Pier_00050 Pier_00050   Pier_00070 Pier_00070

Pier_00060 : CDS information

close this sectionLocation

Organism
Strain
Entry namePiericidin
Contig
Start / Stop / Direction30,858 / 36,479 / + [in whole cluster]
30,858 / 36,479 / + [in contig]
Location30858..36479 [in whole cluster]
30858..36479 [in contig]
TypeCDS
Length5,622 bp (1,873 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)PieA5
GenepieA5
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • PKS
Reference
ACC
PmId
[22365607] Elucidation of Piericidin A1 biosynthetic locus revealed a thioesterase-dependent mechanism of alpha-pyridone ring formation. (Chem Biol. , 2012)
comment
Piericidin A1生合成gene clusterの報告。

PieA5: Modular PKS (KS-ATa-DH-KR-ACP)
ATa = malonyl-CoAに特異的なAT

module 6 (KS, AT, DH, KR, ACP)

17kb fragment(pieA4-B2)を置換したmutantは、Piericidin A1の産生を完全に廃止した。

close this sectionPKS/NRPS Module

6 malonyl-CoA
KS28..403
AT573..893
DH943..1104
KR1434..1614
ACP1716..1786

close this sectionSequence

selected fasta
>polyketide synthase [PieA5]
MLEALRVSLKETRRLQQQNHQLTEARHEPIAIVGMACRYPGDVHSPEDLWRLVADGRDAL
SDFPAERGWDTEELYDPDPDRPGRSYVRQGCFVRDADRFDAAFFGISPREALAMDPQQRL
LLETSWEAFERAGIDPSSVQGSRTGVFVGAAPLGYAADPRSAEGSEGYLLTGTTVSVASG
RLAYALGLEGPALTVDTACSSSLVALHLASRALRDGECTMALVGGAAVMAAPHMFIEFSR
QRGLAPDGRCKAFAGAADGTAWGEGVGVLLVERLSDARRLGHPVLALVRGSAINQDGASN
GLTAPSGPAQQRVIRAALADARLTAGDVDAVEAHGTGTRLGDPIEAQALLATYGQDRPAE
RPLYLGSVKSNIGHTQLAAGVAGVIKTVLAMRHGVLPATLHVDEPTPQVDWSRGAVELLT
EQRAWPATDRPRRAGVSSFGISGTNAHVILEQPADEPVEEPAEEPAGEPVGQRPTVVPGE
APAGTPWLLSARSPEALRAQAGRLAAAVSGEPGWDRADVAWSLVTARADLDHRAVVLGAD
RTELLAGLDALHRAGSAPQLVRGPGPRGGKLAFLFTGQGAQRAGAGRELHRRFPVFAAEL
DKVCALLTERTGLPVREVLLAEEGSPEAARLDETAFTQAALFALEVALFRLAESWGLRPD
FVLGHSIGELAAAHVAGVFSLEDAVTAVAARGRLMQQLPAGGAMVAVEATEAEVAGLLAG
HEARASIAAVNGPRSVVVSGEEEAVTAVAERLRAEGRRTKRLTVSHAFHSPRMAGMLDPF
REVLAGLTLHEPTIPVVSNLTGRPVTEEIRTAEYWVRHVREAVRFHDGVRHLLDRGVTAL
LELGPDGVLSAMTQQAAGDRDDVAAVPLLRRGRPETDTVLAAVAQAHVRGVRVDWPACLT
DLAGPAGRRVDLPTYPFQGERYWPAAAGPAAEASELGVTAAGHPLLGASVTPAEGGGLIL
TGRLSVRSHRWLADHVILGSVVVPGTAYLELALHAAHLAGCTAVEELDQEAPLILTEAGA
VQVQLVVGAADESGRRSIGVYSRPDDGEAWTRHAGGTLAAEDGAIGWDHAGVWPPEGAVP
