Chlor_00150 : CDS information

Location

Organism	Amycolatopsis orientalis
Strain	A82846UV37B
Entry name	Chloroeremomycin
Contig	AJ223998
Start / Stop / Direction	40,739 / 41,965 / + [in whole cluster] 6,495 / 7,721 / + [in contig]
Location	40739..41965 [in whole cluster] 6495..7721 [in contig]
Type	CDS
Length	1,227 bp (408 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-3-amino-4-keto-2,3,6-trideoxy-D-glucose C-3 C-methyltransferase
Product (GenBank)	PCZA361.22
Gene	evaC ORF14
Gene (GenBank)
EC number	2.1.1.-
Keyword	L-4-epivancosamine
Note
Note (GenBank)	similar to hydroxylase
Reference	ACC P96561 PmId [9545426] Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic. (Chem Biol. , 1998) [11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000) comment [PMID: 9545426](1998) chloroeremomycin生合成 gene clusterの報告。 ORF14(408aa): Hydroxylase 配列解析のみ。過去にhydroxylaseとして同定されている（PMID: 9115410でhydroxylaseに相同なORFであると述べられている）。 --- [PMID: 11035791](2000) dTDP-4-keto-6-deoxy-D-glucoseからdTDP-L-epivancosamineを生合成する5つの酵素を、E.coliで発現・精製し、特徴づけしている。 ORF23はdTDP-6-deoxy-4-keto-D-glucoseを基質として、2,6-dideoxy-3,4-diketo-dTDP sugarの分解化合物をもたらした。ORF23とORF25の組み合わせは、C-3アミノ基がequatorialなdTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseをもたらした。さらにORF14を加えた組み合わせで補助基質としてSAMを使用すると、C-3メチル基がequatorialなdTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-uloseをもたらした。また、ORF14によるdTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseのC-methylationが、1.14ppmでのmethyl peakによって確認された。すべての結果から提唱されている経路は次の通り。 dTDP-4-keto-6-deoxy-D-glucose ↓ ORF23(EvaA): C-2 deoxygenation ↓ ORF25(EvaB): C-3 amination dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-ulose ↓ ORF14(EvaC): C-3 methylation dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-D-erythro-hexopyranos-4-ulose ↓ ORF26(EvaD): C-5 epimerization ↓ ORF24(EvaE): C-4 ketoreduction dTDP-L-epivancosamine

Sequence

>dTDP-3-amino-4-keto-2,3,6-trideoxy-D-glucose C-3 C-methyltransferase [PCZA361.22]
MSVTSQCRICDGTVKEFIDFGRQPLSDAFVVPGDEKGEFFFRLATGICDSCTMVQLMEEV
PRDLMFHDAYPYLSSGSAFMRTHFNELAKRLLTTELTGEDPFIVELGCNDGIMLKAMAEA
GVRQLGVEPSGSVADLAAAKGIRVRKDFFEEATAADIRETDGPADVIYAANTLCHIPYMD
SILKGVTTLLGPNGVFVFEDPYLGDIVERTSFDQIYDEHFFFFTARSVQEMAKRNGLELV
DVERIPVHGGEVRYTLALAGARQPTEAVAELLAWEAERKLAEYATLERFATNVKKIKDDL
IALLTKLRAEGKRVVGYGATAKSATVTNFCGITPDLVEFISDTTPAKQNRLSPGQHIPVR
EPKEFAADYPDYALLFAWNHADEIMNAEQAFRDAGGQWILYVPNVHVS

>dTDP-3-amino-4-keto-2,3,6-trideoxy-D-glucose C-3 C-methyltransferase [PCZA361.22]
ATGTCGGTCACGTCCCAGTGCCGTATCTGTGACGGCACTGTCAAAGAGTTCATCGATTTC
GGACGCCAGCCGCTCTCAGACGCGTTCGTCGTTCCCGGCGATGAGAAGGGTGAGTTCTTC
TTCCGGCTCGCCACCGGGATTTGCGATTCCTGCACGATGGTGCAGCTGATGGAAGAAGTC
CCGCGGGACCTGATGTTCCACGACGCGTATCCCTATCTGTCGTCCGGTTCGGCGTTCATG
CGCACGCATTTCAACGAGTTGGCCAAGCGCTTGCTGACCACGGAGCTGACCGGCGAGGAC
CCGTTCATCGTCGAGCTCGGCTGCAACGACGGCATCATGCTCAAGGCCATGGCCGAAGCG
GGTGTGCGCCAGCTCGGCGTCGAACCCTCCGGCAGCGTCGCGGATCTGGCCGCGGCCAAG
GGAATCCGCGTCCGCAAGGACTTCTTCGAAGAGGCGACGGCAGCCGACATCCGCGAGACC
GACGGCCCCGCCGACGTGATCTACGCGGCCAACACCCTGTGCCACATCCCTTACATGGAC
TCGATCCTGAAGGGCGTCACCACGCTGCTGGGCCCGAATGGCGTGTTCGTCTTCGAGGAC
CCGTACCTCGGTGACATCGTGGAGCGCACGTCGTTCGACCAGATCTACGACGAGCATTTC
TTCTTCTTCACCGCACGGTCGGTCCAGGAGATGGCCAAGCGCAACGGTCTTGAGCTCGTC
GACGTCGAGCGCATTCCGGTGCACGGCGGCGAGGTCCGCTACACCCTTGCCCTCGCTGGG
GCTCGTCAGCCGACCGAGGCCGTGGCGGAGCTTCTGGCATGGGAGGCCGAGCGCAAGCTG
GCGGAGTACGCCACGCTGGAACGCTTTGCCACCAACGTGAAGAAGATCAAGGACGACCTG
ATCGCGCTGCTGACCAAGCTGCGCGCCGAGGGCAAGCGCGTCGTCGGCTACGGCGCGACG
GCCAAGAGCGCCACCGTGACCAACTTCTGTGGCATCACCCCGGACCTGGTGGAGTTCATC
TCGGACACCACGCCGGCCAAGCAGAACAGGCTCAGCCCCGGCCAGCACATTCCGGTCCGC
GAGCCCAAGGAGTTCGCCGCCGACTACCCGGACTACGCCCTGCTGTTCGCGTGGAACCAC
GCCGACGAGATCATGAACGCGGAGCAGGCTTTCCGGGACGCCGGTGGGCAGTGGATCCTG
TACGTGCCGAACGTGCACGTGAGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013630 Methyltransferase putative zinc binding domain (Domain) PF08421 Methyltransf_13 IPR013691 C-methyltransferase (Domain) PF08484 Methyltransf_14
SignalP	No significant hit
TMHMM	No significant hit

Chlor_00140 Chlor_00160