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Medium Detail Information

Medium No.	1016
Medium	Pelobacter acetylenicus Medium
Composition
KH2PO4 0.2 g   NH4Cl 0.25 g   NaCl 1 g   MgCl2·6H2O 0.4 g   KCl 0.5 g   CaCl2·2H2O 0.15 g   Trace element solution SL-10* 1 ml   Resazurin 1 mg   NaHCO3 2.5 g   Acetoin 1 g   Na2S·9H2O 0.36 g   Distilled Water 1 L   pH to 7.2       Mix ingredient except for acetoin and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers. Separately autoclave Na2S·9H2O (3.6% solution) under a N2 atmosphere. Aseptically and anaerobically add Na2S·9H2O solution. Just prior to inoculation, add filter-sterile acetoin solution.                   *Trace element solution SL-10:       HCl (25%; 7.7 M) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 6 mg       CoCl2·6H2O 190 mg       CuCl2·2H2O 2 mg       NiCl2·6H2O 24 mg       Na2MoO4·2H2O 36 mg       Distilled water 990 ml
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