VDVADFYPSVARDGFVYGPSFRGLRAVWRAGEEVYGEVALPEPERAQAGGYGVHPALLDA
ALHAGLVGNTGEQVLLPFAWNGVRLHRPGAAVLRLRMAPAGPDAMSLQVADESGAPVASV
GALRARPVSVEQLRAAARAGLGDALFRVEWTPAPAAETVRGRWAVLGSPDDGTLGAGLDH
HPDVAALGAAVDAGLPQPDAVFLPVPDLPGADLPGADLPGADLPEAGADVPAAVRAAVHR
VGSALAAWAADDRLATSRLVLVTRRAVAAEHGEDVPQLTHAPLWGLVRAAQAEHPDRFGL
LDLDDASDPAAAFARAAAGAEPQLALRAGTVLTPRLVRARPAAPAPAPALDPDGTVLITG
GTGALGALFARHLVTAHGVRHLLLLGRRGPEAAGAAELTAELASLGAQVTVTACDVADRA
ALAAVLAAVPAEHPLTAVVHTAGVVADGALASLTEELFDAALRPKADAAWHLHELTREAG
LAAFVLFSSATATLGGAGQGGYTAANAFLDALAQHRTAQGLPARSLAWGLWEQRDGMAGA
LDDATLGRMRRSGVLPLTPEDGLALFDAALTGTDPTALPVRLDLAALRQTGPELPPLLRS
LAGTAAGPARRADAEAADGLKRRLAGLSEADRERALLDLVRTQVAVVLGHSDAASIDADR
AFGDLGFDSLTAVELRNRLTSATGLRLPATLVFDHPTAAALAGYLDAELPREGGHSTAPV
LAELDRLEAVLAAVAADHPDRARIAGRLRGVLSRWDDRRDPVASGESPAAGLELDSAEDD
EVFDFITNELGIS
selected fasta
>polyketide synthase [PieA5]
GTGCTGGAAGCGCTCCGGGTCTCGCTCAAGGAGACCCGGCGGCTGCAGCAGCAGAACCAC
CAGCTGACGGAGGCCCGGCACGAGCCGATCGCGATCGTCGGCATGGCCTGCCGCTACCCC
GGCGACGTCCACAGCCCCGAGGACCTGTGGCGGCTGGTCGCCGACGGCCGCGACGCCCTC
TCCGACTTCCCGGCCGAACGCGGCTGGGACACCGAGGAGTTGTACGACCCGGACCCGGAC
CGCCCCGGCCGGAGCTACGTCCGGCAGGGCTGCTTCGTCCGGGACGCGGACCGGTTCGAC
GCGGCGTTCTTCGGGATCAGCCCGCGCGAGGCCCTGGCGATGGACCCGCAGCAGCGGCTG
CTGCTGGAGACCTCCTGGGAGGCCTTCGAGCGCGCCGGTATCGACCCCTCCTCCGTCCAG
GGCAGCCGGACCGGGGTGTTCGTCGGCGCGGCCCCGCTCGGCTACGCTGCCGATCCGCGC
AGCGCCGAGGGCTCCGAGGGCTACCTGCTCACCGGTACCACCGTCAGCGTCGCCTCCGGC
CGGCTGGCGTACGCCCTCGGCCTGGAGGGCCCGGCGCTCACGGTGGACACCGCCTGCTCC
TCGTCCCTGGTCGCGCTGCACCTGGCGAGCCGGGCGCTGCGCGACGGCGAGTGCACGATG
GCGCTGGTCGGCGGCGCGGCCGTGATGGCGGCGCCGCACATGTTCATCGAGTTCAGCCGC
CAGCGCGGCCTCGCCCCCGACGGCCGCTGCAAGGCCTTCGCCGGCGCGGCCGACGGCACC
GCCTGGGGCGAGGGCGTCGGCGTCCTGCTGGTCGAGCGGCTCTCCGACGCCCGCCGCCTC
GGCCACCCGGTGCTCGCCCTGGTCCGCGGCTCGGCGATCAACCAGGACGGCGCCTCCAAC
GGCCTGACCGCCCCGAGCGGGCCCGCCCAGCAGCGGGTGATCCGGGCGGCGCTGGCCGAC
GCCCGGCTGACCGCCGGGGACGTGGACGCCGTCGAGGCGCACGGAACGGGCACCAGGCTC
GGCGACCCGATCGAGGCCCAGGCGCTGCTCGCCACCTACGGCCAGGACCGGCCGGCCGAA
CGGCCGCTCTACCTCGGCTCGGTGAAGTCCAACATCGGCCACACCCAGCTCGCCGCGGGC
GTCGCCGGAGTCATCAAGACGGTGCTGGCGATGCGGCACGGCGTGCTGCCCGCCACCCTG
CACGTGGACGAGCCGACGCCGCAGGTCGACTGGTCACGCGGCGCGGTCGAGCTGCTCACC
GAGCAGCGCGCCTGGCCGGCCACCGACCGGCCGCGGCGCGCGGGCGTCTCCTCGTTCGGG
