Lasal2_00200 Lasal2_00200   Lasal2_00220 Lasal2_00220

Lasal2_00210 : CDS information

close this sectionLocation

Organism
StrainATCC 31180
Entry nameLasalocid
Contig
Start / Stop / Direction70,541 / 76,174 / + [in whole cluster]
70,541 / 76,174 / + [in contig]
Location70541..76174 [in whole cluster]
70541..76174 [in contig]
TypeCDS
Length5,634 bp (1,877 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)lasalocid modular polyketide synthase
Gene
Gene (GenBank)lasAVI
EC number
Keyword
Note
Note (GenBank)
  • polyketide synthase modules and related proteins
Reference
ACC
PmId
[19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008)
[22025396] Insights into lasalocid A ring formation by chemical chain termination in vivo. (Angew Chem Int Ed Engl. , 2011)
comment
GenBank,Uniprotでは、NRRL3382Rと登録されている。

---
[PMID:19025863](2008)
S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。

lasAVI: polyketide synthase KS, AT, DH, KR, ACP

module10(KS, AT, DH, KR, ACP)

Fig.1, scheme2で示したmodule10によって起こる二重結合はZ-olefinで示したが、このPKSのDH-KR domainはE-olefinを産生すると予測されていると述べている。

---
[PMID: 22025396](2011)
carba(dethia) N-acetyl cysteamine esters 5a-c(実際に作用するのは加水分解されたactive biosynthetic probes 6a-c)を用いて、lasalocid A PKSから中間体をin vivoで放出させ、その誘導体を分離・解析している。

推定されたdienes 8a-cでの3-OH基保持から、LasVI（LasAVIの書き間違いと思われる)のPKS DHは芳香環の形成に関与しないと示唆される。これは遺伝子解析に基づくこれまでの提唱と一致する。
Related Reference
ACC
B6ZK69
NITE
Lasal_00180
PmId
[19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009)
comment
GenBank,Uniprotでは、ATCC31180と登録されている。

S. lasaliensis ATCC35851におけるLasalocid生合成gene clusterの報告。

lsd16: polyketide biosynthesis, module 10

module10(KS, AT, dh, KR, ACP)

module10のDH-KR domainはE-olefinを提供し、C2-C7の環化はほとんど起こらないと推測。DH10はactive siteを保存しているがinactiveでundehydrated productを産生するだろうと推定している。

