Balhi_00180 : CDS information

Location

Organism	Amycolatopsis balhimycina
Strain	DSM 5908
Entry name	Balhimycin
Contig	Y16952
Start / Stop / Direction	44,087 / 45,313 / + [in whole cluster] 44,087 / 45,313 / + [in contig]
Location	44087..45313 [in whole cluster] 44087..45313 [in contig]
Type	CDS
Length	1,227 bp (408 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-3-amino-4-keto-2,3,6-trideoxy-D-glucose C-3 C-methyltransferase
Product (GenBank)	putative C-3 methyl transferase
Gene
Gene (GenBank)	dvaC
EC number	2.1.1.-
Keyword	dehydrovancosamine
Note
Note (GenBank)
Reference	ACC Q939Y7 PmId [10390204] Identification and analysis of the balhimycin biosynthetic gene cluster and its use for manipulating glycopeptide biosynthesis in Amycolatopsis mediterranei DSM5908. (Antimicrob Agents Chemother. , 1999) comment Amycolatopsis mediterranei DSM5908からグリコペプチド系抗生物質balhimycinの生合成に関する9,882bpのDNA断片を同定。そこにoxyA-bgtfCまで7つのcomplete genesと1つのincomplete geneを同定した。 incomplete gene orfX' はタンパクのN末部分118aaをコードする。 A82846B(chloroeremomycin)生合成gene clusterのORF14と、vancomycin生合成gene clusterのhydroxylase B、daunorubicin/doxorubicinの生合成酵素DnrXに似ている。
Related Reference	ACC P96561 NITE Chlor_00150 PmId [11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000) comment BLAST id93% Amycolatopsis orientalis (Nocardia orientalis) PCZA361.22 ORF14(EvaC)に相当するentry. dTDP-4-keto-6-deoxy-D-glucoseからdTDP-L-epivancosamineを生合成する5つの酵素を、E.coliで発現・精製し、特徴づけしている。 ORF23はdTDP-6-deoxy-4-keto-D-glucoseを基質として、2,6-dideoxy-3,4-diketo-dTDP sugarの分解化合物をもたらした。ORF23とORF25の組み合わせは、C-3アミノ基がequatorialなdTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseをもたらした。さらにORF14を加えた組み合わせで補助基質としてSAMを使用すると、C-3メチル基がequatorialなdTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-uloseをもたらした。また、ORF14によるdTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseのC-methylationが、1.14ppmでのmethyl peakによって確認された。すべての結果から提唱されている経路は次の通り。 dTDP-4-keto-6-deoxy-D-glucose ↓ ORF23(EvaA): C-2 deoxygenation ↓ ORF25(EvaB): C-3 amination dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-ulose ↓ ORF14(EvaC): C-3 methylation dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-ulose ↓ ORF26(EvaD): C-5 epimerization ↓ ORF24(EvaE): C-4 ketoreduction dTDP-L-epivancosamine

Sequence

>putative dTDP-3-amino-4-keto-2,3,6-trideoxy-D-glucose C-3 C-methyltransferase [putative C-3 methyl transferase]
MSTTSQCRICDGTVHEFIDFGRQPLSDAFVAPGAEKGEFFFRLATGICDSCTMVQLMEEV
PRDLMFHEAYPYLSSGSAVMRTHFHELAKHLLATELTGEDPFIVELGCNDGIMLKAVADA
GVRQLGVEPSGSVADLAAAKGIRVRKDFFEEATAADIRENDGPADVIYAANTLCHIPYMD
SILKGVTKLLGPNGVFVFEDPYLGDIVERTSFDQIYDEHFFLFTARSVQEMARRNGLELV
DVERIPVHGGEVRYTLALAGARKPSEAVAELLAWEAERKLAEYATLERFATDVKKIKEDL
IALLTKLRAEGKRVVGYGATAKSATVTNFCGITPDLVEFISDTTPAKQGKLSPGQHIPVR
EYGEFAGNHPDYALLFAWNHADEIMNVEQAFRDAGGQWILYVPNVHVS

>putative dTDP-3-amino-4-keto-2,3,6-trideoxy-D-glucose C-3 C-methyltransferase [putative C-3 methyl transferase]
ATGTCGACCACGTCCCAGTGCCGTATCTGTGACGGCACTGTCCACGAGTTCATCGACTTC
GGACGCCAGCCGCTCTCGGACGCGTTCGTGGCTCCCGGCGCGGAAAAGGGTGAGTTCTTC
TTCCGCCTTGCCACCGGCATCTGCGATTCCTGCACGATGGTGCAGCTGATGGAGGAAGTC
CCGCGGGACCTGATGTTCCACGAGGCCTACCCCTACCTGTCGTCGGGTTCGGCCGTCATG
CGCACGCACTTCCACGAGCTGGCCAAGCACCTGCTGGCCACGGAGCTGACCGGCGAGGAC
CCGTTCATCGTCGAGCTCGGCTGCAACGACGGCATCATGCTCAAGGCCGTGGCCGACGCG
GGGGTGCGCCAGCTCGGCGTCGAACCCTCCGGCAGTGTCGCGGATCTGGCGGCAGCCAAG
GGGATCCGCGTCCGCAAGGACTTCTTCGAAGAGGCGACGGCCGCCGACATCCGCGAGAAC
GACGGCCCCGCGGACGTGATCTACGCGGCCAACACACTGTGCCACATCCCTTACATGGAC
TCGATCCTGAAGGGCGTCACCAAGCTGCTCGGCCCGAACGGCGTGTTCGTCTTCGAGGAC
CCGTACCTCGGCGACATCGTGGAGCGCACGTCGTTCGACCAGATCTACGACGAGCATTTC
TTCCTCTTCACGGCGCGCTCGGTCCAGGAGATGGCCCGGCGCAACGGCCTCGAGCTCGTG
GACGTCGAGCGCATTCCGGTGCACGGCGGCGAGGTCCGCTACACCCTGGCCCTGGCCGGC
GCTCGCAAGCCGTCCGAGGCCGTGGCGGAGCTCCTGGCCTGGGAGGCGGAGCGCAAGCTG
GCGGAGTACGCCACGCTGGAACGTTTCGCCACCGACGTGAAGAAGATCAAGGAAGACCTG
ATCGCGCTGCTGACCAAGCTCCGTGCCGAAGGCAAGCGCGTCGTCGGCTACGGCGCGACG
GCCAAGAGTGCCACGGTGACCAACTTCTGCGGCATCACCCCGGACCTGGTCGAGTTCATC
TCGGACACGACACCGGCCAAGCAGGGCAAGCTCAGCCCGGGACAGCACATCCCGGTCCGC
GAGTACGGGGAATTCGCCGGCAACCACCCGGACTACGCCCTGCTGTTCGCCTGGAACCAC
GCCGACGAGATCATGAACGTGGAACAGGCCTTTCGTGACGCCGGCGGTCAGTGGATCCTT
TACGTGCCGAACGTGCACGTGAGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013217 Methyltransferase type 12 (Domain) PF08242 Methyltransf_12 IPR013630 Methyltransferase putative zinc binding domain (Domain) PF08421 Methyltransf_13 IPR013691 C-methyltransferase (Domain) PF08484 Methyltransf_14
SignalP	No significant hit
TMHMM	No significant hit

Balhi_00170 Balhi_00190