ATCAGCGGCACCAACGCCCACGTCATCCTGGAGCAGCCGGCCGACGAGCCCGTCGAGGAG
CCGGCCGAGGAGCCGGCCGGCGAGCCCGTCGGGCAGCGCCCGACCGTCGTGCCCGGTGAG
GCGCCCGCCGGCACCCCGTGGCTGCTCTCCGCCCGCAGCCCGGAGGCGCTGCGCGCCCAG
GCCGGCCGGCTGGCCGCCGCCGTCTCGGGCGAGCCCGGGTGGGACCGGGCGGACGTCGCC
TGGTCGTTGGTCACCGCCCGGGCCGATCTCGACCACCGCGCGGTGGTGCTCGGCGCCGAC
CGCACCGAGCTGCTCGCCGGTCTCGACGCGCTGCACCGGGCCGGAAGCGCTCCGCAGCTG
GTCCGGGGTCCGGGTCCGCGCGGCGGGAAGCTGGCGTTCCTGTTCACCGGTCAGGGCGCG
CAGCGGGCCGGCGCCGGCCGCGAACTGCACCGCCGCTTCCCGGTGTTCGCCGCCGAGCTG
GACAAGGTCTGCGCCCTGCTGACGGAGCGGACCGGGCTGCCGGTGCGCGAGGTGCTGCTC
GCCGAGGAGGGTTCGCCCGAGGCGGCGCGGCTGGACGAGACCGCCTTCACCCAGGCCGCC
CTGTTCGCGCTGGAGGTGGCGCTGTTCCGGCTGGCCGAATCCTGGGGCCTGCGGCCGGAC
TTCGTGCTCGGGCACTCGATCGGCGAGCTGGCGGCGGCGCACGTCGCCGGGGTGTTCTCG
CTGGAGGACGCGGTGACGGCGGTCGCCGCGCGCGGCCGGCTGATGCAGCAACTCCCCGCC
GGCGGCGCGATGGTGGCCGTCGAGGCGACGGAGGCGGAGGTGGCCGGGCTGCTCGCCGGC
CACGAGGCGCGGGCGAGCATCGCGGCCGTCAACGGCCCCCGCTCGGTGGTGGTCTCCGGC
GAGGAGGAGGCGGTCACCGCGGTCGCCGAACGGCTCCGCGCCGAGGGCCGCCGCACCAAG
CGGCTGACCGTCAGCCACGCCTTCCACTCCCCGCGGATGGCGGGCATGCTCGACCCGTTC
CGCGAGGTGCTCGCCGGGCTGACCCTGCACGAGCCGACGATCCCCGTCGTCTCCAACCTC
ACCGGCCGGCCGGTCACCGAGGAGATCCGCACCGCGGAGTACTGGGTCCGCCACGTGCGC
GAGGCCGTGCGCTTCCACGACGGCGTGCGGCACCTGCTCGACCGCGGGGTCACCGCCCTG
CTGGAGCTCGGCCCGGACGGCGTGCTCTCCGCCATGACCCAGCAGGCCGCCGGGGACCGG
GACGACGTCGCGGCCGTGCCGCTGCTGCGCCGGGGCCGCCCGGAGACCGACACCGTGCTG
GCCGCCGTCGCGCAGGCGCACGTGCGGGGCGTCCGGGTGGACTGGCCGGCCTGTCTCACC
GACCTGGCCGGCCCGGCCGGGCGCCGGGTGGACCTGCCGACGTACCCGTTCCAGGGCGAG
CGGTACTGGCCGGCCGCCGCCGGTCCGGCCGCCGAGGCGTCCGAGCTCGGCGTGACGGCG
GCCGGGCATCCGCTGCTCGGCGCCTCCGTCACCCCGGCGGAGGGCGGCGGGCTGATCCTC
ACCGGCCGGCTGTCGGTCCGCTCGCACCGCTGGCTCGCCGACCACGTCATCCTCGGCAGC
GTGGTGGTGCCCGGCACCGCCTACCTGGAGCTGGCCCTGCACGCGGCGCACCTGGCCGGC
TGCACGGCGGTGGAGGAGCTGGACCAGGAGGCGCCGCTGATCCTCACCGAGGCCGGCGCG
GTGCAGGTGCAGCTGGTGGTCGGTGCGGCCGACGAGTCGGGCCGGCGGTCGATCGGCGTG
TACTCGCGCCCGGACGACGGCGAGGCGTGGACCCGGCACGCCGGCGGCACGCTCGCCGCG
GAGGACGGGGCGATCGGCTGGGACCACGCGGGCGTCTGGCCGCCCGAGGGCGCGGTGCCG
GTGGACGTGGCCGACTTCTACCCGTCGGTCGCCCGGGACGGCTTCGTCTACGGCCCGTCG
TTCCGCGGCCTGCGGGCGGTCTGGCGCGCGGGCGAGGAGGTGTACGGCGAGGTCGCCTTG
CCGGAGCCGGAGCGGGCGCAGGCCGGCGGGTACGGCGTCCACCCGGCGCTGCTCGACGCC
