Lasal2_00220 : CDS information

Location

Organism	Streptomyces lasaliensis
Strain	ATCC 31180
Entry name	Lasalocid
Contig	FM173265
Start / Stop / Direction	76,171 / 80,106 / + [in whole cluster] 76,171 / 80,106 / + [in contig]
Location	76171..80106 [in whole cluster] 76171..80106 [in contig]
Type	CDS
Length	3,936 bp (1,311 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	lasalocid modular polyketide synthase
Gene
Gene (GenBank)	lasAVII
EC number
Keyword
Note
Note (GenBank)	polyketide synthase modules and related proteins
Reference	ACC B5M9L4 PmId [19025863] Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. (Chembiochem. , 2008) comment GenBank,Uniprotでは、NRRL3382Rと登録されている。 S. lasaliensis ATCC31180におけるLasalocid生合成gene clusterの報告。 lasAVII: polyketide synthase KS, AT, ACP, TE integral C-terminal thioesteraseはマクロライドPKSのようにchain releaseを直接触媒すると推測している。 module11(KS, AT, ACP, TE)
Related Reference	ACC B6ZK70 NITE Lasal_00190 PmId [19129623] Identification of a gene cluster of polyether antibiotic lasalocid from Streptomyces lasaliensis. (Biosci Biotechnol Biochem. , 2009) comment GenBank,Uniprotでは、ATCC31180と登録されている。 S. lasaliensis ATCC35851におけるLasalocid生合成gene clusterの報告。 lsd17: polyketide biosynthesis, module 11 TE domainが存在することから、ポリケタイド鎖が切断された後ポリエーテル環形成が進行すると予想している。 module11(KS, AT, ACP, TE)

PKS/NRPS Module

malonyl-CoA

KS	35..411

AT	566..868

ACP	937..1007

TE	1105..1307

Sequence

>polyketide synthase [lasalocid modular polyketide synthase]
MTTSPEKIVEALRASLSENERLRRQNQQLTTASREPVAIVGMACRFPGGVESPEQLWRLL
TEGRDAVADFPSDRGWDLDSLYDPDPDHPGTSYTSQGGFLYDAGQFDPLLFGISPREALA
MDPQQRLLLETSWEVFERAGIDPSSLGGTRTGVFAGAIASGYAIGLPEFPEGVQGYVGTG
TSASVLSGRISYAFGLEGPAVTVDTACSSSLVALHLACQALRNGECSLALAGGVTVMAVP
GLFTEFSRQRGLAPDGRCKAFAAAADGTGFSEGVGVLLVERLSDARRNGHQVLAVVRGSA
VNQDGASNGLTAPNGPSQQRVIRQALANARLTPADVDAVEAHGTGTTLGDPIEAQALLAT
YGQNRTEDQPLWLGSVKSNIGHTQAAAGVAGVIKMVMALRHEVLPQTLHVDAPSPHIDWA
SGHVSLLTEELKWPGTDRPRRAGVSSFGISGTNAHVILEAAPPETPPADDEPERAAGPVP
WVLSARTRKALQSQAARLLTFTEETPDVSVAAVSSALLGTRAALDHRAVVLASDRVEFSR
RLHALASGRPASGVTTGSVRSGRLAFMFTGQGSQRLGMGHELYDTEPAFAAALEEAFAAL
DPHLPQPLRDIIFGDDPELLQQTQYAQPALFAVETALYRLLTDTYGLRPDYLLGHSIGEL
TAAHAAGVLTLPDAARLVTARGHLMQTAPTGGTMIAIQATEDEITEHLTPGVAIAALNSP
SSTVISGDTAEAEAIADRFRALGRKTSRLKVSHAFHSPHMDPVLDDFRQIAATLDYHPAR
IPVISNTTGQPAENLTSPDYWTQHIRRPVRFTDSLHHLTQQGVTTFLELGPDATLAMLAD
AHATAVLRRNSSEPQALRTALAELWVHGVPVAWPHSTDRRVDLPTYAFDHQHYWLDAAPG
TSATSVRPSVPVEAADAPPTLADRFEGLGAGERTSMAEELVRETVAVVLGFADPEDVDVS
TPFIKLGIDSLTAVELRRRLGGYTGLEIPAVVVFEHPTTHDLAEYLIEAMSAPPGATPDG
ASGAPSGVRATAAVDSLSDLFVRACGRGEFAEAHRLALGLAEYRPTFAGELDGARSPDVV
PLRRGTASPAVVCFPSFVWKPDFQQYLSLARELTEDRDVAVSGLPGFRPGEPLPASAEAL
ARALAAVVRRVAGGRRCVLLGHSSGGYVAAAVAAQLEEAGEAPAGLVLVDTPWWGSAGGL
GSQEWLPVINEMLLARGAAAGAEDSDAGDAWVTARAKYFSLDFAIAPRDVRTLLLRPAEP
LAGSAADAGWRAEWRPGTTVVEVPGNHFSMLDSAHAQACARAVEGWLATLG

