Salino3_00310 Salino3_00310   Salino3_00330 Salino3_00330

Salino3_00320 : CDS information

close this sectionLocation

Organism
StrainXM211
Entry nameSalinomycin
Contig
Start / Stop / Direction102,168 / 104,888 / + [in whole cluster]
102,168 / 104,888 / + [in contig]
Location102168..104888 [in whole cluster]
102168..104888 [in contig]
TypeCDS
Length2,721 bp (906 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative LuxR family transcriptional regulator
Product (GenBank)putative transcriptional regulator
Gene
Gene (GenBank)slnR
EC number
Keyword
Note
Note (GenBank)
  • SlnR; LuxR family
Reference
ACC
PmId
[22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012)
comment
polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いてStreptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。

SlnR (906aa) : LuxR family transcriptional regulator

SlnRはlasalocid gene clusterにあるLas4に似ていて、transcriptional regulatorsのLuxR familyに属する。
遺伝子置換によるslnRの不活化でsalinomycin産生は廃止されたため、SlnRはsalinomycin生合成のpositive pathway-specific regulatorであることが明らかに示された。
Related Reference
ACC
Q9S0N8
NITE
Aver_00010
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[19148632] Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis. (Appl Microbiol Biotechnol. , 2009)
[20012992] The pathway-specific regulator AveR from Streptomyces avermitilis positively regulates avermectin production while it negatively affects oligomycin biosynthesis. (Mol Genet Genomics. , 2010)
comment
Blast 4th, id42%, 1e-144
Streptomyces avermitilis_aveR
LuxR-family transcriptional regulator
avermectinにはpositiveに、oligomycinにはnegativeに作用するregulator.

--
[PMID:10449723](1999)
avermectin生合成gene clusterの報告。
AveR: positive regulator

aveR mutantsの表現型、DNA segmentsを導入することによるその相補特性、helix-turn-helix motifを持つタンパクへの配列類似から、AveRがpotential regulatory functionを持つことが示される。

---
[PMID: 19148632](2009)abstract
aveR 不活化mutantは、avermectin中間体をいかなるavermectin派生体にも変換できない。よってAveRは、avermectinの生合成遺伝子と修飾遺伝子の両方の発現を調節するpositive regulatorであることが示唆された。
また、多量のaveRはavermectinの完全な損失をもたらすことから、aveRには最高許容限界濃度がある。

---
[PMID: 20012992](2010)abstract
aveR deletion mutantはavermectin産生を消失し、oligomycin産生が増える。この表現型はaveR geneのsingle copyによって相補された。

AveRのC末HTH domainの除去はavermectin生合成を消失。
ave clusterとolm clusterの両方のpredicted promoter regionsがAveRのtarget sitesであり、AveRのDNA-binding activityはHTH domainに依存的である。
aveの構造遺伝子の転写はAveRに依存的であるが、olmの構造遺伝子とputative pathway-specific regulatory genesの転写はaveR mutantsで増える。

avermectin PKS_AVES1をコードするaveA1の転写がAveRによって活性化される。
aveRの過剰発現でavermectin産生増加。
ACC
H1ZZV2
NITE
Salino_01100
PmId
[22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012)
comment
配列は[H6D588]と全く同じ。株違い。