GCCCTGCACGCGGGCCTGGTCGGGAACACCGGTGAGCAGGTGCTGCTGCCGTTCGCGTGG
AACGGGGTGCGGCTGCACCGCCCGGGGGCGGCCGTGCTGCGGCTGCGGATGGCCCCGGCC
GGGCCGGACGCGATGTCCCTGCAGGTGGCCGACGAGTCCGGCGCGCCGGTGGCCTCCGTC
GGCGCGCTGCGGGCCCGGCCGGTCTCGGTCGAGCAGCTGCGCGCGGCGGCCCGGGCCGGG
CTCGGCGACGCCCTGTTCCGCGTCGAGTGGACGCCGGCCCCCGCGGCGGAGACCGTCCGC
GGGCGGTGGGCCGTCCTCGGCTCCCCGGACGACGGAACCCTGGGCGCGGGCCTCGACCAC
CACCCGGACGTCGCGGCACTCGGCGCAGCCGTCGACGCCGGGCTGCCGCAGCCGGACGCG
GTCTTCCTGCCCGTACCGGACCTGCCCGGAGCCGACCTGCCCGGAGCCGACCTGCCCGGG
GCCGACCTGCCGGAGGCAGGCGCAGACGTCCCCGCGGCGGTCCGTGCGGCCGTGCACCGG
GTGGGCTCCGCGCTGGCGGCGTGGGCGGCGGACGACCGCCTCGCCACCTCCCGGCTGGTC
CTGGTGACCCGCCGTGCAGTGGCCGCCGAGCACGGCGAGGACGTCCCGCAGCTCACGCAC
GCACCGCTGTGGGGGCTGGTCCGCGCCGCCCAGGCCGAGCACCCGGACCGCTTCGGCCTG
CTCGACCTCGACGACGCCTCGGACCCCGCCGCGGCCTTCGCCCGGGCCGCCGCCGGCGCC
GAGCCGCAGCTCGCCCTGCGGGCGGGCACCGTCCTGACGCCGCGTCTGGTCCGGGCCCGC
CCGGCGGCGCCCGCGCCGGCCCCCGCACTCGACCCCGACGGCACGGTGCTGATCACCGGC
GGCACCGGAGCGCTCGGCGCGCTGTTCGCCCGCCACCTGGTCACCGCCCACGGCGTGCGG
CACCTGCTGCTGCTCGGTCGCCGCGGCCCGGAGGCCGCGGGCGCCGCCGAGCTGACCGCC
GAACTCGCTTCCCTCGGAGCACAGGTGACGGTCACGGCCTGCGACGTGGCGGACCGGGCG
GCGCTCGCCGCCGTGCTCGCCGCCGTCCCCGCCGAGCACCCGCTCACCGCCGTCGTCCAC
ACGGCCGGCGTCGTCGCGGACGGCGCCCTGGCCTCGCTCACCGAGGAGCTGTTCGACGCC
GCGCTCCGTCCGAAGGCGGACGCCGCCTGGCACCTGCACGAGCTCACCCGCGAGGCCGGG
CTCGCCGCCTTCGTGCTGTTCTCGTCTGCGACGGCCACCCTCGGCGGCGCCGGCCAGGGC
GGCTACACGGCGGCCAACGCCTTCCTCGACGCCCTGGCCCAGCACCGCACCGCGCAGGGC
CTGCCCGCCCGGTCGCTCGCCTGGGGTCTCTGGGAGCAGCGCGACGGCATGGCGGGCGCC
CTGGACGACGCCACGCTCGGCCGGATGCGGCGCTCGGGCGTCCTGCCGCTCACCCCGGAG
GACGGCCTCGCCCTCTTCGACGCGGCGCTCACCGGGACCGACCCGACGGCGCTGCCGGTC
CGGCTGGACCTCGCCGCGCTGCGCCAGACCGGGCCCGAGCTCCCGCCGCTGCTGCGCAGC
CTGGCCGGGACGGCCGCCGGGCCCGCCCGCCGGGCCGACGCGGAGGCGGCGGACGGGCTG
AAGCGACGGCTCGCCGGCCTGTCCGAGGCCGACCGCGAACGCGCGCTGCTGGACCTGGTC
CGCACCCAGGTGGCGGTCGTGCTCGGCCACTCGGACGCCGCCTCGATCGACGCCGACCGC
GCCTTCGGCGACCTGGGCTTCGACTCGCTCACCGCGGTCGAGCTGCGCAACCGCCTGACC
TCCGCCACCGGCCTGCGGCTGCCGGCGACGCTGGTCTTCGACCACCCCACGGCGGCGGCG
CTGGCCGGCTACCTGGACGCCGAACTCCCGCGCGAGGGAGGGCATTCGACCGCCCCGGTG
CTCGCCGAACTGGACCGCCTGGAAGCCGTCCTCGCCGCGGTCGCCGCCGACCACCCCGAC
CGGGCGCGGATCGCAGGCCGGCTGCGGGGCGTCCTCTCCCGGTGGGACGACCGGCGCGAC
CCGGTGGCCTCCGGTGAATCCCCGGCGGCCGGACTGGAGTTGGACTCGGCCGAGGACGAC