>polyketide synthase [lasalocid modular polyketide synthase]
ATGACCACATCGCCAGAGAAGATCGTCGAGGCGCTGCGTGCATCGCTGTCGGAGAACGAG
CGGCTGCGACGCCAGAACCAGCAGCTCACCACGGCGTCCCGGGAGCCGGTGGCCATCGTC
GGCATGGCCTGCCGGTTCCCCGGCGGGGTGGAGAGCCCCGAGCAGCTGTGGCGGCTGCTG
ACCGAGGGCCGGGACGCCGTCGCGGACTTCCCGAGCGACCGCGGCTGGGACCTGGACTCC
CTCTACGACCCGGATCCCGACCACCCGGGGACGTCGTACACGAGCCAGGGCGGATTCCTG
TACGACGCGGGGCAGTTCGACCCGCTGCTGTTCGGCATCTCGCCCCGCGAGGCCCTGGCG
ATGGACCCCCAGCAGCGGCTGCTGCTGGAGACCTCCTGGGAGGTGTTCGAACGCGCCGGC
ATCGACCCGAGTTCGCTCGGCGGGACCCGGACCGGTGTGTTCGCCGGCGCCATCGCCTCC
GGCTACGCGATCGGTCTGCCGGAGTTCCCCGAGGGTGTCCAGGGGTACGTCGGCACCGGC
ACGTCGGCGAGCGTGCTGTCCGGGCGGATCTCCTACGCCTTCGGTCTCGAGGGCCCTGCC
GTCACCGTCGACACCGCCTGCTCCTCCTCCCTGGTCGCCCTGCACCTGGCCTGCCAGGCC
CTGCGCAACGGCGAGTGCTCACTCGCGCTGGCCGGTGGCGTGACGGTCATGGCCGTACCG
GGTCTGTTCACCGAGTTCAGCAGGCAGCGGGGCCTCGCACCCGACGGCCGCTGCAAGGCC
TTCGCCGCGGCGGCCGACGGCACGGGCTTCTCCGAAGGCGTGGGCGTCCTGCTGGTGGAG
CGGCTGTCTGACGCCCGCCGCAACGGCCACCAGGTGCTCGCCGTCGTCCGCGGCTCCGCC
GTCAACCAGGACGGCGCCAGCAACGGCCTCACCGCACCCAACGGCCCTTCGCAGCAACGC
GTGATCCGCCAGGCCCTCGCCAACGCCCGCCTCACCCCCGCCGACGTCGACGCCGTCGAG
GCGCACGGCACCGGAACCACGCTCGGCGACCCCATCGAAGCCCAAGCCCTCCTCGCCACC
TACGGGCAGAACCGCACGGAGGACCAGCCGCTGTGGCTCGGCTCGGTGAAGTCCAACATC
GGCCACACCCAGGCCGCCGCCGGTGTCGCGGGCGTCATCAAGATGGTCATGGCGCTGCGC
CACGAGGTACTGCCACAGACACTGCACGTCGACGCGCCCTCACCCCACATCGACTGGGCG
TCGGGCCACGTCTCCCTCCTCACCGAGGAACTGAAGTGGCCGGGGACGGACCGGCCGCGC
CGGGCCGGTGTGTCCTCCTTCGGCATCAGCGGCACCAACGCCCACGTCATCCTTGAAGCC
GCCCCGCCGGAGACCCCGCCCGCCGATGACGAGCCGGAGCGGGCGGCCGGACCGGTGCCG
TGGGTCCTCTCCGCCAGGACCAGGAAGGCCCTCCAGTCCCAGGCGGCCCGGTTGCTCACC
TTCACCGAGGAGACCCCGGACGTCTCCGTCGCCGCCGTGTCCTCCGCGCTGCTCGGCACG
CGTGCCGCCCTCGACCACCGGGCCGTCGTCCTGGCCTCGGACCGTGTCGAATTCTCACGA
CGCCTGCACGCACTGGCGTCCGGCAGGCCCGCGAGCGGAGTGACCACCGGGAGCGTGCGG
TCGGGGCGGCTGGCGTTCATGTTCACCGGCCAGGGCTCCCAACGCCTCGGCATGGGCCAC
GAGCTCTACGACACCGAACCCGCCTTCGCCGCAGCACTGGAAGAAGCCTTCGCAGCACTG
GACCCACACCTGCCACAGCCACTGCGCGACATCATCTTCGGCGACGACCCCGAGCTACTG
CAGCAAACCCAGTACGCACAACCCGCGCTGTTCGCCGTCGAGACCGCCCTGTACCGGCTC
CTCACCGACACCTACGGCCTGCGCCCCGACTACCTCCTCGGCCACTCCATCGGAGAACTC
ACCGCCGCCCACGCCGCCGGCGTCCTCACCCTGCCCGACGCCGCACGCCTGGTCACCGCC
CGCGGACACCTCATGCAAACCGCACCCACCGGCGGAACCATGATCGCCATCCAAGCCACG
GAAGACGAAATCACCGAACACCTGACCCCCGGGGTGGCTATCGCCGCCCTCAACTCCCCC
AGCAGCACAGTCATATCGGGTGACACCGCCGAGGCAGAAGCCATCGCGGACCGCTTCCGC
GCCCTCGGCCGCAAGACCAGCCGACTGAAGGTCTCCCACGCCTTCCACTCCCCCCACATG
GACCCCGTCCTCGACGACTTCCGGCAGATCGCCGCCACCCTCGACTACCACCCCGCCCGC
ATCCCCGTCATCTCCAACACCACCGGACAGCCCGCCGAAAACCTCACATCCCCCGACTAC
TGGACCCAACACATCCGCCGCCCCGTCCGCTTCACCGACAGCCTCCACCACCTCACCCAG
