Teicp_00070 Teicp_00070   Teicp_00090 Teicp_00090

Teicp_00080 : CDS information

close this sectionLocation

Organism
StrainDSM 43866 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction9,591 / 9,052 / - [in whole cluster]
9,591 / 9,052 / - [in contig]
Locationcomplement(9052..9591) [in whole cluster]
complement(9052..9591) [in contig]
TypeCDS
Length540 bp (179 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)dihydrofolate reductase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF4
Reference
ACC
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
comment
DSM 43866株teicoplanin生合成gene clusterの同定論文。

tei orf4: Dihydrofolate reductase

配列解析から、Orf4, Orf22*, Orf25*はtyrosine生合成の代替ルートに関与し、tyrosineの高い需要を満たす助けとなると示唆されている。
Related Reference
ACC
Q6ZZK0
NITE
Teicp2_00140
PmId
[11386360] Teicoplanin biosynthesis genes in Actinoplanes teichomyceticus. (Antonie Van Leeuwenhoek. , 2000)
[14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004)
[15500981] Glycopeptide resistance determinants from the teicoplanin producer Actinoplanes teichomyceticus. (FEMS Microbiol Lett. , 2004)
comment
DSM 43866株でのtei orf4とAA配列完全一致。
UniProt 同一entry。

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[PMID: 11386360, 14702401]
ATCC 31121株teicoplanin生合成gene clusterの同定論文。

tcp ORF5: Unknown

ORF5はAmycolatopsis orientalis由来の機能不明タンパクと関連があるが、そのタンパクはcep clusterの外側にある。配列解析のみ。

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[PMID: 15500981](2004)
7つのtcp genes(tcp1-7に相当)を導入されたS. coelicolorは、teicoplaninとA40926への耐性を獲得した。

close this sectionSequence

selected fasta
>hypothetical protein [dihydrofolate reductase]
MSDVSGAGKVVVNRAMSLDGFIAGPGHAMDWIFEYLTGDMFPEVMASTGAMLIGRGTYEV
AKRMAGQGTPYDGGAQFVLTHEPPHEPDPAVTFLSCDIEEAVATARSAAGGKNLEILGAD
VAAQCLRRGLVDEILVYVVPVLLGDGVRFSPPSLDRIDLEPLSNTRSGAVTMLRFRVRK
selected fasta
>hypothetical protein [dihydrofolate reductase]
ATGAGCGATGTGAGCGGCGCCGGCAAAGTGGTCGTGAACAGGGCGATGTCGCTGGACGGG
TTCATCGCCGGTCCCGGCCACGCGATGGACTGGATCTTCGAATACCTGACTGGGGACATG
TTCCCGGAGGTTATGGCGTCCACGGGCGCCATGCTCATCGGCCGAGGCACGTACGAGGTC
GCCAAGCGTATGGCCGGCCAGGGCACCCCCTACGACGGGGGAGCACAGTTCGTCCTCACG
CACGAGCCTCCCCACGAGCCGGACCCTGCCGTCACGTTCCTCAGCTGTGACATCGAGGAA
GCCGTTGCCACGGCACGCAGCGCCGCGGGCGGCAAGAACTTGGAAATCCTCGGTGCTGAC
GTGGCCGCCCAGTGCCTGCGGCGCGGGCTCGTCGACGAGATCCTGGTGTATGTCGTACCG
GTGCTGCTCGGCGACGGTGTCCGCTTCTCGCCCCCAAGCCTCGACAGGATCGACCTGGAA
CCTCTCAGCAACACCCGGTCCGGCGCCGTCACGATGCTGCGCTTCCGCGTGCGAAAGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002734 Bacterial bifunctional deaminase-reductase, C-terminal (Domain)
[9-160] 3.09999999999998e-17 PF01872
PF01872   RibD_C
IPR024072 Dihydrofolate reductase-like domain (Domain)
[9-178] 4.5e-33 G3DSA:3.40.430.10
G3DSA:3.40.430.10   G3DSA:3.40.430.10
[8-178] 2.39999798157265e-29 SSF53597
SSF53597   SSF53597
SignalP No significant hit
TMHMM No significant hit
Teicp_00070 Teicp_00070   Teicp_00090 Teicp_00090
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