Balhi_00280 : CDS information

Location

Organism	Amycolatopsis balhimycina
Strain	DSM 5908
Entry name	Balhimycin
Contig	Y16952
Start / Stop / Direction	56,501 / 57,913 / + [in whole cluster] 56,501 / 57,913 / + [in contig]
Location	56501..57913 [in whole cluster] 56501..57913 [in contig]
Type	CDS
Length	1,413 bp (470 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase
Product (GenBank)	putative NDP-hexose 2,3-dehydratase
Gene
Gene (GenBank)	dvaA
EC number
Keyword	dehydrovancosamine
Note
Note (GenBank)
Reference	ACC Q939X7 comment Amycolatopsis balhimycina_dvaA Putative NDP-hexose 2,3-dehydratase このORFを同定した論文は不明。機能についての実験報告もなし。
Related Reference	ACC O52793 NITE Chlor_00240 PmId [11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000) [23473392] Structure of EvaA: a paradigm for sugar 2,3-dehydratases. (Biochemistry. , 2013) comment BLAST id90% Amycolatopsis orientalis (Nocardia orientalis) PCZA361.3 ORF23(EvaA)に相当するentry. --- [PMID: 11035791](2000) dTDP-4-keto-6-deoxy-D-glucoseからdTDP-L-epivancosamineを生合成する5つの酵素を、E.coliで発現・精製し、特徴づけしている。 ORF23はdTDP-6-deoxy-4-keto-D-glucoseを基質として、2,6-dideoxy-3,4-diketo-dTDP sugarの分解化合物をもたらした。ORF23とORF25の組み合わせは、C-3アミノ基がequatorialなdTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseをもたらした。すべての結果から提唱されている経路は次の通り。 dTDP-4-keto-6-deoxy-D-glucose ↓ ORF23(EvaA): C-2 deoxygenation ↓ ORF25(EvaB): C-3 amination dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-ulose ↓ ORF14(EvaC): C-3 methylation dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-ulose ↓ ORF26(EvaD): C-5 epimerization ↓ ORF24(EvaE): C-4 ketoreduction dTDP-L-epivancosamine --- [PMID: 23473392](2013) 本文未確認。 EvaAの構造解析。 EvaAは2量体で、2つのdTDP-sugar binding pockets A and Bが各subunitに存在する。site-directed mutagenesis実験と活性分析に基づき、pocket Aがactive siteとして機能し、pocket Bは遺伝子複製現象の残遺物であると思われる。

Sequence

>putative dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [putative NDP-hexose 2,3-dehydratase]
MLPDLVPPVVVRPRDGRDHADRIALSAATTDGVHMRTEDVRAWIAERREANDFHVERVPF
RDLDQWSFEEVTGNLVHHSGRFFTIEGLHVIEHDGPNGDGPYREWQQPVIKQPEVGILGI
LGKEFGGVLHFLMQAKMEPGNPNLVQLSPTVQATRSNYTKAHGGTNVKLIEYFAPPDPEH
VIVDVLQAEQGSWFFRKSNRNMIVETVDDVPLWDDFCWLTLGQIAELMHEDETINMNARS
VLSCLPYHDAAPGARFSDVQLLSWFTNERSRHDVRARRIPLADVCGWKQGDEAIEHEDGR
YFRVLAVAVRGSNRERISWTQPLLESVDLGVVAFLVREIGGVPHVLVHARADGGFLDTVE
LAPTVQCTPQNYAHLPAENRPPFLDVVLNAPESRIRYEAIHSEEGGRFLNVRARYLAIEA
DDTVEPPPGYTWVTPAQLTALTRHGHYVNVEARTLLACLNAATAQPRGGA

>putative dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [putative NDP-hexose 2,3-dehydratase]
ATGCTGCCTGACCTCGTTCCCCCGGTCGTGGTGCGCCCCCGCGACGGCCGCGACCACGCG
GACCGCATCGCGTTGTCGGCGGCGACCACCGACGGGGTGCACATGCGGACCGAGGACGTC
CGCGCCTGGATCGCCGAACGCCGTGAGGCCAACGACTTCCACGTCGAACGCGTCCCGTTC
CGGGACCTCGACCAGTGGTCGTTCGAGGAGGTGACCGGCAACCTCGTGCACCACAGCGGA
CGGTTCTTCACCATCGAGGGCCTGCACGTGATCGAGCACGACGGCCCGAACGGCGACGGC
CCCTACCGCGAGTGGCAGCAACCGGTCATCAAGCAGCCCGAAGTCGGCATCCTCGGCATC
CTGGGCAAGGAGTTCGGCGGCGTCCTGCACTTCCTGATGCAGGCCAAGATGGAGCCGGGG
AACCCCAATCTGGTGCAGCTCTCGCCGACCGTGCAGGCCACCCGCAGCAACTACACCAAG
GCGCACGGCGGCACGAACGTCAAGCTGATCGAGTACTTCGCCCCGCCCGACCCCGAGCAC
GTCATCGTCGACGTTCTCCAGGCCGAGCAAGGCTCGTGGTTCTTCCGCAAGTCCAATCGC
AACATGATCGTCGAGACCGTCGACGACGTGCCGCTGTGGGACGACTTCTGCTGGCTCACC
CTCGGCCAGATCGCGGAGCTGATGCACGAGGACGAGACGATCAACATGAACGCCAGGAGC
GTGTTGTCGTGCCTGCCTTACCACGACGCGGCTCCCGGCGCGCGGTTCTCCGACGTCCAG
CTCCTGTCGTGGTTCACGAACGAGCGTTCGCGGCACGACGTGCGTGCCCGCCGCATCCCG
CTCGCGGACGTGTGCGGCTGGAAGCAGGGCGACGAAGCGATCGAGCACGAGGACGGCCGT
TATTTCCGGGTCCTCGCGGTCGCCGTGCGGGGGAGCAACCGCGAGCGGATCAGCTGGACC
CAGCCGCTGCTCGAATCCGTCGACCTGGGTGTCGTCGCGTTCCTCGTGCGCGAGATCGGC
GGTGTGCCCCACGTTCTGGTGCACGCCCGCGCCGACGGTGGTTTCCTGGACACGGTCGAG
CTGGCACCGACCGTCCAGTGCACTCCCCAAAACTACGCGCACCTGCCCGCGGAGAACCGC
CCGCCCTTCCTCGACGTCGTCCTCAACGCTCCGGAGTCGCGCATTCGTTACGAGGCAATA
CATTCCGAAGAGGGCGGGCGCTTCCTCAACGTCCGGGCGCGCTACCTCGCGATCGAAGCG
GACGACACGGTCGAGCCCCCTCCCGGCTACACCTGGGTCACGCCGGCCCAGCTCACCGCG
CTCACCCGGCACGGGCACTACGTCAACGTCGAGGCCCGCACGCTGCTCGCCTGCCTCAAC
GCCGCGACGGCCCAGCCTCGAGGCGGTGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Balhi_00270 Balhi_00290