Teicp_00170 Teicp_00170   Teicp_00190 Teicp_00190

Teicp_00180 : CDS information

close this sectionLocation

Organism
StrainDSM 43866 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction42,671 / 44,452 / + [in whole cluster]
42,671 / 44,452 / + [in contig]
Location42671..44452 [in whole cluster]
42671..44452 [in contig]
TypeCDS
Length1,782 bp (593 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative mannosyltransferase
Product (GenBank)mannossyltransferase
Gene
Gene (GenBank)
EC number2.4.1.-
Keyword
  • Dpg7
Note
Note (GenBank)
  • ORF3*
Reference
ACC
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
[27285718] Characterization of the Post-Assembly Line Tailoring Processes in Teicoplanin Biosynthesis. (ACS Chem Biol. , 2016)
comment
[PMID: 15113000](2004)
DSM 43866株teicoplanin生合成gene clusterの同定論文。

tei orf3*: Mannossyltransferase

配列解析のみ。dbv clusterのorf20を参考にして機能割り当てをしている。

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[PMID: 27285718](2016)
tei10*, tei3*, tei11*, tei13*, tei30*を不活化したmutantを作製し、その結果から組立てライン後の仕立て過程の反応の順番を提唱している。
Related Reference
ACC
Q6ZZJ0
NITE
Teicp2_00240
PmId
[14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004)
comment
DSM 43866株でのtei orf3*とAA配列完全一致。
UniProt同一entry.

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ATCC 31121株teicoplanin生合成gene clusterの同定論文。

tcp ORF15: Mannosyltransferase

dbv clusterでの提唱に基づき、機能割り当てしている。配列解析のみ。
ACC
Q7WZ71
NITE
A4092_00260
PmId
[12837387] The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species. (Chem Biol. , 2003)
comment
BLAST id62%, AA300-400にかけて大きなgapあり。
Nonomuraea sp. ATCC 39727_dbv20
Putative mannosyltransferase

Nonomuraea species ATCC39727由来A40926生合成gene clusterの同定論文。

dbv ORF20: mannosyltransferase

配列解析のみだが、ramoplanin gene clusterのORF19(ram19)、S. coelicolor homologとのalignmentから、protein mannosyltransferases familyに特有のmotifsを同定している。
PmId
[25878261] ( , )
comment
Actinoplanes sp. ATCC 33076由来ramoplanin生合成gene clusterにあるram29によってコードされるputative mannosyltransferaseの機能決定論文あり。

[PMID: 23892980](2013)
ramoplaninはdi-mannose部分のあるリポペプチド抗生物質である。ramo-orf29の不活化mutantでは、ramoplanin非産生だが、ramoplanin aglyconeを産生する。

[PMID: 25878261](2015)
ram29をdi-mannose部分を持たないenduracidin産生Streptomyces fungicidicusにおいて異種性発現し、monomannosylated enduracidin誘導体を産生させている。

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UniProtに該当entry見当たらないが、PMID: 12837387においてalignmentされているタンパクのひとつ。

