Biological Resource Center, NITE (NBRC)

Microbial Genomic DNAs

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Genomic DNAs of the following microorganisms are available;
Fungi and Yeasts
NBRC No. Organism Strain Remarks
0622G Candida glabrata NBRC 0622   [NCBI]
1400G Candida tropicalis NBRC 1400   [NCBI]
1136G Saccharomyces cerevisiae NBRC 1136 = S288C [NCBI]
101557G Saccharomyces cerevisiae NBRC 101557 = Kyokai no.7 [DOGAN] *a
111645G Schizosaccharomyces pombe NBRC 111645 = L972 [NCBI]
The average length is over 10kbp.
31329G Trichoderma reesei NBRC 31329   [NCBI]
Bacteria NEW!!
NBRC No. Organism Strain Remarks
3283G Acetobacter pasteurianus NBRC 3283 = 46 [DOGAN][NCBI]
100883G Acidiphilium multivorum NBRC 100883 = AIU 301 [DOGAN]
102363G Actinoplanes missouriensis NBRC 102363T = 431 [NCBI]
The average length is over 10kbp.
15112G Amphibacillus xylanus NBRC 15112T = Ep01 [NCBI]
100420G Anaerolinea thermophila NBRC 100420T = UNI-1 [DOGAN]
105658G Armatimonas rosea NBRC 105658T = YO-36  
15305G Bacillus cereus NBRC 15305T   [NCBI]
13719G Bacillus subtilis subsp. subtilis NBRC 13719T    
100599G Brevibacillus brevis NBRC 100599 = 47 [DOGAN][NCBI]
104884G Burkholderia plantarii NBRC 104884T = AZ 8201 [NCBI]
100824G Caldanaerobacter subterraneus subsp. tengcongensis NBRC 100824T = MB4 [NCBI]
The average length is under 10kbp.
The minimum quantity is 500ng.
104410G Caldisericum exile NBRC 104410T = AZM16c01 [DOGAN]
13948G Clostridium acetobutylicum NBRC 13948T = 2291 [NCBI]
The average length is over 10kbp.
101661G Clostridium clariflavum NBRC 101661T   [NCBI]
12016G Clostridium kluyveri NBRC 12016T = K-1 [NCBI]
14293G Clostridium sporogenes NBRC 14293   The average length is over 10kbp.
103400G Clostridium thermocellum NBRC 103400T = VPI 7372 [NCBI]
100395G Corynebacterium efficiens NBRC 100395T = YS-314 [DOGAN] [NCBI]
12168G Corynebacterium glutamicum NBRC 12168T = 534 [NCBI]
107605G Cutibaterium acnes NBRC 107605T   [NCBI]
101012G Deferribacter desulfuricans NBRC 101012T = SSM1 [DOGAN][NCBI]
The average length is under 10kbp.
104933G Desulfovibrio magneticus NBRC 104933T = RS-1 [DOGAN][NCBI]
The average length is under 10kbp.
100478G Enterococcus casseliflavus NBRC 100478T = 20 [NCBI]
100678G NEW!! Enterococcus disper NBRC 100678T = E18-1
= Terumo1432
 