CAGGGTGTGACGACCTTCCTCGAACTCGGTCCCGATGCCACCCTCGCCATGCTCGCCGAC
GCCCACGCCACCGCCGTCCTGCGCAGGAACTCGTCCGAGCCGCAGGCCCTGCGTACGGCG
CTCGCCGAGCTGTGGGTCCACGGCGTTCCGGTGGCTTGGCCTCACTCGACCGACCGGCGC
GTGGATCTGCCGACCTACGCCTTCGACCACCAGCACTACTGGCTGGACGCGGCGCCCGGA
ACCTCCGCCACCTCCGTCCGCCCGTCGGTGCCGGTGGAGGCCGCCGACGCACCGCCCACG
CTCGCTGATCGGTTCGAGGGACTGGGCGCGGGCGAACGGACCTCCATGGCTGAGGAGTTG
GTCCGTGAGACGGTCGCTGTCGTCCTGGGGTTCGCCGACCCGGAGGACGTGGACGTGTCC
ACGCCGTTCATCAAGCTGGGCATCGACTCACTGACGGCGGTGGAACTGCGCAGGCGGCTG
GGCGGGTACACCGGCCTGGAGATCCCCGCCGTGGTCGTGTTCGAGCATCCGACCACCCAC
GACCTCGCCGAGTACCTGATCGAGGCGATGTCCGCGCCTCCGGGAGCGACGCCGGACGGC
GCTTCGGGTGCGCCTTCCGGCGTTCGGGCGACCGCCGCGGTGGACTCGCTCTCGGATCTC
TTCGTCCGGGCGTGTGGACGGGGGGAGTTCGCCGAGGCCCACCGTCTCGCGCTGGGGCTC
GCCGAGTACCGGCCCACCTTCGCGGGCGAGCTGGACGGGGCGCGGTCGCCGGACGTCGTA
CCGCTTCGTCGAGGTACGGCGTCGCCGGCCGTGGTGTGCTTCCCGTCGTTCGTCTGGAAG
CCCGACTTCCAGCAGTACCTGTCGCTGGCCCGGGAGTTGACCGAGGACCGGGACGTCGCG
GTGTCGGGGCTGCCCGGGTTCCGGCCCGGGGAGCCGTTGCCCGCTTCGGCGGAGGCGCTG
GCGCGGGCGCTGGCGGCCGTGGTGCGCCGGGTCGCCGGTGGGCGGCGGTGTGTTCTGCTC
GGTCACTCGTCGGGGGGCTATGTGGCGGCGGCGGTGGCGGCACAGCTGGAGGAGGCGGGC
GAGGCGCCCGCCGGGCTGGTGCTGGTCGACACGCCGTGGTGGGGTTCCGCGGGCGGGCTG
GGGTCGCAGGAGTGGCTTCCGGTGATCAACGAGATGCTGCTGGCGCGCGGTGCGGCTGCG
GGTGCGGAGGACAGCGACGCGGGTGACGCCTGGGTCACCGCGCGGGCCAAGTACTTCAGC
CTGGACTTCGCGATCGCGCCACGGGATGTGCGGACCCTGCTGCTGCGGCCGGCCGAACCG
CTGGCCGGTTCCGCCGCGGACGCCGGCTGGCGCGCCGAGTGGCGGCCAGGCACCACGGTC
GTCGAGGTGCCGGGGAACCACTTCTCGATGCTGGACAGCGCCCATGCCCAGGCATGCGCC
AGGGCGGTGGAGGGCTGGCTCGCCACTCTGGGATAG

[11] KS	35..411

[11] AT	566..868

[11] malonyl-CoA	753..757

[11] ACP	937..1007

[11] TE	1105..1307

[11] KS	103..1233

[11] AT	1696..2604

[11] malonyl-CoA	2257..2271

[11] ACP	2809..3021

[11] TE	3313..3921

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001031 Thioesterase (Domain) PF00975 Thioesterase IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 Ac_transferase_reg IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) SSF47336 ACP_like PF00550 PP-binding G3DSA:1.10.1200.10 ACP_like PS50075 ACP_DOMAIN IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 Acyl_Trfase/lysoPlipase IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Malonyl_transacylase_ACP-bd IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR018201 Beta-ketoacyl synthase, active site (Active_site) PS00606 B_KETOACYL_SYNTHASE IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 PKS_AT IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 PKS_PP IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 PKS_KS
SignalP	No significant hit
TMHMM	No significant hit

Lasal2_00210 Lasal2_00230