close this sectionSequence

selected fasta
>putative mannosyltransferase [mannossyltransferase]
MVSETLTHPPSQDSAPESRPHRWAVWRSPAGQPAWARPALLGIALVAAVLYAWNLRLVDY
APRYADAVKSMSGSWRAFLYGAVDPEATYTLDKLAGSFVPQVVSAKIFGYHAWSLALPQV
IEGVIAVLVMYRVVRRWVGVVPGLLAAGIFTLTPVAASMFGHSMEDGALVMCLVLAVDAY
QRAVLEGRLRSLIGAGVWVGLGFQAKMVQAWMILPALAVGYLLTAPIELRRRVRHLGAAG
AVMLAVSLSWVMLYTLTPASDRPYISGTTNNNAFAMVFGYNGLGRVGINLPGAEEVNGRN
GVPAPGPLGGPEGEGQRGTVGTKAQDQRGTVGTKAQDQRGTVGTKAQDQRGTVGTKAQDQ
KGMVGPKAHGGPPMGPGALPEGYESKMESRADPMGWTKLFDGHLGVAIGWLFPLTLLALL
CGLWWRRRAERTDPVRGGLVMWGVWLVTFGFVFSASAVAHTAYVASLAPAIAAVSALGIV
MFGRAYRRGGRQGWILPLAVAVQLAWAAWLWSHYPNFLPWARWGTLLLGVAALVGLVLAR
VTRTASSALVAAALAVGVGVMLAAPATYAVSVLDPDYSGNSFDANAGPASGTA
selected fasta
>putative mannosyltransferase [mannossyltransferase]
GTGGTGTCCGAAACACTCACTCATCCGCCGTCCCAGGATTCCGCGCCCGAGAGCCGGCCT
CACCGCTGGGCGGTATGGCGCTCACCGGCCGGTCAGCCGGCGTGGGCCCGGCCCGCGCTG
CTGGGCATCGCGCTCGTCGCGGCGGTGCTGTATGCCTGGAACCTGCGGCTGGTCGACTAC
GCGCCGCGGTACGCGGACGCCGTCAAGAGCATGTCCGGGAGCTGGCGCGCGTTCCTGTAC
GGCGCCGTCGACCCGGAGGCGACGTACACGCTCGACAAGCTGGCCGGCTCCTTCGTGCCC
CAGGTCGTCTCGGCCAAGATCTTCGGTTATCACGCGTGGTCCCTGGCGTTGCCGCAGGTG
ATCGAGGGCGTGATCGCGGTGCTGGTGATGTACCGGGTCGTACGGCGGTGGGTGGGCGTG
GTGCCCGGCCTGCTCGCCGCCGGCATCTTCACCCTGACCCCGGTCGCCGCGTCCATGTTC
GGGCACAGCATGGAGGACGGCGCACTGGTCATGTGCCTGGTGCTCGCCGTCGACGCGTAT
CAGCGCGCGGTCCTGGAAGGGCGGCTCCGGTCACTGATCGGGGCCGGTGTCTGGGTCGGG
CTGGGCTTCCAGGCCAAGATGGTGCAGGCGTGGATGATTCTGCCCGCCCTGGCGGTCGGC
TACCTGCTGACCGCGCCGATCGAGCTGCGCCGCCGGGTGCGGCATCTCGGGGCCGCCGGG
GCGGTGATGCTGGCGGTGTCGCTGTCGTGGGTCATGCTCTACACCCTCACGCCGGCCAGC
GATCGGCCCTACATCAGTGGCACCACGAACAACAACGCCTTCGCCATGGTGTTCGGATAC
AACGGTCTCGGCCGTGTGGGCATCAACCTGCCCGGCGCCGAGGAGGTCAACGGTCGTAAC
GGCGTGCCGGCGCCCGGTCCGCTGGGCGGCCCGGAGGGCGAGGGCCAGAGGGGAACGGTC
GGCACGAAGGCCCAGGACCAGAGGGGAACGGTCGGCACGAAGGCCCAGGACCAGAGGGGA
ACGGTCGGCACGAAGGCCCAGGACCAGAGGGGAACGGTCGGCACGAAGGCCCAGGACCAG
AAGGGAATGGTCGGGCCGAAGGCCCATGGAGGACCGCCGATGGGACCGGGCGCGCTCCCC
GAGGGATACGAGTCCAAGATGGAGAGCAGGGCCGATCCGATGGGTTGGACCAAGCTGTTC
GACGGTCACCTCGGCGTCGCGATCGGCTGGCTCTTTCCGCTGACTCTGCTCGCCCTGCTG
TGCGGGTTGTGGTGGCGCCGCCGGGCCGAGCGCACCGATCCGGTGCGCGGCGGGCTGGTG
ATGTGGGGGGTGTGGCTGGTGACCTTCGGCTTTGTCTTCAGTGCGAGCGCTGTCGCGCAC
ACCGCCTACGTGGCCTCGCTGGCGCCGGCGATCGCGGCCGTCTCCGCCCTCGGCATCGTG
ATGTTCGGGCGGGCGTATCGGCGCGGCGGCAGGCAGGGCTGGATACTGCCGCTCGCGGTC
GCGGTCCAACTGGCCTGGGCCGCCTGGCTGTGGTCGCACTACCCGAACTTCCTCCCCTGG
GCGAGATGGGGCACGCTCCTGCTCGGCGTGGCCGCCCTTGTCGGGCTGGTCCTGGCGCGC
GTGACCAGGACCGCCTCCTCCGCGCTGGTCGCGGCCGCGCTCGCCGTCGGCGTCGGCGTC
ATGCTGGCCGCGCCCGCCACGTATGCCGTCTCGGTGCTCGATCCCGATTACTCCGGCAAC
TCCTTCGATGCCAACGCGGGACCGGCCTCGGGCACCGCGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM
[33-55] Transmembrane (o-i) [137-159] Transmembrane (i-o) [207-229] Transmembrane (o-i) [236-258] Transmembrane (i-o) [403-425] Transmembrane (o-i) [438-460] Transmembrane (i-o) [464-486] Transmembrane (o-i) [493-510] Transmembrane (i-o) [520-542] Transmembrane (o-i) [549-571] Transmembrane (i-o)
Transmembrane 10   
Teicp_00170 Teicp_00170   Teicp_00190 Teicp_00190
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