100479G Enterococcus durans NBRC 100479T   [NCBI]
100481G Enterococcus faecalis NBRC 100481T = Tissier  
100486G Enterococcus faecium NBRC 100486T = serotype 11 [NCBI]
100675G Enterococcus gallinarum NBRC 100675T = F87/276 [NCBI]
100491G NEW!! Enterococcus pseudoavium NBRC 100491T = 47-16 [NCBI]
100492G NEW!! Enterococcus raffinosus NBRC 100492T = CDC 1789-79 [NCBI]
107761G Escherichia albertii NBRC 107761T = R-14430 [NCBI]
12713G Escherichia coli NBRC 12713 = W3110 [NCBI]
105704G Escherichia hermannii NBRC 105704T   [NCBI]
The average length is over 10kbp.
102420G Escherichia vulneris NBRC 102420T   [NCBI]
The average length is over 10kbp.
100505G Gemmatimonas aurantiaca NBRC 100505T = T-27 [DOGAN] [NCBI]
13315G Geodermatophilus obscurus NBRC 13315T = G-20 [NCBI]
16485G Hydrogenobacter subterraneus NBRC 16485T = HGP1  
14216G Kitasatospora setae NBRC 14216T = KM-6054
= KCC A-0304
[DOGAN][NCBI]
14940G Klebsiella pneumoniae subsp. pneumoniae NBRC 14940T = KM 2924  
103217G Kocuria rhizophila NBRC 103217 = DC2201 [DOGAN][NCBI]
3288G Komagataeibacter medellinensis NBRC 3288T = 51 [NCBI]
Former Name: Gluconacetobacter xylinus
13120G Lactobacillus acetotolerans NBRC 13120 = RIB 9124
100934G Lactococcus garvieae NBRC 100934T = 0159 [NCBI]
100676G NEW!! Lactococcus lactis subsp. cremoris NBRC 100676T   [NCBI]
100931G NEW!! Lactococcus lactis subsp. hordniae NBRC 100931T = HC-1 [NCBI]
101784G Microlunatus phosphovorus NBRC 101784T = NM-1 [DOGAN][NCBI]
14854G Mycoplasma fermentans NBRC 14854T = PG18 [NCBI]
The minimum quantity is 500ng.
16S-23S ITS region was checked by PCR method.
14477G Mycoplasma orale NBRC 14477T = CH19299 The minimum quantity is 500ng.
16S-23S ITS region was checked by PCR method.
14401G Mycoplasma pneumoniae NBRC 14401T = FH [NCBI]
The minimum quantity is 500ng.
16S-23S ITS region was checked by PCR method.
The average length is over 10kbp.
14896G Mycoplasma pulmonis NBRC 14896T   The minimum quantity is 500ng.
16S-23S ITS region was checked by PCR method.
14298G Nitrosomonas europaea NBRC 14298 = NS-5-2 [NCBI]
The minimum quantity is 500ng.
14402G Nocardia brasiliensis NBRC 14402T = 337 [NCBI]
102666G Phycisphaera mikurensis NBRC 102666 = FYK2301M01 [NCBI]
106052G Pseudomonas aeruginosa NBRC 106052 = PAO1 [NCBI]
100887G Rhodococcus erythropolis NBRC 100887 = PR4 [DOGAN][NCBI]
108011G Rhodococcus opacus NBRC 108011 = B-4 [DOGAN] *a
100245G Rubrivivax gelatinosus NBRC 100245 = IL144 [NCBI]
100952G Rubrobacter xylanophilus NBRC 100952T = PRD-1 [NCBI]
The average length is over 10kbp.
13245G Salmonella enterica subsp. enterica NBRC 13245T = LT 2
= TM 2
[NCBI]
105725G Shimwellia blattae NBRC 105725T = CDC 9005-74 [NCBI]
101211G Sphingobium japonicum NBRC 101211 = UT26S [DOGAN][NCBI]
103272G Sphingobium sp. NBRC 103272 = SYK-6 [DOGAN][NCBI] *a
13955G Streptococcus mutans NBRC 13955T   [NCBI]
14893G Streptomyces avermitilis NBRC 14893T = MA-4680 [DOGAN][NCBI]
The average length is under 10kbp.
13350G Streptomyces griseus NBRC 13350   [NCBI]
3776G Streptosporangium roseum NBRC 3776   [NCBI]
12172G Tetragenococcus halophilus NBRC 12172 = d-6 [DOGAN][NCBI]
103674G Thermosulfidibacter takaii NBRC 103674T = ABI70S6 The average length is over 10kbp.
100826G Thermotoga maritima NBRC 100826T = MSB8 [NCBI]
The average length is over 10kbp.
101084G Thermus thermophilus NBRC 101084T = HB8 [NCBI]
103022G Vibrio vulnificus NBRC 103022 = P1104-1  
Archaea
NBRC No. Organism Strain Remarks
100138G Aeropyrum pernix NBRC 100138T = K1 [DOGAN] [NCBI]
100126G Archaeoglobus fulgidus NBRC 100126T = VC-16 [NCBI]
The average length is over 10kbp.
100127G Archaeoglobus profundus NBRC 100127T = AV18 [NCBI]
The average length is under 10kbp.
101032G Haloarcula japonica NBRC 101032T = TR-1 [NCBI]
100440G Methanocaldococcus jannaschii NBRC 100440T = JAL-1 [NCBI]
The average length is over 10kbp.
101707G Methanocella paludicola NBRC 101707T = SANAE [DOGAN][NCBI]
103675G Methanosaeta concilii NBRC 103675T = GP6 [NCBI]
101360G Methanosaeta thermophila NBRC 101360T = PT [NCBI]
100939G Methanosarcina acetivorans NBRC 100939T = C2A [NCBI]
The average length is over 10kbp.
100474G Methanosarcina barkeri NBRC 100474T = MS Reference: Genome sequence of M. barkeri Fusaro [NCBI]
100330G Methanothermobacter thermautotrophicus NBRC 100330T = ΔH [NCBI]
The average length is under 10kbp.
100828G Picrophilus torridus NBRC 100828T = KAW 2/3 [NCBI]
The average length is under 10kbp.
100827G Pyrobaculum aerophilum NBRC 100827T = IM2 [NCBI]
100139G Pyrococcus horikoshii NBRC 100139T = OT3 [DOGAN] [NCBI]
100439G Pyrodictium abyssi NBRC 100439T = AV 2 The minimum quantity is 500ng.
15157G Sulfolobus acidocaldarius NBRC 15157T   [NCBI]
15437G Sulfolobus shibatae NBRC 15437T = B 12  
100140G Sulfolobus tokodaii NBRC 100140T = 7 [DOGAN] [NCBI]
15161G Sulfurisphaera ohwakuensis NBRC 15161T = TA 1  
101555G Thermococcus celericrescens NBRC 101555T = TS2 The average length is under 10kbp.
15155G Thermoplasma acidophilum NBRC 15155T = 122-1B2 [NCBI]
The minimum quantity is 500ng.
The average length is over 10kbp.
15438G Thermoplasma volcanium NBRC 15438T = GSS 1 [NCBI]
100435G Thermoproteus tenax NBRC 100435T = Kra 1 [NCBI]
The minimum quantity is 500ng.
100879G Vulcanisaeta souniana NBRC 100879T = IC-059 The average length is under 10kbp.
*a:
The covenant is required. In response to your order, we will send you it by e-mail. Please fill out and send it back to us by postal mail, not e-mail or Fax. And a handling charge of 1,000 JPY is added for distribution of the samples.

