A4092_00130 : CDS information

Location

Organism	Nonomuraea sp.
Strain	ATCC 39727
Entry name	A40926
Contig	AJ561198
Start / Stop / Direction	15,271 / 15,861 / + [in whole cluster] 15,271 / 15,861 / + [in contig]
Location	15271..15861 [in whole cluster] 15271..15861 [in contig]
Type	CDS
Length	591 bp (196 aa)

Annotation

Category	4.4 resistance
Product	D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine dipeptidase DD-carboxypeptidase/DD-dipeptidase
Product (GenBank)	putative VanY-type carboxypeptidase
Gene	dbv7 vanYn
Gene (GenBank)	dbv7
EC number	3.4.13.22 3.4.16.4
Keyword
Note
Note (GenBank)
Reference	ACC Q7WZ84 PmId [12837387] The gene cluster for the biosynthesis of the glycopeptide antibiotic A40926 by nonomuraea species. (Chem Biol. , 2003) [17873036] Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926. (J Bacteriol. , 2007) [20308385] Novel mechanism of glycopeptide resistance in the A40926 producer Nonomuraea sp. ATCC 39727. (Antimicrob Agents Chemother. , 2010) [22788848] Characterization of VanYn, a novel D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance in Nonomuraea sp. ATCC 39727. (FEBS J. , 2012) [23497129] Streptomyces spp. as efficient expression system for a D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance. (BMC Biotechnol. , 2013) [24957828] Relationship between glycopeptide production and resistance in the actinomycete Nonomuraea sp. ATCC 39727. (Antimicrob Agents Chemother. , 2014) comment [PMID: 12837387](2003) Nonomuraea sp. ATCC 39727由来A40926生合成gene clusterの同定論文。 A40926は半合成グリコペプチドdalbavancin (BI397 or MDL 62,397)の前駆体。 dbv ORF7: carboxypeptidase このORFに関しては配列解析のみ。 --- [PMID: 17873036](2007) RT-PCRとその産物のシークエンスで、dbv5-dbv7が同時転写されることを確認。リン酸による遺伝子発現への影響がRT-PCR と real-time RT-PCRで調査されている。dbv6は影響を受けない。 --- [PMID: 20308385](2010) A40926を産生するNonomuraea sp. ATCC 39727におけるグリコペプチド耐性の調査。 Nonomuraea sp. ATCC 39727に存在するpeptidoglycan前駆体は、pentapeptideの末端D-Ala残基が除去されたtetrapeptideが優勢であり、これはdbv gene clusterにあるvanY(ORF7)のD,D-carboxypeptidase活性に基づくとされる。 vanY-null mutantはグリコペプチド耐性レベルが下がる。 Streptomyces venezuelaeにおけるvanYの異種性発現は、グリコペプチド耐性レベルを高める。 --- [PMID: 22788848](2012) E. coliで発現して得たVanY(n)を用いて活性確認。D,D-carboxypeptidase活性と D,D-dipeptidase活性を示すが、D-Ala-D-Lacで終結したペプチドでの D,D-carboxyesterase能力を欠く。VanY(n)の活性は非競合様式でβ-ラクタムにより抑制される。 --- 同じグループでさらに続報あり。 [PMID: 23497129](2013) [PMID: 24957828](2014)

Sequence

>D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine dipeptidase [putative VanY-type carboxypeptidase]
MRRSEGDDEPRTLPPRARDRVYTAVTRVLAVLLLPVAFVRQPGRARELACGWALRMRFPA
EDLTGLTDGARAAFTAARAEALWRHGQLVGLTSGYRDPRVQQRMFEEEVRRSGSVAAARM
FVAPPAESNHVKGMALDVRPHEGARWLEAHGARYDLYRIYDNEWWHFEHRPECGGTPPRR
LPHPGAAWASRNGGRV

>D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine dipeptidase [putative VanY-type carboxypeptidase]
ATGAGGAGAAGCGAGGGTGACGACGAACCACGCACTCTCCCGCCTCGGGCCCGGGACCGG
GTGTACACCGCGGTCACGCGGGTGCTCGCCGTGCTCCTGCTGCCCGTGGCGTTCGTCCGT
CAGCCCGGCCGCGCCCGCGAGCTGGCCTGCGGCTGGGCGTTGAGGATGCGATTCCCGGCA
GAGGACCTCACCGGGCTCACCGACGGCGCCAGGGCGGCGTTCACCGCGGCGCGGGCCGAG
GCGCTGTGGCGTCACGGCCAGCTCGTCGGTCTCACTTCCGGATACCGCGATCCCCGGGTC
CAGCAGCGGATGTTCGAGGAGGAGGTGCGCCGCTCAGGGTCCGTGGCCGCCGCACGGATG
TTCGTGGCGCCGCCGGCCGAGTCCAACCACGTCAAGGGCATGGCGCTGGACGTACGCCCG
CACGAGGGCGCGCGCTGGCTGGAGGCGCACGGCGCCCGCTACGACCTCTACCGCATCTAC
GACAACGAGTGGTGGCACTTCGAACACCGCCCGGAGTGCGGTGGCACGCCACCACGGCGG
CTACCCCACCCAGGCGCGGCCTGGGCGAGCCGGAACGGGGGCCGGGTCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003709 Peptidase M15B/M15C (Domain) PF02557 VanY IPR009045 Hedgehog signalling/DD-peptidase zinc-binding domain (Domain) SSF55166 Hedgehog_sig_N
SignalP	No significant hit
TMHMM	Transmembrane 1

A4092_00120 A4092_00140