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Medium Detail Information

Medium No.
1008
Medium
Pelobacter carbinolicus Medium
Composition
 KH2PO40.2g
 NH4Cl0.25g
 NaCl20g
 MgCl2·6H2O3g
 KCl0.5g
 CaCl2·2H2O0.15g
 Trace element solution SL-10*1ml
 Resazurin1mg
 NaHCO32.5g
 2,3-Butanediol0.9g
 Na2S·9H2O0.36g
 Distilled water1L
 pH to 7.2 
  Mix ingredients except for 2,3-butanediol and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20), seal with butyl rubber stoppers, and autoclave at 121°C for 20 min. Separately autoclave Na2S·9H2O under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add filter-sterile 2,3-butanediol and Na2S·9H2O solutions.
       
  *Trace element solution SL-10:
   HCl (25%; 7.7 M)10ml
   FeCl2·4H2O1.5g
   ZnCl270mg
   MnCl2·4H2O100mg
   H3BO36mg
   CoCl2·6H2O190mg
   CuCl2·2H2O2mg
   NiCl2·6H2O24mg
   Na2MoO4·2H2O36mg
   Distilled water990ml
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