Balhi_00270 : CDS information

Location

Organism	Amycolatopsis balhimycina
Strain	DSM 5908
Entry name	Balhimycin
Contig	Y16952
Start / Stop / Direction	54,879 / 56,237 / + [in whole cluster] 54,879 / 56,237 / + [in contig]
Location	54879..56237 [in whole cluster] 54879..56237 [in contig]
Type	CDS
Length	1,359 bp (452 aa)

Annotation

Category	5.1 general function
Product	putative transporter
Product (GenBank)	putative antiporter
Gene
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in balhimycin biosynthesis and resistance.
Note (GenBank)	orf7
Reference	ACC Q939X8 PmId [21690280] Self-resistance and cell wall composition in the glycopeptide producer Amycolatopsis balhimycina. (Antimicrob Agents Chemother. , 2011) comment balhimycin生合成gene cluster内でグリコペプチド耐性に関与するかもしれない遺伝子として、orf7, vnlR, vnlS, vanYbを同定。 orf7はNa+/H+ antiporter geneに50%以上の相同性あり。しかしorf7のin-frame deletion mutantは、balhimycinの産生と耐性に影響しなかった。
Family/Domain	FD IPR006153 comment [IPR006153] Cation/H+ exchanger (Domain) Molecular Function GO:0015299 solute:proton antiporter activity

Sequence

>putative transporter [putative antiporter]
MLHTFAAAVAPVAPIAAHSLLVFLLQIGLLLLLAVVLGRLAGRFGMPAVVGELFVGVILG
PSLLGWAAPGLHSWLFPAVAEQYHLLDAVGQVGVLLLVGLTGVQMDMGLARKRGLTAAGV
SIGGLVLPLGLGIGAGYLLPKVLVPEGTDVTVFAMFLGVALCVSAIPVIAKTLIDMKLLH
RNIGQLTLTAGMVDDVFGWFMLSVVSAMAVNAVSAGTVLTSLAYLVAILAFCFTLGRPLA
RGVLRVAAKSDGPGLTVATVVVLIFLAAAGTQALGLEAVFGAFLCGILLGTAGKVDPAKL
APLRTVVLSGLAPLFFATAGLRMDLTALTHPVVLLTGLVVLALAIAGKFAGAFAGARLSG
LNKWEGLALGAGLNARGVIQVVVAMVGLRLGILSVEVYTIIILVAIVTSLMASPILRFAM
SRVEQTAEEQVRENEHRAWNTHPAANPQEQSL

>putative transporter [putative antiporter]
ATGCTGCACACCTTTGCCGCGGCGGTCGCGCCGGTCGCACCGATCGCCGCGCACAGTCTC
CTGGTCTTCCTGCTGCAGATCGGCTTGCTGCTCCTGCTCGCCGTCGTGCTCGGCCGGCTG
GCCGGCCGGTTCGGGATGCCCGCGGTCGTCGGTGAGCTGTTCGTCGGGGTGATCCTCGGT
CCGTCGCTGCTGGGCTGGGCGGCGCCGGGCCTGCACAGCTGGCTGTTCCCGGCCGTCGCC
GAGCAGTACCACCTGCTCGACGCCGTCGGCCAGGTCGGCGTCCTGCTGCTGGTCGGCCTC
ACCGGCGTGCAGATGGACATGGGGCTGGCCCGCAAGCGCGGCCTCACCGCGGCCGGGGTC
AGCATCGGCGGCCTGGTCCTTCCGCTCGGCCTGGGGATCGGCGCGGGTTACCTGCTGCCG
AAGGTGCTCGTTCCGGAGGGCACCGACGTCACCGTCTTCGCGATGTTCCTCGGCGTGGCC
CTGTGCGTCAGCGCCATCCCGGTCATCGCCAAGACCCTCATCGACATGAAACTGCTGCAC
CGCAACATCGGGCAGCTCACGCTCACCGCCGGCATGGTCGACGACGTGTTCGGCTGGTTC
ATGCTGTCCGTCGTCAGCGCGATGGCGGTCAACGCGGTCTCCGCCGGCACCGTGCTCACT
TCGCTGGCCTACCTGGTCGCCATCCTCGCCTTCTGTTTCACCCTCGGCCGTCCGCTGGCC
CGGGGTGTGCTCCGCGTCGCGGCCAAGTCCGACGGTCCCGGGCTCACCGTCGCCACCGTC
GTCGTCCTGATCTTCCTCGCCGCGGCCGGTACGCAGGCGCTCGGCCTGGAGGCGGTCTTC
GGCGCCTTCCTCTGCGGCATCCTGCTCGGGACGGCGGGCAAGGTGGATCCGGCCAAGCTC
GCCCCCCTGCGCACGGTCGTCCTGTCGGGACTCGCCCCCCTCTTCTTCGCCACGGCCGGG
TTGCGGATGGACCTCACCGCGCTGACCCACCCGGTGGTCCTGCTCACCGGTCTGGTGGTG
CTCGCCCTGGCCATCGCCGGCAAGTTCGCCGGCGCGTTCGCCGGCGCGCGGCTGAGCGGG
TTGAACAAGTGGGAAGGGCTGGCGCTCGGCGCCGGGCTGAACGCGCGGGGAGTCATCCAG
GTCGTGGTGGCCATGGTCGGCCTGCGGCTGGGTATCCTCAGCGTGGAGGTCTACACGATC
ATCATCCTCGTCGCGATCGTCACTTCCCTGATGGCGTCGCCGATCCTGCGGTTCGCGATG
TCCAGAGTGGAGCAGACCGCCGAAGAACAGGTTCGCGAGAACGAACACCGGGCGTGGAAC
ACGCACCCGGCGGCGAACCCGCAGGAGCAAAGTCTCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006153 Cation/H+ exchanger (Domain) PF00999 Na_H_Exchanger
SignalP	No significant hit
TMHMM	Transmembrane 13

Balhi_00260 Balhi_00280