Balhi_00300 : CDS information

Location

Organism	Amycolatopsis balhimycina
Strain	DSM 5908
Entry name	Balhimycin
Contig	Y16952
Start / Stop / Direction	58,211 / 59,320 / + [in whole cluster] 58,211 / 59,320 / + [in contig]
Location	58211..59320 [in whole cluster] 58211..59320 [in contig]
Type	CDS
Length	1,110 bp (369 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-hexose C-3 aminotransferase
Product (GenBank)	putative C-3 amino transferase
Gene
Gene (GenBank)	dvaB
EC number	2.6.1.-
Keyword	dehydrovancosamine
Note
Note (GenBank)
Reference	ACC Q939X5 comment Amycolatopsis balhimycina_dvaB Putative C-3 amino transferase このORFを同定した論文は不明。機能についての実験報告もなし。
Related Reference	ACC O52807 NITE Chlor_00260 PmId [11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000) comment BLAST id92% Amycolatopsis orientalis (Nocardia orientalis) PCZA361.5 ORF25(EvaB)に相当するentry. dTDP-4-keto-6-deoxy-D-glucoseからdTDP-L-epivancosamineを生合成する5つの酵素を、E.coliで発現・精製し、特徴づけしている。 ORF23はdTDP-6-deoxy-4-keto-D-glucoseを基質として、2,6-dideoxy-3,4-diketo-dTDP sugarの分解化合物をもたらした。ORF23とORF25の組み合わせは、C-3アミノ基がequatorialなdTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseをもたらした。 ORF25の補酵素としてPLPを使用。amino donorとして6つのアミノ酸を調査し、L-Gluが好まれた補助基質であると同定した。すべての結果から提唱されている経路は次の通り。 dTDP-4-keto-6-deoxy-D-glucose ↓ ORF23(EvaA): C-2 deoxygenation ↓ ORF25(EvaB): C-3 amination dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-ulose ↓ ORF14(EvaC): C-3 methylation dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-ulose ↓ ORF26(EvaD): C-5 epimerization ↓ ORF24(EvaE): C-4 ketoreduction dTDP-L-epivancosamine

Sequence

>putative dTDP-hexose C-3 aminotransferase [putative C-3 amino transferase]
MTTRVWDYQAEYRNERLDLLDAVETVFDSGQLVLGASVRGFEAEFAAYHGVGHCVGLDNG
TNAIKLGLQALGVGPGDEVITVSNTAAPTVVAIDGTGATPVFVDVREDDFLMDTGQVAAA
ITERTKCLLPVHLYGQCVDMAPLKDLAAKHGLSILEDCAQAHGARQNGTVAGSTGDAAAF
SFYPTKVLGAYGDGGATITSDESVDRRLRRLRYYGMDKQYYTLETPAHNSRLDEVQAEIL
RRKLKRLDTYVAARQAIAQRYVDGLGDTELKLPRTVPGNEHVYYVYVVRHPRRDDIIERL
KAYDIHLNISYPWPVHTMTGFAHLGYATGAFPVTEKLAGEIFSLPMYPALSADLQDKVIH
AVREVVSTL

>putative dTDP-hexose C-3 aminotransferase [putative C-3 amino transferase]
ATGACCACGCGTGTATGGGACTACCAGGCCGAATACCGGAACGAGCGGCTCGACCTGCTG
GACGCGGTCGAGACGGTCTTCGACTCGGGGCAGCTCGTGCTCGGGGCGAGCGTGCGTGGC
TTCGAGGCGGAATTCGCCGCGTACCACGGGGTCGGGCACTGCGTTGGCCTCGACAACGGG
ACGAACGCGATCAAGCTCGGCCTGCAGGCGCTGGGTGTCGGGCCGGGCGACGAGGTGATC
ACGGTGTCCAACACCGCCGCCCCGACCGTGGTCGCCATCGACGGCACCGGCGCCACGCCG
GTCTTCGTCGACGTCCGCGAGGACGACTTCCTGATGGACACCGGCCAGGTCGCGGCCGCG
ATCACCGAGCGCACCAAGTGCCTGCTGCCCGTGCACCTATACGGACAGTGCGTGGACATG
GCTCCGCTGAAGGACCTCGCCGCGAAGCATGGACTGTCCATTTTGGAGGACTGTGCCCAG
GCGCACGGGGCCCGGCAGAACGGAACGGTCGCGGGCTCGACCGGTGACGCGGCCGCGTTC
TCCTTCTACCCGACCAAGGTGCTCGGGGCGTACGGCGACGGCGGCGCGACCATCACCTCC
GACGAATCCGTGGACCGGCGGCTGCGGCGGCTGCGCTACTACGGCATGGACAAGCAGTAC
TACACGCTGGAAACGCCGGCCCACAACAGCCGGCTGGACGAGGTCCAGGCCGAGATCCTG
CGGCGCAAGCTCAAGCGGCTCGACACCTACGTCGCCGCCCGCCAGGCCATCGCCCAGCGC
TACGTCGACGGACTGGGCGACACGGAGCTGAAGCTGCCGCGGACCGTCCCCGGCAACGAG
CACGTGTACTACGTGTACGTCGTGCGCCACCCGCGACGTGACGACATCATCGAGCGCCTC
AAGGCGTACGACATCCACTTGAACATCAGCTATCCGTGGCCGGTGCACACCATGACCGGT
TTCGCCCACCTCGGCTACGCGACCGGCGCGTTCCCGGTCACCGAAAAACTGGCCGGCGAG
ATCTTCTCGCTGCCGATGTACCCCGCGCTTTCCGCCGACCTGCAGGACAAGGTCATCCAT
GCGGTGCGCGAGGTGGTGTCCACCCTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family) PIRSF000390 PLP_StrS PF01041 DegT_DnrJ_EryC1 IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain) G3DSA:3.40.640.10 PyrdxlP-dep_Trfase_major_sub1 IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain) G3DSA:3.90.1150.10 PyrdxlP-dep_Trfase_major_sub2 IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain) SSF53383 PyrdxlP-dep_Trfase_major
SignalP	No significant hit
TMHMM	No significant hit

Balhi_00290 Balhi_00310