close this sectionPKS/NRPS Module

10 methylmalonyl-CoA
KS39..415
AT577..894
dh944..1108
KR1435..1615
ACP1720..1790

close this sectionSequence

selected fasta
>polyketide synthase [lasalocid modular polyketide synthase]
MREGALSNEERLRDYLKWVTTDLHDTRRRLREVQDAQSEPIAIVGMACRFPGGADSPDTF
WELIAEGRDAISGMPTDRGWDLDALYDDDPSHAGTSYAREGGFLPDAGHFDAALFGISPR
EALAMDPQQRLVLEASWEALERAGINPQSLRGSRTGVFVGHVASGYGAHVTDAPDGVEGY
LGTGTSASVASGRVAYTLGLEGPAVTVDTACSSSLVALHWAEQALRARECSLAIVAGVTV
LPSPLAFIEFSRQRGLAADGRCKPFSAAADGFGFAEGVGALLVQRLTDARREGREVLAVI
RGSAVNQDGASNGLTAPNGPSQQRVIRQALTNARLTPGDIDMVEAHGTGTALGDPIEAQA
LLATYGQDRPQDRPLRLGSVKSNIGHTQAAAGMAGLIKTVLAMRHGVMPRTLHLEEPTPH
VDWTSGRVSLLTEQTAWPASDRPRRAGVSSFGMSGTNAHVILEEAPQPSTADDADDAADG
PARQEDGSAPVPWVLSGKSPEALRAQAARLRAHVVASPAPVADLGLSLATTRAALEHRGV
ILATDLTDAVQGLDALASGEPAPGVVSGSAHPGSGVVFVFPGQGSQWTGMGRELLDSSPE
FAAYIAECDAALSDFVDWSLTDVLRGTEGAPGYDRVDVVQPALFAVMVSLARLWQHHGIH
PDAVIGHSQGEIAAAHIAGALTLNDAARIVALRSQALLPLAGLGGMTSLALPHDQALNLI
QPWGQDLSIASVNGPHSTVVSGTTHALDQLHTTCGTQGVRARRIPVDYASHSAQVESIRD
TVFQAATGINPQPTTIPLYSTVTGQPIDGTRLDADYWYTNLRHTVRFEETVRTLLSHGHR
HFIETTAHPVLTLALEETAQATTTDPTITGTLRRDHGDLTQLHTALATAWTHGLPVDWAS
LPAFRSARPVALPTYAFQRERYWLEEGHGGPADVEAAGLASPGHPLLGAMVRPAGGDPLV
LTGRLSLRTHPWLADHVALGTVLLPGAAFVELALRAGDEVGCECLEELTLETPLVLPERG
AVRTQVVIGEADGTGCRSLHVYSSPEDETDESGPWTRHASGVLAKQADVTPFEFAVWPPA
DAEPVDLDGFYEGLAEVGHGYGPVFRGLRAAWRLGPDVYAEVALPEEVGDEAGKFGLHPA
LLDASMHAIGVGDFFADTERVRLPFAWSDVSLHAVGARTLRVRLSPADTDAVALQLADAT
GRQVASVGSMVSRPVSPEQLPAARQSERDLFSQVWEPVRASGAAPDPDTDWVVLGADTGP
LVAAVSATGVRTAVHGDVVDLLAALDAGGAVPAVALLFCDPVATGDVAADARACTSAVLS
EVQTWLSDSRLESSQLVVVTRGAVTTGAEAEVTDLVQAPVWGLLRSAQSENPARFTVVDL
DDRPASHATLPGAVATALAVNEPQVALRDGVVVVPRLARTDGPAQPVVPDWDPSGTVLIT
GGTGTLGALFARHLVTRYGVRHLLLTSRRGAEAPGASELVAELAESGAAAEVVACDVADR
GALAGVLAGIDPDHPLRAVVHTAGVLDDGVIGTMTPERIDWVMRPKTDAAVHLHELTREQ
DLTAFVLFSSIAGLFGNPGQANYAAANVFTDALARHRHAQGLPALSLAWGLWADATGMTG
HLDEGERRRMSRSGVVPLSAEHGLALFDAACALDEPCVAPVQLDPPALRGLADAGVLPGV
LRGLVRATARRAADQGGGDGGAVLVRRLAGLAEADRHRELLDLVRTQAANVLGHASSASI
DPDHAFNELGFDSLTAVELRNLLNAATGLRLPPSLVFDYPTPTRLAEHLRAGLEPEIGAV
PHVDPEEAAVREALASLSLQQLREAGLVDVLLSLAGGDDAMAGTGSEAEEETLSIATMDV
DDLVQLALDTPETEFRK
selected fasta
>polyketide synthase [lasalocid modular polyketide synthase]
GTGCGCGAGGGTGCGCTCTCGAACGAGGAGAGGCTCCGCGACTACCTGAAGTGGGTCACC
ACCGACCTGCACGACACGCGCCGCCGGCTGCGCGAGGTCCAGGACGCCCAGAGCGAACCG
ATCGCGATCGTGGGGATGGCGTGCCGGTTCCCCGGCGGCGCCGACTCCCCGGATACCTTC
TGGGAGTTGATCGCCGAGGGCCGTGACGCGATATCGGGGATGCCCACCGATCGCGGCTGG
GACCTGGACGCCCTCTACGACGACGACCCCAGCCACGCCGGGACGTCGTACGCACGCGAG
GGCGGCTTCCTGCCCGACGCCGGTCACTTCGACGCGGCCCTCTTCGGGATCTCACCCCGT
GAGGCCCTCGCGATGGACCCGCAGCAGCGGCTCGTCCTCGAGGCATCCTGGGAGGCACTG
GAGCGGGCCGGTATCAACCCCCAGTCGCTGCGCGGCAGTCGTACCGGCGTCTTCGTCGGC
CATGTGGCCTCCGGATACGGCGCACACGTCACGGACGCTCCCGACGGGGTGGAGGGGTAC
