Teicp_00190 Teicp_00190   Teicp_00210 Teicp_00210

Teicp_00200 : CDS information

close this sectionLocation

Organism
StrainDSM 43866 (=NBRC 13999)
Entry nameTeicoplanin
Contig
Start / Stop / Direction47,098 / 48,273 / + [in whole cluster]
47,098 / 48,273 / + [in contig]
Location47098..48273 [in whole cluster]
47098..48273 [in contig]
TypeCDS
Length1,176 bp (391 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)P450 monooxygenase
GeneoxyA
Gene (GenBank)
EC number
Keyword
  • 3rd cross-linking
  • D-O-E ring
Note
Note (GenBank)
  • ORF5*
Reference
ACC
PmId
[15113000] Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. (Chem Biol. , 2004)
[26820384] Structure of OxyA tei: completing our picture of the glycopeptide antibiotic producing Cytochrome P450 cascade. (FEBS Lett. , 2016)
comment
[PMID: 15113000](2004)
DSM 43866株teicoplanin生合成gene clusterの同定論文。

tei orf5*: P450 monooxygenase

遺伝子破壊実験がなされているbalhimycin生合成genesとの配列比較から、Orfs 5*, 7*, 9* はそれぞれ OxyA, OxyB, OxyC に関連があり、残基2-4, 4-6, 5-7間の架橋を担うと提唱している。

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[PMID: 26820384](2016) abstract
OxyAteiの構造解析。
Related Reference
ACC
Q6ZZI8
NITE
Teicp2_00270
PmId
[11386360] Teicoplanin biosynthesis genes in Actinoplanes teichomyceticus. (Antonie Van Leeuwenhoek. , 2000)
[14702401] Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus. (Microbiology. , 2004)
[25686610] X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. (Nature. , 2015)
[26549530] Sequential In Vitro Cyclization by Cytochrome P450 Enzymes of Glycopeptide Antibiotic Precursors Bearing the X-Domain from Nonribosomal Peptide Biosynthesis. (Angew Chem Int Ed Engl. , 2015)
[27213615] Regulation of the P450 Oxygenation Cascade Involved in Glycopeptide Antibiotic Biosynthesis. (J Am Chem Soc. , 2016)
comment
DSM 43866株でのtei orf5*とAA配列完全一致。
UniProt同一entry.

---
[PMID: 11386360, 14702401]
ATCC 31121株teicoplanin生合成gene clusterの同定論文。

tcp ORF18: OxyA, cross-linking aa 2-4

balhimycin生合成cluster gene(OxyA)と高い相同性があることから、AA2-4間の架橋形成が割り当てられている。

---
[PMID: 25686610](2015)
SUPPLEMENTARY INFORMATION内のリストでATCC 31121株由来UniProt entryの記載があるが、同グループの他論文(PMID: 20974107, 25358800, 25586301)ではDSM43866株を使用してATCC 31121株由来のgene nameを採用している混乱があるため、同様の可能性あり。

X-domainの結晶構造解析や相互作用の実験から、X-domainがcytochrome P450(oxy genesでコードされる)による架橋形成に関与することがわかった。OxyA-CはX-domainと相互作用するが、OxyEはしない。OxyAとOxyBによる架橋形成には、X-domainを含む基質を必要とする。

---
[PMID: 26549530](2015) abstract + supporting information
PMID: 25686610の続報。同様にsupporting informationでATCC 31121株由来entriesが採用されている。
ACC
Q8KLL7
NITE
A4793_00090
PmId
[17884639] The biosynthesis of teicoplanin-type glycopeptide antibiotics: assignment of p450 mono-oxygenases to side chain cyclizations of glycopeptide a47934. (Chem Biol. , 2007)
comment
BLAST id81%
Streptomyces toyocaensis
Cytochrome P450(StaF)
teicoplanin-type glycopeptide A47934生合成cluster gene

