Dynm_00450 : CDS information

Location

Organism	Micromonospora chersina
Strain	ATCC 53710 (=NBRC 15963)
Entry name	Dynemicin
Contig	EF552206
Start / Stop / Direction	58,391 / 57,582 / - [in whole cluster] 58,391 / 57,582 / - [in contig]
Location	complement(57582..58391) [in whole cluster] complement(57582..58391) [in contig]
Type	CDS
Length	810 bp (269 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance ABC transporter permease protein
Product (GenBank)	putative integral membrane transport protein
Gene	dynT8
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	ORF34
Reference	ACC B2BM60 PmId [18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008) comment Dynemicin生合成遺伝子クラスターの報告。 dynT8 Putative ABC transporter 機能解析はされていない。
Related Reference	ACC Q53717 PmId [8326867] Streptomyces antibioticus contains at least three oleandomycin-resistance determinants, one of which shows similarity with proteins of the ABC-transporter superfamily. (Mol Microbiol. , 1993) comment Streptomyces antibioticus_oleC-ORF5 Membrane protein S. antibioticsでoleandomycinに抵抗性を示す遺伝子を単離。 OleC plasmidでクローニングされたORFが5つあり、 Orf4 (NBD), Orf5(TMD)がABC transorterとして働いているのではないかと推論。 Orf5(TMD）については後にTopologyの解析もしてABC transporterのPermeaseであるといっている。
Related Reference	ACC P32011 NITE Adria_00140 PmId [1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991) [9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997) [15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005) [19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment Streptomyces peucetius_drrB Daunorubicin/doxorubicin resistance ABC transporter permease protein drrB [PMID: 1924314] DrrAは排出ABC transporterのATP結合タンパク質。Mg2+依存的にATP (or GTP) を結合。 doxorubicin(DXR) or daunorubicin(DNR)の添加で活性が上昇。 DrrBはABC-2 type transporterといわれているもの。 -- さらにいくつかの続報がでており、DrrABはATP依存的なDXRとDNRの排出ポンプとされている(PMID: 15709779)。上記著者らでの最新の続報は[PMID: 19063901] -- 違うグループでも報告あり。上記既報をrefとして採用している。 [PMID: 20158521] drrAB(-)mutantはDNRに高い感受性あり。このmutantはwildと比べて、薬剤産生が10倍減。 key regulator dnrI and polyketide synthase gene dpsAの発現が激減。 regulatory genes dnrO and dnrNの発現は増。これらの結果から細胞内DNR濃度に基づくFeedback regulationを提唱。

Sequence

>putative drug resistance ABC transporter permease protein [putative integral membrane transport protein]
MTAVAAPHPANNARLLPSVGVLTGRALRIYRRTPQLLILRIVQSVAFLLIFRYVFGGAIG
TGTMRYVDFMAPGLLTVGIMFATIGTALGVAEDHVGGLFDRLRSLPMPRSAVLTGRVLAD
LAMTVAVLIPTIAIAFVVGMRVHTDWGSALGALGLLVLFGFAFVWIFVAVGLLAGSVQAA
QGISFIVMPISFASSAFVPTSSMPGWLQAFTEHQPVTVVVNAVRTLTQGAPAEALLGHST
GYYVTRALIWIIALTVVFAGLAIARYRSR

>putative drug resistance ABC transporter permease protein [putative integral membrane transport protein]
ATGACCGCCGTGGCCGCGCCGCACCCGGCCAACAACGCCCGCCTGCTGCCCAGCGTCGGC
GTGCTCACCGGTCGCGCCCTGCGCATCTACCGGCGCACGCCCCAACTGCTGATCCTGCGC
ATCGTGCAGTCGGTGGCGTTCCTGCTGATCTTCCGGTACGTGTTCGGTGGCGCGATCGGC
ACCGGCACGATGCGCTACGTCGACTTCATGGCGCCCGGCCTGCTCACGGTGGGGATCATG
TTCGCCACGATCGGCACCGCGCTCGGCGTGGCCGAGGACCACGTCGGCGGCCTCTTCGAC
CGGCTGCGGTCGCTGCCCATGCCGCGCAGCGCCGTGCTCACCGGCCGGGTCCTCGCCGAC
CTCGCCATGACCGTCGCGGTGCTCATCCCGACCATCGCCATCGCCTTCGTGGTGGGGATG
CGGGTGCACACCGACTGGGGCTCCGCGCTCGGCGCGCTGGGCCTCCTGGTGCTCTTCGGG
TTCGCCTTCGTCTGGATCTTCGTGGCGGTCGGGCTGCTGGCCGGCAGCGTCCAGGCGGCG
CAGGGCATCTCGTTCATCGTGATGCCGATCAGCTTCGCGTCCAGCGCCTTCGTGCCGACC
TCCAGCATGCCGGGCTGGCTCCAGGCGTTCACCGAGCACCAGCCCGTGACGGTGGTGGTC
AACGCGGTCCGCACCCTCACCCAGGGCGCCCCCGCCGAGGCGCTGCTCGGCCACAGCACC
GGCTACTACGTCACCCGGGCGCTGATCTGGATCATCGCACTGACCGTGGTCTTCGCGGGC
CTGGCCATCGCCCGCTACCGGAGCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000412 ABC-2 (Family) PS51012 ABC_TM2 IPR013525 ABC-2 type transporter (Domain) PF01061 ABC2_membrane
SignalP	No significant hit
TMHMM	Transmembrane 6

Dynm_00440 Dynm_00460