCGACCTCGACATGGCGATGCGCGCGGTGGCCGAGGCGGCG
GCCGGCGGCGCCGCGTTGATCCCGGACGGGACGGCCGGTCCGGGCGCGCCGCTGCTGGTC
CGGGCCACGGTCACCGGTGACGGGGTGGACTGCGTGGTCCGCTCCGGCGCCGACCCCGAC
GGGCCGGCACTGCGCAGCTCCCGCTACCGGCTGCTGCCCGCCGGCACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013968 Polyketide synthase, KR (Domain) PF08659 KR IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Dynm_00290 Dynm_00310