---
[PMID: 17884639](2007)
架橋形成に関するP450 monooxygenasesの調査。

mutants(ΔstaF, ΔstaG, ΔstaH, ΔstaJ）に蓄積した中間体の解析により、各架橋形成は特異的なoxygenaseへ明白に割り当てられ、環化の順番も決定された。
1) C-O-D by StaH
2) F-O-G by StaG
3) D-O-E by StaF
4) AB by StaJ

close this sectionSequence

selected fasta
>cytochrome P450 [P450 monooxygenase]
MFEEINVVRAAELHRRDRFDPVPQLRSLMAEGPLTTLGTEESPGGRTAWLATGYDEIRQV
LSSDDFSARLLYGGTAAGITWPGFLTQYDPPEHTRLRRMVAPAFAVRRMQKFQPQVERVV
QDSLDAIEALGGPVDFVPRFGWSVATTATCDFLGIPRDDQADLARSLHASRTERSGKRRT
AAGNKFMTYMNKMTARTRRDPGDDMFGVVVREYGDEITDAELTGVAAFVMGAGADQVARF
LAAGAWLMADDPEQFALLREKPDTVPDWLDEVIRYLTTDEKTHPRVATDDVRIGDHLIKA
GDTVTCSLLAANRRNFPRPEDRFDITRVRPEHLAFGHGIHHCLGRSLAELVFRTAIPALA
HRFPTLRLAEPHREIRLGPPPFDVEALLLDW
selected fasta
>cytochrome P450 [P450 monooxygenase]
ATGTTCGAGGAGATCAACGTTGTCCGGGCCGCGGAGCTGCACCGGAGGGACCGATTCGAT
CCGGTGCCACAGCTGCGCTCGCTGATGGCCGAGGGCCCGTTGACCACGCTGGGCACCGAG
GAGTCTCCCGGAGGCCGGACGGCGTGGCTCGCCACCGGGTACGACGAGATCCGGCAGGTT
CTCAGCTCCGACGACTTCAGCGCACGGCTGCTCTACGGGGGAACCGCTGCCGGCATCACC
TGGCCCGGTTTCCTCACCCAGTACGACCCGCCCGAGCACACCCGACTGCGCCGCATGGTG
GCGCCCGCCTTCGCCGTCCGACGGATGCAGAAGTTCCAGCCGCAGGTCGAGCGGGTCGTC
CAGGACAGTTTGGACGCCATCGAGGCGCTCGGCGGACCGGTCGACTTCGTCCCCCGTTTC
GGGTGGTCGGTGGCGACGACGGCGACCTGCGACTTCCTCGGCATTCCGCGCGACGATCAG
GCGGATCTGGCGCGCAGCCTCCATGCCAGCCGAACCGAACGGTCAGGCAAGCGGCGTACC
GCGGCAGGCAACAAGTTCATGACGTACATGAACAAGATGACGGCTCGCACCCGCCGCGAT
CCCGGTGACGACATGTTCGGTGTCGTGGTACGCGAGTACGGCGACGAGATCACCGACGCG
GAGCTGACCGGCGTCGCCGCGTTCGTCATGGGCGCGGGCGCCGACCAGGTGGCCCGTTTC
CTCGCGGCCGGCGCGTGGCTGATGGCCGACGATCCCGAGCAGTTCGCACTGCTGCGGGAG
AAGCCGGACACCGTCCCGGATTGGCTGGACGAGGTGATTCGGTATCTGACCACCGATGAG
AAGACCCATCCGCGCGTCGCGACGGACGACGTGCGCATCGGCGATCACCTCATCAAGGCC
GGCGATACCGTCACGTGCTCCCTGCTGGCTGCCAACCGCCGCAACTTCCCGCGCCCGGAG
GATCGATTCGACATCACCCGGGTCCGGCCGGAGCACCTGGCGTTCGGGCACGGCATCCAT
CACTGCCTGGGCAGGTCTCTGGCCGAGCTGGTGTTCCGGACGGCGATTCCGGCCCTGGCA
CACCGCTTCCCCACGCTGAGGCTGGCCGAGCCGCACCGAGAGATCAGATTGGGGCCGCCG
CCGTTCGACGTGGAAGCCCTGCTGCTCGACTGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[14-378] 6.20000000000009e-81 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[269-363] 9e-11 PF00067
PF00067   p450
[18-391] 4.69998262513189e-74 SSF48264
SSF48264   Cytochrome_P450
IPR002397 Cytochrome P450, B-class (Family)
[88-99] 2.80000504261099e-34 PR00359 [135-151] 2.80000504261099e-34 PR00359 [152-167] 2.80000504261099e-34 PR00359 [188-210] 2.80000504261099e-34 PR00359 [267-278] 2.80000504261099e-34 PR00359 [285-312] 2.80000504261099e-34 PR00359 [333-342] 2.80000504261099e-34 PR00359 [342-353] 2.80000504261099e-34 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[335-344] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Teicp_00190 Teicp_00190   Teicp_00210 Teicp_00210
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