Teicp_00180 Teicp_00180   Teicp_00200 Teicp_00200

Teicp_00190 : CDS information

close this sectionLocation

Organism
StrainDSM 43866 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction44,746 / 46,692 / + [in whole cluster]
44,746 / 46,692 / + [in contig]
Location44746..46692 [in whole cluster]
44746..46692 [in contig]
TypeCDS
Length1,947 bp (648 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter ATP-binding/permease protein
Product (GenBank)ABC transporter
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • ORF4*
Reference
ACC
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
comment
DSM 43866株teicoplanin生合成gene clusterの同定論文。

tei orf4*: ABC transporter

配列解析のみ。細胞からのteicoplanin排出を担うと考えられている。
Related Reference
ACC
Q6ZZI9
NITE
Teicp2_00250
PmId
[11386360] Teicoplanin biosynthesis genes in Actinoplanes teichomyceticus. (Antonie Van Leeuwenhoek. , 2000)
[14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004)
comment
DSM 43866株でのtei orf4*とAA配列完全一致。
UniProt同一entry.

---
ATCC 31121株teicoplanin生合成gene clusterの同定論文。

tcp ORF16: ABC transporter

配列解析のみ。ORF34と共に排出機能に関与するとされている。
ACC
Q799B1
NITE
Balhi_00060
PmId
[17693715] The border sequence of the balhimycin biosynthesis gene cluster from Amycolatopsis balhimycina contains bbr, encoding a StrR-like pathway-specific regulator. (J Mol Microbiol Biotechnol. , 2007)
[17823795] The ABC transporter Tba of Amycolatopsis balhimycina is required for efficient export of the glycopeptide antibiotic balhimycin. (Appl Microbiol Biotechnol. , 2007)
comment
BLAST id80%
Amycolatopsis balhimycina_abc
Putative ABC transporter ATP-binding protein

---
[PMID: 17693715](2007)
グリコペプチド系抗生物質balhimycinの生合成gene clusterの追加報告。
新たにVanS, VanR, VanY, Bbr, Pdh, Tba, Dahpを同定している。

Tba(651aa) : ABC transporter ATP-binding protein

Tbaのpromoter領域にregulator Bbrが結合することが確認されている。

---
[PMID: 17823795](2007)
配列解析から、ABC-transporter様遺伝子によってコードされるTbaはexporterであると示唆された。Tbaに似ているものとして、CZA382.30(O52818)が挙げられている。

