Dynm_00460 : CDS information

Location

Organism	Micromonospora chersina
Strain	ATCC 53710 (=NBRC 15963)
Entry name	Dynemicin
Contig	EF552206
Start / Stop / Direction	59,389 / 58,388 / - [in whole cluster] 59,389 / 58,388 / - [in contig]
Location	complement(58388..59389) [in whole cluster] complement(58388..59389) [in contig]
Type	CDS
Length	1,002 bp (333 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance ABC transporter ATP-binding protein
Product (GenBank)	DynT5
Gene	dynT5
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	putative ABC transporter ATP-binding protein; daunorubicin resistance ABC transporter ATPase subunit; similar to CalT5 protein in GenBank Accession Number AAM70341
Reference	ACC B2BM61 PmId [18328078] The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710. (FEMS Microbiol Lett. , 2008) comment Dynemicin生合成遺伝子クラスターの報告。 dynT5 Transporter ATP-ATPase subunit genbank: なし機能解析はされていない。
Related Reference	ACC P32010 NITE Adria_00130 PmId [1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991) [9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997) [15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005) [19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) [25721665] Characterization of a novel domain 'GATE' in the ABC protein DrrA and its role in drug efflux by the DrrAB complex. (Biochem Biophys Res Commun. , 2015) comment Streptomyces peucetius_drrA Daunorubicin/doxorubicin resistance ATP-binding protein drrA [PMID: 1924314] DrrAは排出ABC transporterのATP結合タンパク質。Mg2+依存的にATP (or GTP) を結合。 doxorubicin or daunorubicinの添加で活性が上昇。 DrrBはABC-2 type transporterといわれているもの。 -- さらにいくつかの続報がでており、DrrABはATP依存的なdoxorubicinとdaunorubicinの排出ポンプとされている(PMID: 15709779など)。上記著者らでの最新の続報は、[PMID: 19063901] -- 違うグループでも報告あり。上記既報をrefとして採用している。 [PMID: 20158521] drrAB(-)mutantはDNRに高い感受性あり。このmutantはwildと比べて、薬剤産生が10倍減。 key regulator dnrI and polyketide synthase gene dpsAの発現が激減。 regulatory genes dnrO and dnrNの発現は増。これらの結果から細胞内DNR濃度に基づくFeedback regulationを提唱。

Sequence

>putative drug resistance ABC transporter ATP-binding protein [DynT5]
MGLSKSFGKVVALNGLDVRLESGQVAAILGPNGAGKTTFLRMISTLTRPDGGTLRVCGID
VTAEPARVRQLIGLAGQHATVEPALTGRENLQMIARLFGQDRRTARASAEETLERLRLGE
AGDRLVRTYSGGMRRRLDLGASLVGRPQVLLLDEPTTGLDPQSRIELWDTIRALTTEGTD
VLLTTQYLEEADRLANTILIIDHGQVIASGTAKELKSQAGRDIVEIHVRDREDLDPAVRA
LTRIGAETPVVDHGSLMASVAVDSGPERLREALHEVSELGVHVEDVALRRPTLDEVFLSL
TGSTPAGEPRGAEPRSPESPAGIEPETVSRSSR

>putative drug resistance ABC transporter ATP-binding protein [DynT5]
GTGGGCCTCAGCAAGTCCTTCGGGAAGGTCGTCGCCCTCAACGGGCTCGACGTGCGCCTG
GAGTCCGGCCAGGTCGCCGCGATCCTCGGCCCCAACGGGGCCGGTAAGACCACCTTCCTG
CGCATGATCTCCACGCTCACCCGGCCGGACGGCGGCACGCTGCGGGTCTGCGGCATCGAC
GTGACCGCCGAGCCGGCCCGGGTCCGCCAGCTCATCGGCCTCGCCGGGCAGCACGCGACG
GTCGAGCCCGCGCTGACCGGGCGGGAGAACCTCCAGATGATCGCCCGGCTGTTCGGCCAG
GACCGGCGTACCGCCCGGGCCAGCGCCGAGGAGACGCTGGAGCGCCTGCGCCTCGGCGAG
GCCGGCGACCGGCTGGTCCGCACCTACTCCGGCGGCATGCGGCGCCGGCTCGACCTGGGC
GCCAGCCTGGTCGGCCGCCCGCAGGTGCTGCTGCTCGACGAGCCCACCACCGGGCTCGAC
CCGCAGAGCCGGATCGAGCTGTGGGACACCATCCGGGCGCTCACCACCGAGGGCACCGAC
GTGCTGCTCACCACGCAGTACCTGGAGGAGGCGGACCGGCTCGCCAACACCATCCTGATC
ATCGACCACGGGCAGGTGATCGCCTCCGGCACGGCCAAGGAGCTGAAGAGCCAGGCCGGC
CGCGACATCGTGGAGATCCACGTCCGCGACCGGGAGGATCTCGACCCGGCCGTGCGGGCG
CTGACCCGGATCGGCGCCGAGACGCCTGTCGTCGACCACGGCTCGCTGATGGCCTCGGTG
GCGGTCGACTCCGGTCCGGAGCGGCTCCGCGAGGCGTTGCACGAGGTCAGCGAGCTGGGC
GTGCACGTCGAGGACGTGGCCCTGCGCCGGCCCACGCTCGACGAGGTGTTCCTGTCGCTG
ACCGGGTCGACCCCGGCGGGCGAGCCCCGTGGCGCCGAGCCGCGGTCGCCCGAGTCCCCC
GCGGGCATCGAGCCCGAGACCGTGTCGAGGAGTTCCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003439 ABC transporter-like (Domain) PF00005 ABC_tran PS50893 ABC_TRANSPORTER_2 IPR003593 AAA+ ATPase domain (Domain) SM00382 AAA IPR005894 Daunorubicin resistance ABC transporter ATP-binding subunit (Family) TIGR01188 DrrA IPR017871 ABC transporter, conserved site (Conserved_site) PS00211 ABC_TRANSPORTER_1
SignalP	No significant hit
TMHMM	No significant hit

Dynm_00450 Dynm_00470