Salino3_00400 : CDS information

Location

Organism	Streptomyces albus
Strain	XM211
Entry name	Salinomycin
Contig	JN033543
Start / Stop / Direction	115,811 / 116,650 / + [in whole cluster] 115,811 / 116,650 / + [in contig]
Location	115811..116650 [in whole cluster] 115811..116650 [in contig]
Type	CDS
Length	840 bp (279 aa)

Annotation

Category	5.1 general function
Product	putative phosphopantetheinyl transferase
Product (GenBank)	putative 4'-phosphopantetheinyl transferase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	Orf19
Reference	ACC H6D596 PmId [22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012) comment polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いて、Streptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。 Orf19 (279aa) : 4'-Phosphopantetheinyl transferase 詳細記載なし。
Related Reference	ACC Q9RFL1 NITE Myxo_00010 PmId [10601310] New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1. (J Biol Chem. , 1999) [11134965] The mtaA gene of the myxothiazol biosynthetic gene cluster from Stigmatella aurantiaca DW4/3-1 encodes a phosphopantetheinyl transferase that activates polyketide synthases and polypeptide synthetases. (J Biochem. , 2001) comment Blast 87th, id29%, 4e-08 Stigmatella aurantiaca_mtaA MtaA [DoBISCUIT]phosphopantetheinyl transferase -- [PMID: 10601310](1999) Myxothiazol生合成gene clusterの同定論文。 MtaA: Ppant transferase Ppant=4'-phosphopantetheinyl 4'-phosphopantetheinyl transferase MtaAによってmyxothiazol合成は活性化される。 mtaAへのkanamycin resistance geneの挿入はmyxothiazol合成を阻害する。 MtaB-MtaGのACP, PCP domainは4'-phosphopantetheine cofactorのためのputative binding siteのProsite consensus signatureを含んでいる。 -- [PMID: 11134965](2001) MtaAをACP, PCPと異種性発現して、4'-phosphopantetheinyl transferases機能があることを確認。 MtaAは他種のACP, PCPも同様に加工でき、基質特異性がゆるい。
Family/Domain	FD IPR008278 comment [IPR008278] 4'-phosphopantetheinyl transferase (Domain) function: holo-[acyl-carrier-protein] synthase activity, magnesium ion binding

Sequence

>putative phosphopantetheinyl transferase [putative 4'-phosphopantetheinyl transferase]
MSTPAAPGLGRSLAVTPAEGGRDAIREALARHGTALVHGCLPDWETADQDNPAIRALRGR
DRERYLALPGAARARFAASRRLLAHLVAAALDTRPGELELGHDPTGRPRVRGLDEICLSL
SHTGRLLLAGLTTRGLLGVDAEAADRALRSRSLVRQLCTPWELGELALLPEAAGDAGLVR
LWTLKEAYTKAIGQGMQFPFTEFGFGFGDGPLRLLLPDGRPGADTAWSFGSFGLVSGGEA
YRVSAALLDEGRAPSPGLVLDAEVAGAVTAALYGGGPGP

>putative phosphopantetheinyl transferase [putative 4'-phosphopantetheinyl transferase]
GTGAGCACCCCGGCGGCCCCCGGTCTCGGCCGCTCCCTCGCCGTCACCCCGGCCGAGGGC
GGGCGGGACGCCATCCGCGAGGCCCTGGCGCGGCACGGCACCGCGCTGGTGCACGGCTGC
CTCCCCGACTGGGAGACCGCGGACCAGGACAACCCCGCCATCCGTGCGCTGCGGGGCCGG
GACCGGGAGCGCTACCTCGCCCTGCCCGGCGCGGCGCGCGCCCGCTTCGCCGCCTCGCGC
CGCCTGCTCGCCCATCTGGTGGCCGCCGCGCTGGACACCCGGCCCGGGGAGTTGGAGCTC
GGCCACGACCCCACCGGGCGGCCCCGCGTCCGCGGCCTCGACGAGATCTGCCTCAGCCTC
AGCCACACCGGGCGGCTCCTCCTCGCCGGGCTCACCACCCGGGGGCTCCTCGGCGTCGAC
GCCGAGGCCGCCGACCGGGCGCTGCGCTCCCGGAGCCTGGTCAGGCAGCTGTGCACGCCC
TGGGAGCTCGGTGAACTCGCCCTCCTCCCGGAGGCCGCGGGCGATGCCGGACTGGTCCGG
CTGTGGACCCTGAAGGAGGCGTACACCAAGGCGATCGGGCAGGGCATGCAGTTCCCCTTC
ACCGAGTTCGGCTTCGGCTTCGGGGACGGGCCGCTGCGGCTGCTGCTCCCGGACGGCCGC
CCCGGCGCGGACACCGCGTGGTCCTTCGGCAGCTTCGGCCTGGTGAGCGGCGGCGAGGCC
TACCGGGTGAGCGCCGCCCTGCTCGACGAGGGCCGCGCCCCTTCGCCGGGCCTGGTCCTC
GACGCGGAGGTGGCCGGGGCCGTCACCGCCGCGCTGTACGGCGGCGGCCCCGGGCCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR008278 4'-phosphopantetheinyl transferase (Domain) SSF56214 4-PPT_transf G3DSA:3.90.470.20 G3DSA:3.90.470.20 PF01648 ACPS
SignalP	No significant hit
TMHMM	No significant hit

Salino3_00390 Salino3_00410