Salino3_00270 Salino3_00270   Salino3_00290 Salino3_00290

Salino3_00280 : CDS information

close this sectionLocation

Organism
StrainXM211
Entry nameSalinomycin
Contig
Start / Stop / Direction97,289 / 98,929 / + [in whole cluster]
97,289 / 98,929 / + [in contig]
Location97289..98929 [in whole cluster]
97289..98929 [in contig]
TypeCDS
Length1,641 bp (546 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter permease protein
Product (GenBank)putative antibiotic ABC transporter efflux pump
Gene
Gene (GenBank)slnTII
EC number
Keyword
Note
Note (GenBank)
  • SlnTII
Reference
ACC
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
comment
polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いてStreptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。

SlnTII (546aa) : ABC transporter efflux pump

配列解析のみ。
SlnTI と SlnTII はおそらく産生株S. albus XM211にsalinomycin抵抗性を与える。
Related Reference
ACC
Q54407
PmId
[8196549] ( , )
comment
Blast 100th, id39%, 2e-35, C末200aaほど足りない
Streptomyces longisporoflavus_TnrB3
TnrB3 protein

Streptomyces longisporoflavusで、tetronasin-resistance determinantのスクリーニング。
まず2つのdeterminantが見つかりtnrB (ptetR51)、tnrA (ptetR11)と名付けた。Streptomyces albusにクローニングしたところ、tnrB (ptetR51)の方だけがtetronasin抵抗性を示した。

tnrB determinantを更に解析したところ、ORF1, 2, 3が見つかった。ORF2(TnrB2)の産物はABC superfamilyのATP-binding domainsに著しい類似性があり、ORF3(TnrB3)はhydrophobic protein をコードしているだろうと思われた。

tnrBによるtetronasin resistanceのメカニズムはATP-dependent efflux systemだろう。
ACC
H1ZZU8
NITE
Salino_01060
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
comment
配列は[H6D584]と全く同じ。株違い。

monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。

salI (546aa) : putative salinomycin export

詳細コメントなし。

close this sectionSequence

selected fasta
>putative ABC transporter permease protein [putative antibiotic ABC transporter efflux pump]
MSEKSPYTGTQALLKLATRRDRIQLAVWMYGTAALAYSAANSVKSTYENEKARVDAVQLL
SDNPALLLTRGAPVGSSQGAVSMVQVFSYLCVLAALMSTMAVVRHTRQNEETGRAELIGA
QPVGRQAGLMAALLLALQANIGLFIFLGIVLPLAGLPIGGSLAAAAAVASVGLAFAGVAA
VTAQLAGTARGANSLAGAAIGAAFLIRGVGDALGDVTGNGTVVHSAWPSWLSPIGWGTLV
HPFGDERWWVLALPLAFALLTMGLALRLSAERDLGAGLLADRPGPAEAPRALLSPLGLAW
RLQRGALLGWLVGIAVLGATIGAIGKSAADALKDNADLTDTLGKIGGGGTDDPAGVFFAS
MMVMVGAVAAGFTVQALLRLRAEETSGRLEAVLATSVSRGRWVAGHVLVAVLGVVALLLV
SGLAAGLTYGAATSDLGQALSDLTGAALAQAPAALVLAGFVVVVFGAVPGLTSGAAWGGL
ALCLVVGQLGGLLKLPQAVRDLSPFTHLPALPAAEAEALPFIVLLAVAAVLAAAGAALFR
RRDLAP
selected fasta
>putative ABC transporter permease protein [putative antibiotic ABC transporter efflux pump]
ATGAGCGAGAAGTCCCCCTACACCGGTACGCAGGCGCTGCTGAAGCTCGCCACGCGCCGC
GACCGGATCCAGCTCGCGGTGTGGATGTACGGCACGGCCGCCCTCGCCTACAGCGCGGCC
AACAGCGTCAAGAGCACGTATGAGAACGAGAAGGCGCGTGTTGACGCCGTCCAGTTGCTT
TCGGACAACCCGGCGCTCCTGCTGACCCGCGGCGCGCCCGTCGGCAGCAGCCAGGGCGCT
GTCTCGATGGTGCAGGTCTTCTCCTATCTGTGCGTGCTCGCCGCCCTGATGAGCACGATG
GCCGTGGTGCGCCACACCCGGCAGAACGAGGAGACCGGCCGCGCCGAACTCATCGGCGCC
CAGCCGGTGGGCCGCCAGGCCGGTCTGATGGCCGCCCTGCTGCTCGCCCTCCAGGCCAAT
ATCGGCCTCTTCATCTTCCTCGGCATCGTGCTGCCGCTGGCCGGTCTGCCGATCGGCGGC
TCGCTCGCCGCGGCGGCCGCCGTCGCCAGTGTCGGCCTCGCCTTCGCCGGTGTCGCCGCC
GTCACCGCCCAGCTCGCCGGTACCGCGCGCGGCGCCAACTCCCTTGCCGGGGCGGCGATC
GGCGCGGCCTTCCTGATCCGCGGCGTCGGCGACGCGCTCGGCGACGTCACCGGGAACGGC
ACGGTCGTCCACAGCGCCTGGCCGTCCTGGCTCTCGCCGATCGGCTGGGGCACCCTGGTG
CACCCCTTCGGCGACGAGCGCTGGTGGGTGCTCGCCCTGCCGCTCGCCTTCGCGCTGCTC
ACCATGGGGCTCGCGCTGCGGCTTTCGGCCGAGCGCGACCTCGGTGCGGGCCTCCTCGCG
GACCGGCCGGGACCGGCCGAGGCGCCGCGGGCCCTGCTCAGCCCGCTCGGCCTCGCCTGG
CGGCTGCAGCGCGGCGCCCTGCTCGGCTGGCTCGTCGGCATCGCCGTCCTGGGCGCCACC
ATCGGCGCGATCGGCAAGTCGGCCGCCGACGCCCTCAAGGACAACGCCGACCTCACCGAC
ACCCTCGGCAAGATCGGCGGCGGGGGTACGGACGACCCCGCGGGCGTCTTCTTCGCCTCC
ATGATGGTGATGGTGGGCGCCGTCGCCGCCGGGTTCACCGTGCAGGCGCTCCTGAGACTG
CGGGCCGAGGAGACCTCGGGGCGCCTGGAGGCCGTGCTCGCCACCTCGGTGAGCCGGGGC
CGCTGGGTCGCCGGCCATGTCCTGGTCGCCGTGCTCGGCGTGGTGGCTTTGCTGCTCGTC
TCCGGACTCGCCGCCGGCCTCACCTACGGCGCCGCCACCTCGGATCTCGGGCAGGCCCTC
AGCGACCTGACCGGCGCCGCGCTCGCCCAGGCCCCCGCCGCGCTCGTCCTCGCCGGGTTC
GTGGTCGTCGTCTTCGGCGCGGTGCCGGGCCTCACCTCGGGCGCCGCCTGGGGCGGACTC
GCCCTGTGCCTGGTCGTCGGACAGCTCGGCGGACTGCTGAAGCTGCCGCAGGCGGTGCGC
GACCTCTCGCCGTTCACGCACCTGCCCGCGCTGCCCGCGGCCGAGGCCGAGGCGCTGCCC
TTCATCGTCCTGCTCGCGGTGGCCGCGGTGCTCGCGGCGGCCGGTGCCGCCCTCTTCCGC
AGGCGCGACCTCGCCCCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-36] 0.356 Signal
Bacteria, Gram-positive   
TMHMM
[81-103] Transmembrane (o-i) [129-151] Transmembrane (i-o) [156-178] Transmembrane (o-i) [191-210] Transmembrane (i-o) [248-270] Transmembrane (o-i) [306-325] Transmembrane (i-o) [356-378] Transmembrane (o-i) [409-431] Transmembrane (i-o) [446-468] Transmembrane (o-i) [475-497] Transmembrane (i-o) [517-539] Transmembrane (o-i)
Transmembrane 11   
Salino3_00270 Salino3_00270   Salino3_00290 Salino3_00290
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