Salino3_00270 : CDS information

Location

Organism	Streptomyces albus
Strain	XM211
Entry name	Salinomycin
Contig	JN033543
Start / Stop / Direction	96,333 / 97,292 / + [in whole cluster] 96,333 / 97,292 / + [in contig]
Location	96333..97292 [in whole cluster] 96333..97292 [in contig]
Type	CDS
Length	960 bp (319 aa)

Annotation

Category	4.3 transport
Product	putative ABC transporter ATP-binding protein
Product (GenBank)	putative ABC transporter ATP-binding protein
Gene
Gene (GenBank)	slnTI
EC number
Keyword
Note
Note (GenBank)	SlnTI
Reference	ACC H6D583 PmId [22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012) comment polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いてStreptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。 SlnTI (319aa) : ABC transporter ATP-binding protein 配列解析のみ。 SlnTI と SlnTII はおそらく産生株S. albus XM211にsalinomycin抵抗性を与える。
Related Reference	ACC Q54406 PmId [8196549] ( , ) comment Blast 45th, id64%, 1e-102 Streptomyces longisporoflavus_TnrB2 TnrB2 protein Streptomyces longisporoflavusで、tetronasin-resistance determinantのスクリーニング。まず2つのdeterminantが見つかりtnrB (ptetR51)、tnrA (ptetR11)と名付けた。Streptomyces albusにクローニングしたところ、tnrB (ptetR51)の方だけがtetronasin抵抗性を示した。 tnrB determinantを更に解析したところ、ORF1, 2, 3が見つかった。ORF2(TnrB2)の産物はABC superfamilyのATP-binding domainsに著しい類似性があり、ORF3(TnrB3)はhydrophobic protein をコードしているだろうと思われた。 tnrBによるtetronasin resistanceのメカニズムはATP-dependent efflux systemだろう。
Related Reference	ACC H1ZZU7 NITE Salino_01050 PmId [22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012) comment 配列は[H6D583]と全く同じ。株違い。 monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。 salH (319aa) : putative salinomycin export 詳細コメントなし。
Family/Domain	FD IPR003439 comment [IPR003439] ABC transporter-like (Domain) Molecular Function; ATP binding, ATPase activity

Sequence

>putative ABC transporter ATP-binding protein [putative ABC transporter ATP-binding protein]
MAPNEYAITAEGLTKDFGKFRALDGLDLSVRTGEVHGFLGPNGAGKSTTIRALLGLLKPT
AGKATLLGKDPWADAVEVHHKLAYVPGDVSLWRNLSGGEAIDLFGTLRGGIDKSRKAELI
ERFQLDPTKKCRAYSKGNRQKVALVSAFASDTELLILDEPTSGLDPLMEAVFQECVKEVK
AQGRTVLLSSHILAEVEALCDRVSIVRRGRTVESGSLQELRHLTRTSLSVETQRPATGLA
ELAGVHNAVAEGNHLKLDVDNAQLEPVLAKVLGFGVVSLNSAPPTLEELFMRHYGDAPAE
DGDGGKDGADRAKAEAATR

>putative ABC transporter ATP-binding protein [putative ABC transporter ATP-binding protein]
TTGGCGCCCAACGAGTACGCAATCACCGCGGAAGGCCTCACCAAGGACTTCGGCAAGTTC
CGCGCGCTGGACGGTCTCGACCTGTCCGTGCGCACGGGAGAGGTCCACGGCTTCCTCGGC
CCCAACGGTGCGGGTAAATCCACCACCATCCGTGCCCTGCTCGGCCTGCTCAAGCCCACC
GCGGGCAAGGCCACCCTGCTCGGCAAGGACCCCTGGGCCGACGCCGTCGAGGTGCACCAC
AAGCTCGCCTACGTCCCCGGCGACGTCAGCCTGTGGCGCAACCTCAGCGGCGGCGAGGCC
ATCGACCTGTTCGGGACGCTGCGCGGCGGCATCGACAAGTCCCGCAAGGCGGAGCTGATC
GAGCGCTTCCAGCTCGACCCGACCAAGAAGTGCCGCGCCTACTCCAAGGGCAACCGGCAG
AAGGTCGCCCTGGTCTCCGCCTTCGCCAGCGACACCGAGCTGCTCATCCTCGACGAGCCC
ACCTCCGGACTCGACCCCCTGATGGAGGCCGTCTTCCAGGAGTGCGTCAAGGAGGTCAAG
GCGCAGGGCCGCACCGTCCTGCTCTCCAGCCACATCCTCGCCGAGGTCGAGGCGCTCTGC
GACCGCGTCAGCATCGTGCGCCGCGGCCGCACCGTGGAATCGGGCTCCCTGCAGGAGCTG
CGCCACCTCACCCGCACCTCGCTCAGCGTCGAGACGCAGCGCCCGGCCACCGGGCTCGCG
GAGCTGGCCGGCGTCCACAACGCGGTGGCCGAGGGCAACCACCTCAAACTCGACGTGGAC
AACGCCCAGTTGGAGCCCGTACTCGCCAAGGTCCTCGGCTTCGGCGTGGTCTCCCTGAAC
AGCGCCCCGCCCACCCTGGAGGAGCTGTTCATGCGCCACTACGGCGACGCCCCCGCCGAG
GACGGCGACGGCGGCAAGGACGGCGCGGACCGCGCGAAGGCGGAGGCGGCCACCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003439 ABC transporter-like (Domain) PF00005 ABC_tran PS50893 ABC_TRANSPORTER_2 IPR003593 AAA+ ATPase domain (Domain) SM00382 ATPases associated with a variety of cellula IPR017871 ABC transporter, conserved site (Conserved_site) PS00211 ABC_TRANSPORTER_1
SignalP	No significant hit
TMHMM	No significant hit

Salino3_00260 Salino3_00280