Balhi_00180 Balhi_00180   Balhi_00200 Balhi_00200

Balhi_00190 : CDS information

close this sectionLocation

Organism
StrainDSM 5908
Entry nameBalhimycin
Contig
Start / Stop / Direction45,409 / 46,233 / + [in whole cluster]
45,409 / 46,233 / + [in contig]
Location45409..46233 [in whole cluster]
45409..46233 [in contig]
TypeCDS
Length825 bp (274 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number
Keyword
Note
  • This ORF product lacks a metal binding site (histidine residue) required for deacetylase activity.
Note (GenBank)
  • orf2
Reference
ACC
PmId
[18482700] The role of cep15 in the biosynthesis of chloroeremomycin: reactivation of an ancestral catalytic function. (Chem Biol. , 2008)
comment
Amycolatopsis balhimycina
Putative uncharacterized protein

このORFを同定した論文は不明。

---
[PMID: 18482700](2008)
teicoplanin cluster orf2*と似ていて紛らわしいので、balhimycin cluster orf2 を bal2 としてこの論文では扱っている。

PMID: 17044690でCep15(= chloroeremomycin Orf15)はdTDP-glucose形成を触媒するglucose-1-phosphate thymidyltransferaseとして機能すると提唱されているが、このとき見られた低いnucleotidyltransferase活性はホストのE.coliに由来すると考えられた。よって精製したCep15で想定されたnucleotidyltransferase or deacetylaseの触媒活性を確認。Cep15はどちらの活性も持たず、chloroeremomycin生合成で明らかな役割を持たない。

teicoplanin産生株のorf2* in-frame deletion mutantはteicoplaninを産生しなくなる。cep15の代わりに遺伝子操作しやすいbal2のin-frame deletion mutantを作成したが、wild株と変わらずbalhimycinを産生した。

deacetylasesとわかっているOrf2* and Dbv21と配列比較したところ、Cep15とBal2はmetal-binding Hisの代わりにAsnを持っていた。Orf2*のHisをAsnに置換すると不活性になった。逆にCep15のN164H mutantは活性のあるdeacetylaseとなった。
Related Reference
ACC
O52804
NITE
Chlor_00160
PmId
[18482700] The role of cep15 in the biosynthesis of chloroeremomycin: reactivation of an ancestral catalytic function. (Chem Biol. , 2008)
comment
BLAST id82%
Amycolatopsis orientalis (Nocardia orientalis)
PCZA361.23

Cep15に相当するentry.
実験内容は上記参照。

close this sectionSequence

selected fasta
>hypothetical protein [hypothetical protein]
MPQDLDADRILAISPHLDDAVLSFGAGLARAAQAGAKVTVHTVFAGTAAPPYSPAAERLH
AIWELSPDQDASLRRRDEDIAALDHLGVDYRHGRFLDAIYRKLPDGRWLADNVPGRQKLA
IGRQSPQGDPELFSAVRADIESIVEEYAPALILTCAAGNGHVDNEIARDAALFVAYEKGI
RVRLWEDLPHAMFAEGAAELPDGFRLGPPDFGSVEPEARARKFEALRLYSSQMLMLHGPE
KDFFAQLDGHARKSAPGGGYGETTWPVVSREDNG
selected fasta
>hypothetical protein [hypothetical protein]
ATGCCTCAAGACCTCGACGCGGACCGGATTCTGGCGATATCCCCGCATTTGGACGACGCG
GTTCTTTCCTTCGGTGCCGGCCTCGCCCGTGCGGCGCAGGCCGGCGCGAAGGTGACCGTT
CACACGGTGTTCGCCGGGACCGCGGCGCCCCCTTATTCGCCGGCGGCGGAGCGGCTGCAC
GCGATCTGGGAGCTCTCACCGGATCAAGACGCGTCGCTCCGCCGCCGGGACGAAGACATC
GCCGCGCTCGACCACCTGGGCGTCGACTACCGGCACGGCCGGTTCCTCGACGCCATCTAC
CGCAAGCTGCCGGACGGCCGATGGCTGGCCGACAACGTGCCGGGCCGCCAGAAGCTGGCC
ATCGGACGGCAATCGCCGCAGGGCGATCCGGAGCTGTTCTCCGCGGTCCGGGCGGACATC
GAGTCGATCGTCGAAGAGTACGCCCCGGCGCTGATCCTGACCTGCGCGGCAGGCAACGGT
CATGTCGACAACGAGATCGCGCGGGATGCCGCGCTGTTCGTCGCGTACGAGAAGGGCATC
CGGGTTCGGCTGTGGGAAGACCTTCCGCACGCGATGTTCGCGGAGGGCGCCGCCGAACTG
CCGGACGGATTCCGGCTGGGGCCGCCCGATTTCGGTTCCGTCGAACCGGAGGCACGGGCG
CGGAAATTCGAAGCGCTGCGGCTCTACTCGTCGCAGATGCTGATGCTGCACGGGCCGGAA
AAGGATTTCTTCGCTCAGCTGGACGGGCATGCCCGGAAGAGTGCACCGGGTGGTGGATAC
GGCGAAACGACCTGGCCGGTTGTCTCTCGCGAAGACAACGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003737 N-acetylglucosaminyl phosphatidylinositol deacetylase (Family)
[11-171] 9.9e-21 PF02585
PF02585   PIG-L
IPR024078 Putative deacetylase LmbE-like domain (Domain)
[6-250] 9.19998414420354e-39 SSF102588
SSF102588   SSF102588
[8-241] 1e-24 G3DSA:3.40.50.10320
G3DSA:3.40.50.10320   G3DSA:3.40.50.10320
SignalP No significant hit
TMHMM No significant hit
Balhi_00180 Balhi_00180   Balhi_00200 Balhi_00200
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