CTGGGTACCGGCACCTCCGCCAGTGTCGCCTCGGGCCGCGTCGCCTACACCCTGGGGCTG
GAAGGCCCGGCGGTCACCGTCGACACCGCCTGTTCCTCCTCGCTGGTGGCGCTGCACTGG
GCCGAGCAGGCCCTGCGGGCCCGCGAGTGCTCGCTGGCGATCGTCGCCGGGGTGACGGTG
CTGCCGTCCCCGCTGGCGTTCATCGAGTTCAGCCGGCAGCGCGGACTCGCCGCCGACGGC
CGCTGCAAGCCCTTCTCGGCGGCGGCCGACGGCTTCGGTTTCGCGGAGGGCGTGGGCGCC
CTGCTCGTGCAACGCCTGACGGACGCGCGCCGCGAGGGCCGCGAGGTCCTGGCGGTGATC
CGCGGCTCGGCCGTGAACCAGGACGGCGCCAGCAACGGCCTCACCGCACCCAACGGCCCC
TCCCAGCAGCGGGTGATCCGCCAGGCCCTCACCAACGCCCGCCTCACGCCCGGCGACATC
GACATGGTGGAGGCCCACGGCACCGGCACCGCGCTCGGCGACCCGATCGAGGCGCAGGCG
TTGCTGGCGACGTACGGCCAGGACCGCCCGCAGGACCGCCCGCTGCGGCTGGGGTCCGTG
AAGTCCAACATCGGGCACACCCAGGCGGCCGCGGGCATGGCCGGGCTCATCAAGACCGTC
CTCGCGATGCGGCACGGGGTGATGCCCCGCACCCTGCACCTCGAGGAACCCACCCCTCAC
GTCGACTGGACCTCAGGCCGCGTCTCCCTGCTCACCGAGCAGACGGCCTGGCCCGCGTCG
GACCGTCCGCGCCGGGCCGGTGTCTCCTCCTTCGGCATGAGCGGCACCAACGCCCACGTG
ATTCTCGAGGAGGCCCCGCAGCCCTCCACCGCCGACGACGCCGACGACGCCGCTGACGGG
CCCGCTCGCCAGGAGGACGGGTCGGCGCCCGTCCCCTGGGTCCTGTCCGGCAAGAGTCCC
GAGGCCCTGCGTGCCCAGGCCGCACGCCTGCGTGCGCACGTGGTGGCGTCCCCCGCTCCG
GTCGCCGACCTCGGCCTGTCCCTGGCGACGACGCGCGCTGCGCTGGAACACCGTGGGGTG
ATCCTGGCGACGGACCTCACCGACGCCGTCCAGGGGCTGGACGCGCTGGCGTCAGGTGAG
CCCGCGCCGGGAGTCGTGTCCGGCTCCGCACATCCGGGGTCCGGGGTGGTGTTCGTGTTC
CCGGGTCAGGGTTCGCAGTGGACCGGCATGGGACGCGAACTCCTCGACAGCTCGCCCGAG
TTCGCCGCGTACATTGCCGAGTGCGATGCCGCGCTCAGCGACTTCGTGGACTGGTCGCTC
ACCGACGTCCTGCGCGGCACCGAAGGAGCCCCCGGCTACGACCGCGTCGACGTCGTCCAG
CCCGCCCTCTTCGCCGTCATGGTCAGCCTCGCCCGCCTCTGGCAACACCACGGCATCCAC
CCCGACGCCGTCATCGGCCACTCCCAAGGCGAAATCGCCGCCGCCCACATCGCCGGAGCC
CTCACCCTCAACGACGCCGCCCGCATCGTCGCCCTACGCTCCCAAGCCCTCCTCCCCCTC
GCCGGCCTCGGCGGCATGACCTCCCTCGCCCTCCCCCACGACCAAGCCCTCAACCTCATC
CAGCCCTGGGGCCAAGACCTGTCCATCGCCTCCGTCAACGGACCCCACTCCACCGTCGTC
TCCGGCACCACCCACGCCCTCGACCAACTCCACACCACCTGCGGCACCCAAGGAGTTCGG
GCCCGCCGCATCCCCGTCGACTACGCCTCCCACTCCGCCCAAGTCGAAAGCATCCGCGAC
ACCGTCTTCCAAGCAGCCACCGGCATCAACCCACAGCCGACCACCATCCCCCTCTACTCG
ACGGTCACCGGCCAACCCATCGACGGCACACGCCTCGACGCCGACTACTGGTACACCAAC
CTCCGCCACACCGTCCGCTTCGAGGAGACGGTCCGCACCCTCCTCAGCCACGGCCACCGC
CACTTCATCGAGACCACCGCCCACCCCGTCCTCACCCTCGCCCTCGAGGAGACGGCTCAG
GCCACCACCACCGACCCGACCATCACCGGCACCCTCCGCCGCGACCACGGCGACCTCACC
CAACTCCACACCGCCCTCGCCACCGCCTGGACCCACGGTCTCCCCGTCGACTGGGCGTCG
CTGCCCGCGTTCCGATCCGCCCGGCCCGTGGCGCTGCCGACCTACGCGTTCCAGCGCGAA
CGGTACTGGCTGGAGGAGGGGCACGGCGGTCCCGCGGACGTCGAAGCGGCGGGACTCGCC
TCTCCCGGACATCCGCTGCTCGGTGCCATGGTGCGACCCGCCGGAGGTGACCCGTTGGTC
CTCACCGGGCGGCTGTCCCTGCGCACGCACCCCTGGCTGGCGGACCACGTGGCATTGGGC
ACCGTACTGCTGCCGGGTGCCGCTTTCGTGGAACTGGCGTTGCGGGCGGGCGACGAGGTC
GGGTGCGAGTGCCTGGAGGAACTCACCCTCGAGACGCCGCTGGTCCTGCCGGAGCGCGGC
GCGGTGCGCACGCAGGTGGTGATCGGCGAGGCCGACGGGACCGGGTGCCGGTCGCTGCAC
GTGTACTCCTCCCCCGAGGACGAGACCGATGAGTCAGGTCCGTGGACGCGGCACGCGAGC