Quality of genomic DNAs

The microbial genomic DNA supplied by NBRC is checked by the following quality verification procedure.

Quantity
The quantity (over 1µg) is checked by spectrophotometry.
Suitability for enzyme reaction
The suitability for enzyme reaction is checked by the PCR.
Sequencing of the 16S rDNA
The full-region of 16S rDNA is sequenced.
DNA Length
The average length (over 20kbp) is checked by pulse field gel electrophoresis (PFGE).

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Ordering Genomic DNAs

1. Distribution Policy

Distribution of the genomic DNA to domestic and foreign institutions will be subject to the regulations of Japanese Ministries. We shall ship our microbial genomic DNA to all countries except for those restricted by Japanese government.

2. Ordering Form

Ordering Form download [PDF:194KB]

After downloading the Ordering Form, please read the attached "Agreement of Biological Resource Transfer and Treatment". If you don't agree with any of the items, we will be unable to accept your order.
Please note both the name(s) of DNA resource(s) and its (their) NBRC number(s) + G in your order, and sign or place a seal in the signature column. (See also the example of description [PDF:37KB])

3. Inquiry to NBRC

Please complete the order form and send it to the following address by postal mail, facsimile or e-mail. Order by telephone is currently not accepted. If your government agency requires a document for importing our genomic DNA resource(s), please send us a necessary form or forms together with the Ordering form. In response to your order, we will send a proforma invoice by e-mail and start to process your order.

Biological Resource Center (NBRC),
National Institute of Technology and Evaluation
2-5-8 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818 JAPAN
FAX:
+81-438-52-2329
E-mail:
dna@nite.go.jp

4. Payment

For price list, please click here.
We accept payment by bank remittance or credit card (VISA or Master). We do not accept payment by cash or check.

5. Shipping

We can ship the DNA resource(s) after verifying your payment.
We dispatch the DNA resource(s) as a dried pellet in a plastic tube by Express Mail Service (EMS).

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Notice

Depending on the culture, preservation conditions, judicial regulations, etc., DNA resource(s) will be either removed from the distribution list or modified for their distribution conditions without prior notice.

As stated in the "Agreement of Biological Resource Transfer and Treatment" mentioned above, the DNA resource(s) you have received from us should not be transferred to a third party.

We do not disclose your personal information to any third parties. If you have any questions concerning our DNA resources and/or related matters, please send an e-mail to: dna@nite.go.jp. We cannot answer to your questions by telephone.

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Contact us

 Culture Collection Division,  Biological Resource Center,  National Institute of Technology and Evaluation
Phone number:+81-438-20-5763
Fax number:+81-438-52-2329
Address:2-5-8 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818 JAPAN MAP
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