Balhi_00350 Balhi_00350   Balhi_00370 Balhi_00370

Balhi_00360 : CDS information

close this sectionLocation

Organism
StrainDSM 5908
Entry nameBalhimycin
Contig
Start / Stop / Direction64,162 / 65,259 / + [in whole cluster]
64,162 / 65,259 / + [in contig]
Location64162..65259 [in whole cluster]
64162..65259 [in contig]
TypeCDS
Length1,098 bp (365 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative phospho-2-dehydro-3-deoxyheptonate aldolase
putative 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
putative DAHP synthase
Product (GenBank)putative alodlase
Genedahp
Gene (GenBank)ald
EC number2.5.1.54
Keyword
  • Bht
  • Hpg
  • Dpg
Note
Note (GenBank)
Reference
ACC
PmId
[17693715] The border sequence of the balhimycin biosynthesis gene cluster from Amycolatopsis balhimycina contains bbr, encoding a StrR-like pathway-specific regulator. (J Mol Microbiol Biotechnol. , 2007)
[20570618] Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster. (Metab Eng. , 2010)
comment
[PMID: 17693715](2007)
vancomycinにとても似たグリコペプチド系抗生物質balhimycinの生合成gene clusterの追加報告。
新たにVanS, VanR, VanY, Bbr, Pdh, Tba, Dahpを同定している。

Dahp(365aa) : DAHP synthase

配列解析から名付けている。
このORFがbalhimycin生合成gene clusterの右側の境界であるとされている。
dahpの上流にBbrは結合しなかった。

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[PMID: 20570618](2010)
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (dahp) と prephenate dehydrogenase (pdh)についての特徴づけ。

balhimycinのaglycone骨格となるアミノ酸(Bht, Hpg, Dpg)は、直接的または間接的にshikimate経路に由来する。shikimate経路の律速酵素Dahp synthaseをコードするdahpの追加コピーを発現した株は、グリコペプチド生産性が3倍に改善された。逆にdahpのdeletion mutant株はbalhimycin産生で有意な減少をもたらした。
Related Reference
ACC
Q6TZ04
PmId
comment
BLAST id60%
Amycolatopsis methanolica_aroF
Phospho-2-dehydro-3-deoxyheptonate aldolase (EC:2.5.1.54)

DAHP-synthaseを2つ(aroG, aroF)もっている。それぞれを発現させ、活性をみたり、何により阻害されるかを確認している。
aroG(DS1:plant-type DAHP synthase[Q6TZ03])
aroF(DS2:E. coli-type DAHP synthase[Q6TZ04])
ACC
P0AB91
PmId
[1682314] ( , )
[8778789] ( , )
[12126632] ( , )
comment
BLAST id53%
Escherichia coli (strain K12)_aroG
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (EC:2.5.1.54)

close this sectionSequence

selected fasta
>putative phospho-2-dehydro-3-deoxyheptonate aldolase [putative alodlase]
MAAMTHTVATTDLDNQRIERIVPLVTPALLHHELPLSATAAETVRKGRESVVRVLDGTDD
RLLVITGPCSIHDPAAALDYAGHLAAIAGEVAGDLLVVMRVYFEKPRTIGGWKGLINDPH
LDGTGDVNHGLRTARHLLLELAERGLPAACEWLDTTIPAYFADTVSWGAIGARTVESQNH
RMLASGLSMPVGFKNRRDGDITVAIDAIRAAAVRHVVPGVDPGGLPAILHTAGNPDCHVV
LRGGDGAPNHDSASVHKTLTALEAAGLPGRVVIDASHDNSGKDHHRQPLVAAEIAGQVEN
GRNGIVGVMLESNLRAGRQDLQPGRPPAYGQSITDACIDVPTTRTVLHGLAAAAAARRKL
GKQAS
selected fasta
>putative phospho-2-dehydro-3-deoxyheptonate aldolase [putative alodlase]
GTGGCCGCGATGACCCACACCGTCGCCACGACCGACCTCGACAACCAGCGCATCGAGCGG
ATCGTCCCCCTGGTCACCCCCGCCCTGCTGCATCACGAACTGCCGCTCAGCGCCACCGCG
GCCGAGACGGTGCGAAAGGGCCGCGAGAGCGTCGTCCGCGTCCTCGACGGCACGGACGAC
CGGCTGCTCGTGATCACCGGGCCGTGCTCCATCCACGACCCCGCCGCGGCGCTCGACTAC
GCCGGCCACCTCGCCGCCATCGCCGGCGAGGTCGCCGGCGACCTGCTCGTCGTCATGCGC
GTGTACTTCGAGAAACCCCGGACGATCGGCGGCTGGAAGGGGCTCATCAACGACCCCCAC
CTCGACGGCACCGGCGACGTCAACCACGGGCTGCGCACGGCCCGGCACCTCCTGCTGGAG
CTCGCCGAACGCGGCCTGCCCGCCGCGTGCGAATGGCTGGACACCACCATTCCCGCGTAC
TTCGCGGACACGGTCTCGTGGGGCGCCATCGGCGCCCGCACCGTGGAAAGCCAGAACCAC
CGCATGCTCGCCAGCGGCCTGTCCATGCCCGTCGGCTTCAAGAACCGCCGCGACGGCGAC
ATCACCGTCGCCATCGACGCGATCCGGGCCGCCGCGGTCCGCCACGTGGTCCCCGGCGTC
GACCCCGGCGGGTTGCCCGCCATCCTGCACACGGCGGGCAACCCGGACTGCCACGTCGTC
CTGCGCGGTGGTGACGGCGCGCCCAACCACGACTCCGCGTCCGTCCACAAGACACTGACC
GCGCTGGAGGCCGCGGGCCTGCCCGGCCGGGTGGTGATCGACGCCAGCCACGACAACAGC
GGCAAGGACCACCACCGCCAGCCCCTCGTCGCGGCCGAGATCGCGGGCCAGGTCGAGAAC
GGGCGGAACGGCATCGTCGGGGTGATGCTCGAGTCCAACCTCCGCGCCGGCCGCCAGGAC
CTCCAGCCGGGCCGTCCGCCGGCATACGGCCAGTCCATCACCGACGCCTGCATCGACGTC
CCCACCACCCGGACGGTCCTCCACGGCCTCGCCGCGGCGGCCGCGGCCCGGCGAAAGCTC
GGCAAGCAAGCAAGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006218 DAHP synthetase I/KDSA (Domain)
[53-346] 1.29999999999998e-79 PF00793
PF00793   DAHP_synth_1
IPR006219 DHAP synthase, class 1 (Family)
[1-362] PIRSF001361
PIRSF001361   DAHP_synthase
[14-358] TIGR00034
TIGR00034   AroFGH
IPR013785 Aldolase-type TIM barrel (Domain)
[17-361] 4.89999999999985e-129 G3DSA:3.20.20.70
G3DSA:3.20.20.70   Aldolase_TIM
SignalP No significant hit
TMHMM No significant hit
Balhi_00350 Balhi_00350   Balhi_00370 Balhi_00370
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