GGTGTGCTGGCCAAGCAGGCCGACGTGACGCCGTTCGAGTTCGCGGTGTGGCCGCCCGCG
GACGCCGAACCGGTCGATCTGGACGGCTTCTACGAGGGTCTGGCCGAGGTGGGACACGGC
TACGGGCCGGTGTTCCGTGGGCTGCGCGCGGCCTGGCGGCTGGGGCCGGACGTCTACGCG
GAGGTGGCCCTGCCGGAAGAGGTCGGGGACGAGGCGGGGAAGTTCGGGCTGCATCCGGCG
CTGCTCGACGCCTCGATGCACGCCATCGGTGTAGGGGACTTCTTCGCCGACACCGAGCGG
GTGCGGCTGCCGTTCGCATGGAGCGACGTGTCCCTCCATGCCGTGGGCGCCCGGACCTTG
CGGGTGCGGCTGTCGCCGGCCGACACGGACGCGGTGGCACTGCAGTTGGCGGACGCGACA
GGCCGCCAGGTCGCGTCCGTCGGGTCCATGGTGTCGCGTCCTGTCTCGCCCGAGCAGCTG
CCCGCCGCCCGACAGTCGGAGCGGGACTTGTTCAGCCAGGTCTGGGAGCCGGTGCGGGCT
TCGGGTGCCGCGCCGGATCCGGACACGGACTGGGTCGTCCTCGGGGCGGACACGGGCCCC
TTGGTCGCGGCCGTCTCGGCGACGGGCGTACGGACGGCGGTCCACGGTGACGTCGTAGAC
CTGTTGGCGGCTCTGGACGCGGGTGGGGCCGTGCCGGCCGTGGCCTTGCTGTTCTGCGAT
CCGGTGGCCACCGGTGACGTGGCCGCGGACGCCCGTGCCTGCACGTCCGCGGTGTTGAGC
GAGGTGCAGACCTGGCTGTCCGACTCCCGGCTGGAGTCGTCGCAGTTGGTCGTGGTCACG
CGAGGCGCGGTCACCACTGGTGCGGAGGCCGAGGTCACCGACCTGGTGCAGGCTCCGGTG
TGGGGGCTGCTGCGGTCGGCGCAGTCCGAGAACCCCGCACGCTTCACGGTGGTCGACCTG
GACGACCGTCCTGCCTCTCACGCGACGCTGCCGGGCGCGGTGGCCACCGCGCTCGCCGTG
AACGAGCCGCAGGTCGCCCTGCGTGACGGGGTGGTGGTCGTGCCGCGGCTCGCCCGGACG
GACGGACCGGCGCAGCCTGTGGTCCCGGACTGGGACCCTTCCGGCACCGTGCTGATCACC
GGCGGTACGGGCACCCTCGGTGCCCTCTTCGCCCGGCATCTGGTAACGCGGTACGGCGTG
CGGCACCTGCTGCTGACCAGCCGTCGCGGCGCGGAGGCTCCCGGGGCGTCCGAACTGGTC
GCGGAGCTGGCCGAGTCGGGGGCGGCGGCCGAGGTGGTCGCGTGCGACGTGGCCGACCGT
GGGGCGCTGGCCGGCGTGCTGGCGGGCATCGACCCCGACCACCCGCTGCGGGCGGTCGTG
CACACGGCGGGTGTGCTGGACGACGGTGTGATCGGCACCATGACCCCGGAGCGGATCGAC
TGGGTCATGCGGCCCAAGACGGACGCCGCCGTCCACCTCCACGAACTGACGCGGGAGCAG
GACCTGACGGCGTTCGTGCTGTTCTCCTCGATCGCCGGGCTGTTCGGCAACCCCGGTCAG
GCCAACTACGCGGCAGCGAACGTGTTCACGGACGCGCTGGCCCGGCACCGCCACGCACAG
GGTCTGCCGGCGCTGTCCCTCGCCTGGGGTCTGTGGGCCGACGCGACCGGCATGACAGGG
CACCTCGACGAGGGCGAACGACGGCGGATGTCCCGAAGCGGTGTGGTGCCGCTGTCGGCC
GAGCACGGGCTGGCCCTCTTCGACGCGGCCTGCGCTCTGGACGAGCCCTGTGTGGCCCCG
GTCCAGCTCGACCCGCCGGCCCTGCGCGGTCTGGCCGATGCCGGGGTGCTGCCCGGCGTC
CTGCGCGGCCTGGTCCGCGCCACGGCGCGGCGGGCCGCGGACCAGGGCGGCGGTGACGGC
GGTGCGGTGCTGGTCCGGCGGCTCGCCGGTCTGGCCGAGGCGGACCGCCACCGCGAACTC
CTCGACCTCGTGCGCACGCAGGCGGCGAACGTCCTCGGCCACGCCTCTTCCGCCTCCATC
GACCCCGACCACGCCTTCAACGAGCTCGGCTTCGACTCGCTCACGGCCGTCGAGCTGCGC
AACCTGCTCAACGCCGCCACCGGGCTGCGGCTGCCGCCCAGCCTCGTCTTCGACTACCCG
ACCCCGACCCGCCTCGCCGAGCACCTGCGCGCCGGCCTGGAACCGGAGATCGGCGCCGTA
CCGCACGTCGACCCGGAGGAGGCCGCCGTGAGGGAGGCGCTGGCCTCGCTGTCCTTGCAG
CAGTTGCGCGAGGCCGGACTGGTCGACGTGCTGCTGAGCCTCGCCGGGGGCGACGACGCC
ATGGCCGGCACCGGGAGCGAGGCGGAGGAGGAGACGCTGTCGATCGCCACGATGGACGTC
GACGATCTCGTGCAGCTCGCGCTCGACACCCCCGAGACGGAGTTCCGCAAATGA
[10] KS39..415
[10] AT577..894
[10] methylmalonyl-CoA768..772
[10] dh944..1108
[10] KR1435..1615
[10] ACP1720..1790
[10] KS115..1245
[10] AT1729..2682
[10] methylmalonyl-CoA2302..2316
[10] dh2830..3324
[10] KR4303..4845
[10] ACP5158..5370