GAGGTCTTCGACTTCATCACCAACGAACTCGGCATCTCCTGA
[6] KS28..403
[6] AT573..893
[6] malonyl-CoA766..770
[6] DH943..1104
[6] KR1434..1614
[6] ACP1716..1786
[6] KS82..1209
[6] AT1717..2679
[6] malonyl-CoA2296..2310
[6] DH2827..3312
[6] KR4300..4842
[6] ACP5146..5358

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[566-698] 4.49999999999996e-81 G3DSA:3.40.366.10 [766-882] 4.49999999999996e-81 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1744-1759] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1716-1786] PS50075
PS50075   ACP_DOMAIN
[1707-1793] 3.30000526807186e-24 SSF47336
SSF47336   ACP_like
[1714-1789] 6.1e-25 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1719-1785] 6.3e-13 PF00550
PF00550   PP-binding
IPR013968 Polyketide synthase, KR (Domain)
[1434-1613] 6.40000000000004e-61 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[28-277] 1.20000000000001e-96 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[285-403] 2.8e-48 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[573-893] 3.10000000000005e-72 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[570-864] 4.80002938964645e-73 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[700-765] 1.10000150671642e-18 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[28-287] 1.39999999999997e-87 G3DSA:3.40.47.10 [289-454] 4.09999999999995e-67 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[20-401] 2.59998233910302e-105 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1434-1619] 2.29999999999998e-35 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[190-206] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[574-873] 1.09999184471405e-127 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1717-1789] 2.9000035467798e-37 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[943-1104] 1.3000049540733e-78 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[30-455] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1434-1614] 2.99998750445706e-55 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Pier_00050 Pier_00050   Pier_00070 Pier_00070
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