Balhi_00310 : CDS information

Location

Organism	Amycolatopsis balhimycina
Strain	DSM 5908
Entry name	Balhimycin
Contig	Y16952
Start / Stop / Direction	59,344 / 59,961 / + [in whole cluster] 59,344 / 59,961 / + [in contig]
Location	59344..59961 [in whole cluster] 59344..59961 [in contig]
Type	CDS
Length	618 bp (205 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-3-amino-4-keto-2,3,6-trideoxy-3-C-methyl-D-glucose 5-epimerase
Product (GenBank)	putative 3,5 epimerase
Gene
Gene (GenBank)	dvaD
EC number	5.1.3.-
Keyword	dehydrovancosamine
Note
Note (GenBank)
Reference	ACC Q939X4 comment Amycolatopsis balhimycina_dvaD Putative 3,5 epimerase このORFを同定した論文は不明。機能についての実験報告もなし。
Related Reference	ACC O52806 NITE Chlor_00270 PmId [11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000) [12077451] Purification, crystallization and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis, the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathway. (Acta Crystallogr D Biol Crystallogr. , 2002) [15159413] The position of a key tyrosine in dTDP-4-Keto-6-deoxy-D-glucose-5-epimerase (EvaD) alters the substrate profile for this RmlC-like enzyme. (J Biol Chem. , 2004) comment BLAST id78% Amycolatopsis orientalis (Nocardia orientalis) PCZA361.16 ORF26(EvaD)に相当するentry. --- [PMID: 11035791](2000) dTDP-4-keto-6-deoxy-D-glucoseからdTDP-L-epivancosamineを生合成する5つの酵素を、E.coliで発現・精製し、特徴づけしている。 ORF26でdTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-uloseを反応させると、分子量は変わらないがchromatographyだと明らかに異なる化合物をもたらしたことから、ORF26はC-5 epimeraseとして機能すると予測された。C-5での重水素の取り込みで、これを確認している。すべての結果から提唱されている経路は次の通り。 dTDP-4-keto-6-deoxy-D-glucose ↓ ORF23(EvaA): C-2 deoxygenation ↓ ORF25(EvaB): C-3 amination dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-ulose ↓ ORF14(EvaC): C-3 methylation dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-ulose ↓ ORF26(EvaD): C-5 epimerization ↓ ORF24(EvaE): C-4 ketoreduction dTDP-L-epivancosamine --- [PMID: 12077451] (2002) dTDP-3-amino-4-keto-2,3,6-trideoxy-3-C-methyl-glucose-5-epimerase (EvaD)の結晶構造解析。 --- [PMID: 15159413](2004) 結晶構造解析のグループの続報。EvaDの構造解析と生化学的特徴づけ。 EvaDはRmlCクラスの酵素なのにdouble epimerization活性がとても低いのは、触媒残基Tyrの位置に関係することをmutant作成などで確認している。

Sequence

>putative dTDP-3-amino-4-keto-2,3,6-trideoxy-3-C-methyl-D-glucose 5-epimerase [putative 3,5 epimerase]
MQARKLAVDGAIEFTPRVFPDDRGLFVSPFQEEAFAEARGGPLFRVAQTNHSMSKRGVVR
GIHYTMTPPGTAKYVYCARGKALDIVVDIRVGSPTFGRWDAVLLDQRDHRAMYFPVGVGH
AFVALEDDTAMWYLLSTAYVARNELALSVLDPALGLPIDADVDPILSERDQVAVTLAEAG
RQGLLPDYATCLELDRQLSEVSLSA

>putative dTDP-3-amino-4-keto-2,3,6-trideoxy-3-C-methyl-D-glucose 5-epimerase [putative 3,5 epimerase]
GTGCAAGCACGCAAACTCGCCGTCGACGGCGCGATCGAGTTCACCCCCCGGGTCTTCCCC
GACGACCGGGGCCTGTTCGTCTCGCCGTTCCAGGAAGAGGCCTTCGCCGAGGCCCGCGGC
GGCCCGCTGTTCCGGGTGGCGCAGACGAACCACAGCATGTCCAAGCGCGGCGTGGTGCGT
GGCATCCACTACACGATGACGCCACCGGGCACGGCCAAGTACGTCTACTGTGCCCGCGGC
AAGGCGTTGGACATCGTGGTCGACATCCGGGTCGGCTCGCCGACGTTCGGCCGGTGGGAC
GCGGTCCTGCTGGACCAGCGGGACCACCGGGCGATGTACTTCCCGGTGGGGGTCGGCCAC
GCGTTCGTGGCCCTCGAGGACGACACCGCCATGTGGTACCTGCTCTCCACGGCCTACGTG
GCGCGGAACGAGCTCGCCCTCTCGGTCCTGGATCCCGCGCTGGGCCTGCCCATCGACGCC
GACGTCGACCCGATCCTGTCCGAACGGGACCAGGTGGCCGTCACGCTCGCCGAGGCGGGA
CGGCAGGGGTTGCTGCCGGACTACGCCACCTGCCTGGAGCTCGACCGGCAGCTGTCCGAA
GTCTCCCTTTCCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family) PD001462 dTDP_sugar_isom PF00908 dTDP_sugar_isom IPR011051 RmlC-like cupin domain (Domain) SSF51182 RmlC_like_cupin IPR014710 RmlC-like jelly roll fold (Domain) G3DSA:2.60.120.10 RmlC-like_jellyroll
SignalP	No significant hit
TMHMM	No significant hit

Balhi_00300 Balhi_00320