Teicp_00070 : CDS information

Location

Organism	Actinoplanes teichomyceticus
Strain	DSM 43866 (=NBRC 13999)
Entry name	Teicoplanin
Contig	AJ632270
Start / Stop / Direction	7,682 / 8,290 / + [in whole cluster] 7,682 / 8,290 / + [in contig]
Location	7682..8290 [in whole cluster] 7682..8290 [in contig]
Type	CDS
Length	609 bp (202 aa)

Annotation

Category	4.4 resistance
Product	putative D-alanyl-D-alanine dipeptidase
Product (GenBank)	dipeptidase
Gene	vanX
Gene (GenBank)
EC number	3.4.13.22
Keyword
Note
Note (GenBank)	ORF5
Reference	ACC Q6ZZK1 PmId [15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004) comment [PMID: 15113000](2004) DSM 43866株teicoplanin生合成gene clusterの同定論文。 tei orf5: D-Ala- D-Ala dipeptidase tei clusterにおいてteicoplanin耐性を与える遺伝子組織は、vancomycin耐性腸球菌(VRE)でグリコペプチド耐性を与えるvanHAXに似ている。orf5はD-Ala-D-Ala dipeptidaseをコードするvanXに相同である。配列解析のみ。
Related Reference	ACC Q6ZZK1 NITE Teicp2_00130 PmId [11386360] Teicoplanin biosynthesis genes in Actinoplanes teichomyceticus. (Antonie Van Leeuwenhoek. , 2000) [14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004) [15500981] Glycopeptide resistance determinants from the teicoplanin producer Actinoplanes teichomyceticus. (FEMS Microbiol Lett. , 2004) [17220405] Resistance to glycopeptide antibiotics in the teicoplanin producer is mediated by van gene homologue expression directing the synthesis of a modified cell wall peptidoglycan. (Antimicrob Agents Chemother. , 2007) comment DSM 43866株でのtei orf5とAA配列完全一致。 UniProt同一entry. --- [PMID: 11386360, 14702401] ATCC 31121株teicoplanin生合成gene clusterの同定論文。 tcp ORF4: D-Alanyl-D-alanine dipeptidase 配列解析のみ。vanHAX-like glycopeptide-resistance cassette (ORF2-4)は、産生株におけるteicoplanin耐性をいくらか与えることに関与しそう。 --- [PMID: 15500981](2004) 7つのtcp genes(tcp1-7に相当)を導入されたS. coelicolorは、teicoplaninとA40926への耐性を獲得した。 --- [PMID: 17220405](2007) teicoplaninの産生株A. teichomyceticus ATCC 31121における自己耐性の包括的研究。 vanHat, vanAat, vanXat genesはユニークなpolycistronic mRNAで同時転写されることが示された。 vancomycinまたはteicoplanineを添加してもvanXat転写物量にはほとんど影響がなかったことから、恒常的な転写が示唆された。(data not shown)
Related Reference	ACC Q9ZI81 NITE A4793_00330 PmId [9751644] Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function. (Chem Biol. , 1998) comment BLAST id79% Streptomyces toyocaensis NRRL 15009_vanXst D-ala-D-ala dipeptidase ・Streptomyces toyocaensis (StoVanX) ・Escherichia coli (EcoVanX) ・Synechocystis sp. strain PCC6803 (SynVanX) におけるvanX homologsを明らかにし、D,D-dipeptidase触媒活性を検証。 StoVanXは高効率なD-Ala-D-Ala dipeptidaseであり、D-Ala-D-lactate depsipeptide活性を欠く。

Sequence

>putative D-alanyl-D-alanine dipeptidase [dipeptidase]
MKDDFAFVDELVPGIRWDAKYATWDNFTGKPVDGYVANRIVGTKALCAALARAQEEAETL
GFGLLLWDGYRPQRAVDCFMRWAEQPEDGRKKLRHYPNIGRPEMFEKGYVATKSGHSRGS
TVDLTLYNLATGELASMGGDHDLMDPISHHGASGITPIEAKNREYLCSIMEGCGFARYDY
EWWHYTLKNEPYPDTYFDFPIV

>putative D-alanyl-D-alanine dipeptidase [dipeptidase]
ATGAAAGACGACTTCGCGTTCGTGGACGAGCTCGTGCCAGGCATACGCTGGGATGCCAAG
TACGCCACCTGGGACAATTTCACCGGCAAGCCGGTGGACGGTTACGTTGCGAATCGGATC
GTCGGAACGAAGGCGTTGTGCGCTGCCCTGGCGCGCGCCCAGGAAGAGGCCGAGACGCTT
GGCTTCGGTCTGCTTCTCTGGGATGGGTATCGGCCGCAACGCGCCGTGGACTGCTTCATG
CGCTGGGCGGAGCAGCCGGAGGACGGCCGCAAGAAGCTGCGGCACTACCCGAACATCGGC
AGGCCCGAGATGTTCGAGAAGGGGTATGTCGCCACCAAGTCCGGTCACAGCCGCGGCAGC
ACCGTCGACTTGACGCTCTACAACCTTGCCACCGGCGAACTGGCGTCGATGGGCGGCGAC
CACGACCTGATGGATCCGATCTCCCACCACGGGGCCAGCGGAATCACGCCGATCGAAGCG
AAGAACCGTGAATACCTCTGCTCGATCATGGAGGGCTGCGGATTCGCCAGGTACGACTAC
GAGTGGTGGCACTACACCCTGAAGAACGAACCCTATCCCGACACATATTTTGATTTTCCG
ATCGTGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000755 Peptidase M15D, VanX D-ala-D-ala dipeptidase (Family) PIRSF026671 AA_dipeptidase PF01427 Peptidase_M15 IPR009045 Hedgehog signalling/DD-peptidase zinc-binding domain (Domain) SSF55166 Hedgehog_sig_N G3DSA:3.30.1380.10 Hedgehog/DD-pept_Zn-bd
SignalP	No significant hit
TMHMM	No significant hit

Teicp_00060 Teicp_00080