Lasal2_00080 Lasal2_00080   Lasal2_00100 Lasal2_00100

Lasal2_00090 : CDS information

close this sectionLocation

Organism
StrainATCC 31180
Entry nameLasalocid
Contig
Start / Stop / Direction9,094 / 9,519 / + [in whole cluster]
9,094 / 9,519 / + [in contig]
Location9094..9519 [in whole cluster]
9094..9519 [in contig]
TypeCDS
Length426 bp (141 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative acyltransferase(fragment)
Product (GenBank)
Gene
Gene (GenBank)
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in lasalocid biosynthesis.
Note (GenBank)
  • ORF09; 3-oxoacyl-(acyl-carrier-protein) synthase III, fragment
Reference
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
comment
S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。

orf9: gene fragment
homolog: putative 3-oxoacyl-[acyl-carrier-protein]_Streptomyces sp. KO-3988

このORFとorf7,orf8はpseudogenesをエンコードすると推測された。

---
このclusterの配列は、GenBankではNRRL3382Rと登録されている。
orf9にはUniProt entryの割り当てがない。
Related Reference
ACC
B6ZK56
NITE
Lasal_00050
PmId
[19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009)
comment
GenBank,Uniprotでは、ATCC31180と登録されている。

S. lasaliensis ATCC35851におけるLasalocid生合成gene clusterの報告。

lsd3: Ethylmalonyl-CoA biosynthesis
homolog: putative 3-oxoacyl-[acyl-carrier-protein]_Streptomyces sp. KO-3988

詳細な機能はわかっていないと明記されている。

---
lsd3は全長342 a.a.
orf9は全長141 a.a.

orf9は、lsd3のC末端側と100%一致。この領域は[IPR013747] 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (Domain)がヒットする。
ACC
P68795
PmId
[11959561] Purification, characterization, and identification of novel inhibitors of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus. (Antimicrob Agents Chemother. , 2002)
comment
Staphylococcus aureus_fabH
3-oxoacyl-[acyl-carrier-protein] synthase 3
全長313a.a.

fabHの遺伝子をクローニングしてE. coliでN末にHis-tagを付けて過剰発現させて精製し、諸性質を調べている。FabHはhomodimeric structureで、malonyl-ACPに対してのKm値(1.76±0.40μM)と共に、acetyl-CoA, butyryl-CoA, isobutyryl-CoAに対して活性を持つことが示されている。

close this sectionSequence

selected fasta
>putative acyltransferase(fragment)
VPAGGSRRPASQETLASGEHYFHMQGRAVRDFVTTELPAGVRRLLTVCDIAPDAVDHFVP
HQANGMMLREVLPLLGLPRARTHLTVAEHANTGAASVPLALDTVRRGGLLEDGDTVLLAG
FGGGMSLGTALVRWDGAPGDG
selected fasta
>putative acyltransferase(fragment)
GTTCCCGCGGGGGGCAGCCGCAGGCCGGCGTCGCAGGAGACACTCGCCTCGGGCGAGCAC
TACTTCCACATGCAGGGGCGCGCCGTACGCGATTTCGTCACGACCGAACTCCCGGCAGGC
GTACGTCGGTTGCTCACCGTCTGCGACATCGCCCCGGACGCGGTGGACCACTTCGTCCCG
CACCAGGCCAACGGCATGATGCTGCGGGAGGTCCTGCCCCTGCTGGGACTGCCGCGGGCA
CGCACCCACCTCACGGTGGCCGAGCATGCCAACACTGGTGCGGCGTCCGTGCCGTTGGCG
CTGGACACGGTCCGGCGCGGGGGCCTCTTGGAGGACGGGGACACGGTGCTGCTCGCCGGG
TTCGGCGGAGGAATGTCCCTCGGCACCGCGCTGGTGCGCTGGGACGGGGCGCCCGGGGAC
GGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013747 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (Domain)
[49-134] 5.6e-28 PF08541
PF08541   ACP_syn_III_C
IPR016038 Thiolase-like, subgroup (Domain)
[5-134] 1.3e-32 G3DSA:3.40.47.10
G3DSA:3.40.47.10   no description
IPR016039 Thiolase-like (Domain)
[2-134] 1e-34 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit
Lasal2_00080 Lasal2_00080   Lasal2_00100 Lasal2_00100
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