monC gene-based hybridisation probeを使ってsalinomycin(sal)生合成gene clusterを同定。

salJ (906aa) : regulatory

詳細記載なし。

close this sectionSequence

selected fasta
>putative LuxR family transcriptional regulator [putative transcriptional regulator]
MTLYDRDKEFTRVEKALDSTFSGTAGIVLLEGAVGCGRTEFLKECADLAQERGARVLYGG
AEGEALPHGLLRRLLAPLAPRGGQQRSIPTHQEFAETLAEAAEDAPVVLCVDDFPHADGP
SLGYLLHLARVVRRQRILLILTGPLHERTDSPVLGTELLRHPGFVRIPLTHLSPAGTAAL
LAEHAGAPVAAPPAERLHALSGGNPLLLRALLAEQPLPQRESQEWPAPEAGGPFTKALLT
CLHRSGSGVRELARAVAVLGEHATPELAARLAGTTPSRARQGLTALESAGLTRDCRLSHP
AVAAAVLDDLPAAERTALHGRAAHILDEAGVRAASVARHLLAAGTAEGPRAVRVLHEAAR
QSLVTGDDSRHAVACLELARSGCADESTDWDLDLRLAAVTWRFDPAAAEAHLKGPLAALR
AARLGAAAAGHLARLLVTQGRIEEAAEALEYLARATGSGGGAQCTREDPLRGLFALPPTE
DKPAPAPAAGESGAEQPAEPVRTVSAELRSSASLWTHPGTQADEQAIDKLLEGTPLAQST
FDPMVQAVRTLVHTDHADRAVTWCRTLRPEGSTAPGWNAVFGYLQAEAQLRLGDLAGAEQ
QAEAAMEAVADRGGLFQFGPLATLVLAQTARGSYDAVARQLQTSVPEELFTSVYSLNFLR
ARGHYYLATHRHKAALGEFLDAGRLARRWGLDRPQQLPWRTDAAEALLALGEHRQAERFV
LEQLATPDARGPRLRGVTLRLRAATTPLRQRPKLLTRAVDELRRSGDRFELARALADLGR
SLQMMGEGTRAAMVTRRAWHLAAQCGAVPLAEQILPGQGGQDRKPNTPEKEPAEAGASDT
DQLSESEKRVVSLAAYGYTNREISAKLYITVSTVEQHLTRVYRKLNITRRQELPMDLHFE
TLEHAG
selected fasta
>putative LuxR family transcriptional regulator [putative transcriptional regulator]
GTGACTCTTTACGACCGTGACAAGGAATTCACACGCGTCGAAAAGGCGCTCGACTCAACT
TTTTCCGGGACGGCCGGAATCGTCCTTCTGGAGGGGGCGGTCGGCTGCGGCCGCACCGAG
TTCCTCAAGGAGTGCGCCGACCTCGCCCAGGAGCGCGGCGCCCGCGTCCTGTACGGCGGC
GCCGAGGGCGAGGCACTCCCGCACGGACTGCTGCGCAGGCTGCTCGCCCCGCTGGCCCCG
CGCGGCGGACAGCAGCGGAGCATCCCGACCCACCAGGAGTTCGCCGAGACCCTCGCCGAG
GCGGCCGAGGACGCACCGGTGGTCCTGTGCGTCGACGACTTCCCGCACGCCGACGGGCCC
TCCCTCGGCTACCTCCTCCACCTCGCCCGCGTGGTGCGCCGCCAGCGGATCCTGCTGATC
CTCACCGGCCCCCTGCACGAGCGCACCGACAGCCCCGTCCTCGGCACCGAACTGCTGCGC
CACCCCGGCTTCGTACGGATCCCGCTCACCCATCTGAGCCCCGCCGGAACCGCCGCGCTC
CTCGCCGAGCACGCCGGTGCCCCGGTGGCCGCGCCGCCCGCGGAGCGGCTGCACGCCCTC
AGCGGCGGCAACCCGCTGCTGCTGCGCGCCCTGCTCGCCGAACAGCCCCTGCCGCAGCGG
GAGTCGCAGGAGTGGCCCGCGCCCGAAGCCGGGGGCCCCTTCACCAAGGCGCTGCTCACC
TGCCTGCACCGCAGCGGCTCCGGAGTACGCGAACTGGCCCGCGCCGTCGCGGTCCTCGGT
GAGCACGCCACCCCGGAACTGGCGGCCCGGCTGGCCGGCACCACCCCCAGCCGCGCCCGG
CAGGGCCTGACCGCCCTGGAGAGCGCGGGCCTGACCCGCGACTGCCGCCTGAGCCACCCG
GCCGTGGCCGCCGCCGTCCTCGACGACCTGCCCGCGGCCGAACGCACCGCCCTGCACGGC
CGCGCCGCGCACATCCTCGACGAGGCCGGGGTACGGGCCGCTTCGGTCGCCCGGCACCTG
CTCGCCGCCGGGACCGCCGAGGGCCCCCGGGCGGTGCGCGTGCTCCACGAGGCCGCCCGG
CAGTCCCTGGTCACCGGCGACGACTCCCGGCACGCGGTGGCCTGCCTGGAACTGGCCCGC