E. coliで発現、精製されたTbaは、ATPase活性を有する活性型であることが確認された。

tba不活化mutantは生育やグリコペプチド耐性に影響しなかったが、細胞外balhimycin濃度が減少し、細胞内balhimycin濃度は10倍高くなった。

close this sectionSequence

selected fasta
>putative ABC transporter ATP-binding/permease protein [ABC transporter]
MDVVLRFGGVSKNDDDPDPWQTLIGPGTLRRVLAYFRPHIGKVALFVLVAALESLVVVVV
PLLLMELVNGGILKDDAGVVASMAGLAAGLALVGALLQLVSAYISGQIGQGVSYDLRVQA
LRHVQQLPIAFFTRTQTGVLVGRLHTELIMAQQAFSQLLMAATSAVTVILVMAELFYLSW
LVAVVTLVLIPLFLVPWIYVGRVIRRRTGRLMDANTGLGGLLQERFNVQGAMLSKLFGRP
REEMAEYADRAERIRRIGVSLSVYGRLAFVMMALMASLATALVYGIGGGLVLAGAFQLGT
LVALATLLGRLFGPITQLSGMQETAQTVVVSFSRIFEVLDLKGLIEERPGAVALAKDTAP
EIEFDDVAFRYPTADEVSLRSLEYVRAERERGETTPEVLRGVSLHVPAGTLTALVGPSGA
GKSTLTHLVSRLYDPTGGVIRVGGHDLRDLTFDSLREAVGVVSQDAYFFHDTIRQNLLYA
RPTATEDELIEACRGAQIWDLIASLPGGFDTVTGDRGYRLSGGEKQRLAIARLLLKAPTI
VVLDEATAHLDSESEAAVQRALKTALHNRTSLVIAHRLSTIREADQILVIDDGRVRERGT
HDELLAEGGLYAELYHTQFAKAGSDAAEPASDAAELHHREPEPVHGGG
selected fasta
>putative ABC transporter ATP-binding/permease protein [ABC transporter]
GTGGACGTGGTGTTGCGCTTCGGGGGGGTGTCGAAGAACGATGACGACCCCGATCCCTGG
CAGACCCTGATCGGGCCGGGGACGCTGCGCAGAGTGCTCGCCTACTTCCGCCCGCACATC
GGCAAGGTGGCGCTCTTCGTTCTCGTCGCCGCGCTGGAGTCGCTTGTCGTCGTCGTAGTT
CCGTTGTTGCTGATGGAACTGGTCAACGGCGGCATCCTCAAGGACGACGCCGGGGTCGTG
GCATCGATGGCCGGCCTGGCCGCCGGCCTCGCCCTGGTGGGCGCGCTGTTGCAGCTGGTG
TCAGCCTATATTTCCGGGCAGATCGGGCAGGGGGTCAGCTACGACCTCCGGGTTCAGGCG
CTGCGTCACGTTCAGCAGCTGCCGATCGCGTTCTTCACCCGTACCCAGACCGGGGTGCTG
GTGGGCCGGCTGCACACGGAACTGATCATGGCGCAGCAGGCGTTCAGCCAGCTCCTGATG
GCAGCCACCAGCGCCGTCACGGTGATCCTGGTGATGGCCGAGCTGTTCTACCTGTCATGG
CTTGTCGCCGTGGTCACCCTGGTGCTGATTCCGCTGTTCCTCGTGCCCTGGATCTACGTG
GGCCGGGTGATCCGGCGGCGCACCGGACGGCTGATGGACGCGAATACCGGCCTTGGTGGA
CTCCTCCAGGAGCGGTTCAACGTTCAGGGGGCGATGCTCTCCAAACTCTTCGGCCGTCCC
CGCGAGGAGATGGCCGAGTACGCCGATCGTGCCGAGCGCATCCGCCGCATCGGCGTGAGT
CTCTCGGTGTACGGGCGGCTCGCCTTCGTCATGATGGCGCTGATGGCCTCGCTCGCCACG
GCGCTCGTCTACGGCATCGGTGGCGGGCTCGTGCTCGCCGGGGCGTTCCAGCTCGGCACG
CTCGTCGCCCTCGCCACTCTGCTCGGGCGGTTGTTCGGGCCGATCACGCAGCTGTCCGGG
ATGCAGGAAACCGCGCAGACCGTCGTGGTGAGCTTCTCCCGGATCTTCGAGGTGCTCGAT
CTGAAGGGGCTGATCGAGGAGCGTCCCGGTGCGGTCGCGCTCGCCAAGGACACGGCGCCG
GAGATCGAGTTCGACGACGTGGCGTTCCGCTATCCGACCGCCGACGAGGTCTCGCTGCGC
TCGCTGGAGTACGTACGCGCCGAACGGGAGCGCGGGGAGACGACGCCGGAGGTGCTGCGC
GGTGTCAGCCTGCACGTGCCGGCCGGCACCCTCACCGCGCTCGTCGGCCCGTCCGGCGCC
GGCAAGAGCACGCTCACCCACCTGGTGTCACGGCTGTACGACCCGACCGGCGGGGTCATT
CGCGTCGGTGGGCACGATCTGCGCGACCTCACCTTCGACTCGCTGCGCGAAGCGGTGGGC
GTGGTCAGCCAGGACGCCTACTTCTTCCACGACACGATCCGGCAGAACCTGCTCTACGCC
CGCCCCACCGCCACCGAGGACGAACTGATCGAGGCATGTCGAGGGGCCCAGATCTGGGAC
CTGATCGCCTCGCTCCCGGGCGGGTTCGACACCGTCACCGGCGATCGTGGCTACCGCCTG
TCGGGCGGCGAGAAGCAACGCCTGGCCATCGCCCGGCTGCTGCTGAAGGCGCCGACGATC
GTCGTGCTCGACGAAGCCACCGCTCACCTGGACTCCGAGTCCGAGGCCGCCGTGCAGCGG
GCACTCAAGACCGCCCTGCACAACCGCACCTCGTTGGTGATCGCCCACCGGTTGTCCACG
ATTCGCGAGGCCGACCAGATCCTCGTGATCGACGACGGACGGGTCCGGGAGCGCGGAACG
CACGACGAGCTGCTGGCCGAGGGGGGTCTGTACGCGGAGCTTTACCACACGCAGTTCGCC
AAGGCCGGAAGCGACGCAGCCGAACCGGCGAGCGACGCAGCCGAGCTGCACCACAGGGAG
CCCGAGCCGGTGCACGGCGGAGGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001140 ABC transporter, transmembrane domain (Domain)
[46-314] 4.6e-21 PF00664
PF00664   ABC_membrane
IPR003439 ABC transporter-like (Domain)
[379-617] PS50893
PS50893   ABC_TRANSPORTER_2
[423-548] 8.19999999999997e-22 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
[408-594] 1.20000117458134e-18 SM00382
SM00382   AAA
IPR011527 ABC transporter, transmembrane domain, type 1 (Domain)
[16-349] 2.99998750445706e-62 SSF90123
SSF90123   ABC_TM_1
IPR017871 ABC transporter, conserved site (Conserved_site)
[520-534] PS00211
PS00211   ABC_TRANSPORTER_1
IPR017940 ABC transporter, integral membrane type 1 (Domain)
[44-327] PS50929
PS50929   ABC_TM1F
SignalP No significant hit
TMHMM
[43-65] Transmembrane (i-o) [80-102] Transmembrane (o-i) [154-173] Transmembrane (i-o) [178-200] Transmembrane (o-i) [263-285] Transmembrane (i-o) [290-312] Transmembrane (o-i)
Transmembrane 6   
Teicp_00180 Teicp_00180   Teicp_00200 Teicp_00200
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