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[573-700] 5.6e-75 G3DSA:3.40.366.10 [768-885] 5.6e-75 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR006162 Phosphopantetheine attachment site (PTM)
[1748-1763] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1712-1807] 3.69999438558106e-23 SSF47336
SSF47336   ACP_like
[1720-1790] PS50075
PS50075   ACP_DOMAIN
[1718-1792] 1.1e-22 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1723-1789] 7.59999999999999e-14 PF00550
PF00550   PP-binding
IPR013968 Polyketide synthase, KR (Domain)
[1435-1614] 1.40000000000001e-64 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[39-289] 2.59999999999995e-95 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[297-415] 3.29999999999997e-50 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[577-894] 6.49999999999992e-103 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[1-39] 8.39999858592306e-07 SSF101173
SSF101173   Polyketide_synth_docking
[7-32] 2e-13 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[575-864] 5.60004908290974e-67 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[702-767] 5.29999657550189e-16 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[38-300] 1.10000000000001e-89 G3DSA:3.40.47.10 [301-468] 3.79999999999991e-68 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[31-413] 1.19999063421924e-103 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1435-1618] 4.5e-38 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[202-218] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[579-876] 7.49999605851445e-124 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1721-1793] 2.69998997787809e-36 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[944-1108] 2.2999842048857e-87 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[41-467] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1435-1615] 4.39999001237724e-64 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Lasal2_00200 Lasal2_00200   Lasal2_00220 Lasal2_00220
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