AGCGGCTGCGCGGACGAGAGCACCGACTGGGACCTCGACCTGCGGCTCGCCGCCGTCACC
TGGCGCTTCGACCCGGCAGCCGCCGAGGCGCACCTGAAGGGCCCGCTCGCCGCGCTGCGC
GCCGCACGCCTCGGCGCGGCCGCCGCCGGGCACCTCGCCCGGCTCCTGGTCACCCAGGGG
CGGATCGAGGAGGCCGCCGAGGCCCTGGAATACCTGGCCCGCGCCACCGGTTCCGGCGGC
GGGGCCCAGTGCACCCGGGAGGACCCGCTGCGCGGACTCTTCGCGCTGCCGCCCACCGAG
GACAAGCCCGCGCCCGCCCCGGCCGCCGGGGAGAGCGGCGCCGAGCAGCCCGCCGAGCCG
GTGCGCACGGTCTCCGCCGAGCTGCGCTCCTCGGCCTCGCTGTGGACCCACCCGGGCACC
CAGGCCGACGAGCAGGCCATCGACAAGCTCCTCGAAGGCACCCCGCTGGCCCAGAGCACC
TTCGACCCGATGGTCCAGGCGGTCCGCACCCTGGTGCACACCGACCACGCCGACCGCGCG
GTGACCTGGTGCCGGACGCTGCGCCCCGAGGGCAGCACCGCCCCCGGCTGGAACGCCGTC
TTCGGCTACCTCCAGGCCGAGGCGCAGCTGCGCCTCGGCGACCTCGCGGGCGCGGAGCAG
CAGGCCGAGGCCGCGATGGAGGCGGTCGCCGACCGCGGCGGCCTCTTCCAGTTCGGTCCG
CTCGCCACCCTCGTGCTCGCGCAGACCGCGCGGGGCAGCTACGACGCGGTGGCCCGCCAG
CTCCAGACCTCCGTGCCCGAGGAGCTCTTCACCAGCGTCTACTCCCTCAACTTCCTGCGC
GCCCGCGGCCATTACTACCTGGCGACCCACCGCCACAAGGCCGCGCTCGGGGAGTTCCTC
GACGCGGGCAGGCTGGCCCGCCGCTGGGGCCTGGACCGGCCGCAGCAGCTGCCCTGGCGC
ACCGACGCCGCCGAGGCGCTGCTCGCCCTCGGGGAGCACCGGCAGGCCGAGCGCTTCGTC
CTCGAACAGCTCGCCACCCCGGACGCCCGCGGCCCCCGGCTGCGCGGGGTCACCCTGCGG
CTGAGGGCGGCGACCACCCCGCTGCGCCAGCGCCCCAAGCTGCTGACCCGCGCGGTCGAC
GAACTCCGCCGCTCCGGCGACCGCTTCGAACTCGCCCGCGCCCTCGCCGACTTGGGCCGC
TCGCTGCAGATGATGGGGGAGGGCACCCGGGCCGCCATGGTCACCCGGCGCGCCTGGCAC
CTGGCCGCGCAGTGCGGGGCCGTCCCGCTCGCCGAGCAGATACTCCCCGGCCAGGGCGGC
CAGGACCGCAAGCCGAACACCCCCGAGAAGGAGCCCGCCGAGGCCGGCGCGAGCGACACC
GACCAGCTCAGCGAGTCGGAGAAGCGGGTGGTCTCGCTGGCCGCCTACGGCTACACCAAC
CGGGAGATCTCGGCGAAGCTCTACATCACCGTGAGCACCGTCGAACAGCACCTCACCCGC
GTCTACCGCAAGCTCAACATCACCCGCCGCCAGGAACTGCCGATGGACCTGCACTTCGAG
ACCCTGGAGCACGCGGGTTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[841-893] 3.7e-11 PF00196
PF00196   GerE
[843-857] 4.2e-09 PR00038 [857-873] 4.2e-09 PR00038 [873-885] 4.2e-09 PR00038
PR00038   HTHLUXR
[857-884] PS00622
PS00622   HTH_LUXR_1
[836-902] 14.833 PS50043
PS50043   HTH_LUXR_2
[840-897] 1.3e-17 SM00421
SM00421   helix_turn_helix, Lux Regulon
IPR011990 Tetratricopeptide-like helical (Domain)
[760-851] 7.4e-13 G3DSA:1.25.40.10
G3DSA:1.25.40.10   no description
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[852-893] 1.7e-15 G3DSA:1.10.10.10
G3DSA:1.10.10.10   no description
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[821-893] 1.4e-13 SSF46894
SSF46894   C-terminal effector domain of the bipartite response regulators
SignalP No significant hit
TMHMM No significant hit
Salino3_00310 Salino3_00310   Salino3_00330 Salino3_00330
Page top